Difference between revisions of "Team:CCU Taiwan/Parts"
Allen850413 (Talk | contribs) |
|||
| (5 intermediate revisions by 2 users not shown) | |||
| Line 77: | Line 77: | ||
</head> | </head> | ||
| + | <body> | ||
<header> | <header> | ||
<nav> | <nav> | ||
| Line 91: | Line 92: | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a> | ||
| + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements"><li class="list" id="home5">Achievements</li></a> | ||
</ul> | </ul> | ||
</li> | </li> | ||
| Line 129: | Line 131: | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a> | ||
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/ | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Integrate"><li class="list" id="human_practice5">Integrated HP</li></a> |
</ul> | </ul> | ||
</li> | </li> | ||
| Line 167: | Line 169: | ||
<br> | <br> | ||
<p class="first" id="ca1">Background</p> | <p class="first" id="ca1">Background</p> | ||
| − | <p class="description">   | + | <p class="description">   “LIGGREEN” is composed of monolignols. We have produced three enzymes to catalyze the polymerization. Laccase 1 from Pinus taeda can catalyze the dehydrogenation of monolignols , which leads to free radical addition of dehydrogenated monolignols. However, after the monolignols become dimers or oligomers, it will be hard for them to bind onto the active site of laccase 1 and stop the dehydrogenation reaction of monolignols. |
| − | In this case, we choose the others enzymes | + | In this case, we choose the others enzymes-peroxidase 16 and peroxidase 18. Those are isoenzymes from Picea abies, have the ability to bind onto oligomers and directly catalyze polymerization.<br><br> |
  All the enzymes above can function in the same pH value and buffer, which is critical to our design for co-reaction in production line. |   All the enzymes above can function in the same pH value and buffer, which is critical to our design for co-reaction in production line. | ||
| Line 185: | Line 187: | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2809001">BBa_K2809001</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2809001">BBa_K2809001</a></td> | ||
<td>Ribosome binding site</td> | <td>Ribosome binding site</td> | ||
| − | <td>Kozak sequence (Pichia pastoris)</td> | + | <td>Kozak sequence (<I>Pichia pastoris</I>)</td> |
<td>7</td> | <td>7</td> | ||
</tr> | </tr> | ||
| Line 191: | Line 193: | ||
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2809002">BBa_K2809002</a></td> | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2809002">BBa_K2809002</a></td> | ||
<td>Tag</td> | <td>Tag</td> | ||
| − | <td>Prepro-alpha-factor (codon modified for Pichia pastoris)</td> | + | <td>Prepro-alpha-factor (codon modified for <I>Pichia pastoris</I>)</td> |
<td>267</td> | <td>267</td> | ||
</tr> | </tr> | ||
Latest revision as of 08:47, 1 December 2018
PARTS OVERVIEW
Background
“LIGGREEN” is composed of monolignols. We have produced three enzymes to catalyze the polymerization. Laccase 1 from Pinus taeda can catalyze the dehydrogenation of monolignols , which leads to free radical addition of dehydrogenated monolignols. However, after the monolignols become dimers or oligomers, it will be hard for them to bind onto the active site of laccase 1 and stop the dehydrogenation reaction of monolignols.
In this case, we choose the others enzymes-peroxidase 16 and peroxidase 18. Those are isoenzymes from Picea abies, have the ability to bind onto oligomers and directly catalyze polymerization.
All the enzymes above can function in the same pH value and buffer, which is critical to our design for co-reaction in production line.
Basic Parts
| Part Name | Part type | Description | Lenght |
|---|---|---|---|
| BBa_K2809001 | Ribosome binding site | Kozak sequence (Pichia pastoris) | 7 |
| BBa_K2809002 | Tag | Prepro-alpha-factor (codon modified for Pichia pastoris) | 267 |
| BBa_K2809010 | Protein_Domain | Mature Lac 1(laccase) | 1719 |
| BBa_K2809020 | Protein_Domain | Mature Px18(peroxidase) | 954 |
| BBa_K2809030 | Protein_Domain | Mature Px16(peroxidase) | 972 |
Composite Parts
| Part Name | Part type | Description | Lenght |
|---|---|---|---|
| BBa_K2809011 | Composite | Laccase 1 | 1993 |
| BBa_K2809021 | Peroxidase 18 | 1228 | |
| BBa_K2809031 | Peroxidase 16 | 1246 |