Difference between revisions of "Team:CCU Taiwan/Basic Part"

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{{CCU_Taiwan}}
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<html>
 
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<div class="column full_size judges-will-not-evaluate">
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<meta name="viewport" content="width=device-width, initial-scale=1">
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<h3>★  ALERT! </h3>
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<script>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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$(document).ready(function(){
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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</div>
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$("#Home").hover(function(e){
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$("#sub_home").stop(true,false,true).slideToggle(300);
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$("#Project").hover(function(){
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$("#sub_project").stop(true,false,true).slideToggle(300);
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});
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$("#Parts").hover(function(){
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$("#sub_parts").stop(true,false,true).slideToggle(300);
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$("#Modeling").hover(function(){
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$("#Drylab").hover(function(){
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<div class="column full_size">
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// Set the offset when entering page with hash present in the url
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<h1>Basic Parts</h1>
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window.setTimeout(offsetAnchor, 0);
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<p>
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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$(document).scroll(function () { // remove "$"
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    var y = $(this).scrollTop();       
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    if (y > 300) {
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        $('.indicator').fadeIn();
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    else {
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        $('.indicator').fadeOut();
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</script>
  
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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</head>
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</div>
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<body>
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  <header>
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        <nav>
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            <div class="container">
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                <ul class="front">
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                    <div id="home_button" style="cursor:pointer;"  onclick="location.href=
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'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div>
  
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<div class="column full_size">
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                    <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a>
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<div class="highlight decoration_background">
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                        <ul class="sub" id="sub_home" style="cursor:default;">
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<h3>Note</h3>
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a>
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a>
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</div>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a>
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</div>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements"><li class="list" id="home5">Achievements</li></a>
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                        </ul>
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                    </li>
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                    <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a>
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<ul class="sub" id="sub_project" style="cursor:default;">
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    <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Applied_Design"><li class="list" id="project2">Design</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab"><li class="list" id="project5">InterLab</li></a>
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</ul>
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</li>
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                    <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a>
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                        <ul class="sub" id="sub_parts" style="cursor:default;">
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Parts"><li class="list" id="parts1">Overview</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a>
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                        </ul>
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                    </li>
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                    <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a>
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                        <ul class="sub" id="sub_modeling" style="cursor:default;">
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a>
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding Model</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer Model</li></a>
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                        </ul>
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                    </li>
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                    <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a>
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                        <ul class="sub" id="sub_drylab" style="cursor:default;">
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering"><li class="list" id="drylab2">Production Line</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Safety"><li class="list" id="drylab3">Safety</li></a>
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                        </ul>
 +
                    </li>
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    <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a>
 +
                        <ul class="sub" id="sub_human_practice" style="cursor:default;">
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Human Practice</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Integrate"><li class="list" id="human_practice5">Integrated HP</li></a>
 +
                        </ul>
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                    </li>
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    <li class="title" style="cursor:pointer;" id="Notebook"><img class="img_title" src="https://static.igem.org/mediawiki/2018/c/c9/T--CCU_Taiwan--notebook.png"></img><a>Notebook</a>
 +
                        <ul class="sub" id="sub_notebook" style="cursor:default;">
 +
                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook"><li class="list" id="notebook1">Overview</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Collaborations"><li class="list" id="notebook2">Collaborations</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Protocols"><li class="list" id="notebook3">Protocols</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments"><li class="list" id="notebook4">Experiments</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Materials"><li class="list" id="notebook5">Materials</li></a>
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                        </ul>
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                    </li>
 +
                </ul>
 +
            </div>
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        </nav>
  
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    </header>
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<div class="indicator">
  
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<div class="column full_size">
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<div class="pointerProject" id="2"><a href="#ca1">Kozak sequence</a></div>
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<h3>Best Basic Part Special Prize</h3>
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<div class="pointerProject" id="3"><a href="#ca2">Prepro-alpha-factor</a></div>
 +
<div class="pointerProject" id="4"><a href="#ca3">Mature Lac1</a></div>
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<div class="pointerProject" id="3"><a href="#ca4">Mature Px18</a></div>
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<div class="pointerProject" id="4"><a href="#ca5">Mature Px16</a></div>
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</div>
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<div class="backgroundParts">
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<div class="photoBasicPart"><h1 class="bigtitle">BASIC PART<h1></div>
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<div class="content"><br><br>
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<p class="second" id="ca1"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2809001">BBa_K2809001</a> <br>
 +
Kozak sequence (<I>Pichia pastoris</I>)
 +
</p>
 +
<p class="description">&emsp;&emsp;The Kozak sequence initiates the translation in <I>Pichia pastoris</I>. This Sequence is copied directly from pCore1 in reference. The start codon is not included.</p>
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 +
<p class="second" id="ca2"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2809002">BBa_K2809002</a><br>
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Prepro-alpha-factor (codon modified for <I>P. pastoris</I>)
 +
</p>
 +
<p class="description">&emsp;&emsp;Prepro-alpha-factor is the secretion signal peptide from the yeast α-mating factor. This signal peptide directs expression of secretion of the recombinant protein, therefore, it allows the exportation of it. Once the recombinant protein is in the endoplasmic reticulum (ER) lumen, this secretion signal will be cleaved.</p><br><br>
 +
 +
<p class="second" id="ca3"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2809010">BBa_K2809010</a><br>
 +
Mature Lac1 (laccase)
 +
</p>
 +
<p class="description">&emsp;&emsp;This is the codon-optimized version of laccase derived from <I>Pinus taeda</I> (Loblolly pine). Laccase is involved in monolignol dehydrogenation, using oxygen to oxidize their metal centers to enable catalytical phenol oxidation. It is able to produce dimers and oligomers of coniferyl alcohol, which is involved in the early stage of lignification.</p>
  
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<p> To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
 
  
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<br><br>
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<div id="Basic1" class="polaroid">
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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                  <img src="https://static.igem.org/mediawiki/2018/a/af/T--CCU_Taiwan--CCUmodelbasicpsrt.png" style="width:100%">
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                  <div class="container">
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                    <p>Figure 1: Three resonance forms of coniferyl alcohol with radical.</p>
 +
                  </div>
 +
                </div>
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<div id="Basic2" class="polaroid" style="display:inline-block">
 +
                  <img src="https://static.igem.org/mediawiki/2018/9/9b/T--CCU_Taiwan--CCUmodelbasicpsrt2.png" width="100%">
 +
                  <div class="container">
 +
                    <p>Figure2: Target gene (laccase)<br>The signal peptide has been removed, so the sequence does NOT contain a start codon. Furthermore, it includes HA-tag for Western Blot protein detection and 6*His-tag for protein purification before the stop codon.</p>
 +
                  </div>
 
</div>
 
</div>
 +
<br><br>
 +
<p class="second"  id="ca4"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2809020">BBa_K2809020</a><br>
 +
Mature Px18 (peroxidase)
 +
</p>
 +
<p class="description">&emsp;&emsp;These are the codon-optimized versions of peroxidase derived from <I>Picea abies</I> (Norway spruce). Peroxidases (Px16 and Px18) are involved in monolignol dehydrogenation, using hydrogen peroxide as a substrate. They are able to form polymers from oligomers or dimers, which contribute to lignin polymerization.</p>
 +
<div id="Basic3" class="polaroid">
 +
                  <img src="https://static.igem.org/mediawiki/2018/2/28/T--CCU_Taiwan--CCUmodelbasicpsrt3.png" style="width:100%">
 +
 
 +
                  <div class="container">
 +
                    <p>Figure3: Three form of dimers.</p>
 +
                  </div>
 +
                </div>
  
  
 +
                <div id="Basic4" class="polaroid" style="display:inline-block">
 +
                  <img src="https://static.igem.org/mediawiki/2018/c/c0/T--CCU_Taiwan--CCUmodelbasicpsrt4.png" width="100%">
 +
                  <div class="container">
 +
                    <p>Figure4: Target gene (peroxidase 16)<br>The signal peptide has been removed, so the sequence does NOT contain a start codon. Moreover, it includes V5-tag for Western Blot protein detection and 6*His-tag for protein purification before the stop codon.</p>
 +
                  </div>
 +
                </div>
 +
<br><br>
 +
 +
<p class="second" id="ca5"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2809030">BBa_K2809030</a><br>
 +
Mature Px16 (peroxidase)</p>
 +
 +
<div class="row"> 
 +
<div id="halftext3"><p class="description">&emsp;&emsp;The function of peroxidase 16 is similar to peroxidase 18.</p></div>
 +
 +
                <div id="Basic5" class="polaroid" style="display:inline-block">
 +
                  <img src="https://static.igem.org/mediawiki/2018/8/8e/T--CCU_Taiwan--CCUmodelbasicpsrt5.png" width="100%">
 +
                  <div class="container">
 +
                    <p>Figure5: Target gene (peroxidase 18)<br>The signal peptide has been removed, so the sequence does NOT contain a start codon. Furthermore, it includes FLAG-tag for Western Blot protein detection and 6*His-tag for protein purification before the stop codon.</p>
 +
                  </div>
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                </div>
 +
</div>
 +
<br><br><br>
 +
</div>
 +
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<footer class="footer">
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Latest revision as of 08:47, 1 December 2018

BASIC PART



BBa_K2809001
Kozak sequence (Pichia pastoris)

  The Kozak sequence initiates the translation in Pichia pastoris. This Sequence is copied directly from pCore1 in reference. The start codon is not included.

BBa_K2809002
Prepro-alpha-factor (codon modified for P. pastoris)

  Prepro-alpha-factor is the secretion signal peptide from the yeast α-mating factor. This signal peptide directs expression of secretion of the recombinant protein, therefore, it allows the exportation of it. Once the recombinant protein is in the endoplasmic reticulum (ER) lumen, this secretion signal will be cleaved.



BBa_K2809010
Mature Lac1 (laccase)

  This is the codon-optimized version of laccase derived from Pinus taeda (Loblolly pine). Laccase is involved in monolignol dehydrogenation, using oxygen to oxidize their metal centers to enable catalytical phenol oxidation. It is able to produce dimers and oligomers of coniferyl alcohol, which is involved in the early stage of lignification.

Figure 1: Three resonance forms of coniferyl alcohol with radical.

Figure2: Target gene (laccase)
The signal peptide has been removed, so the sequence does NOT contain a start codon. Furthermore, it includes HA-tag for Western Blot protein detection and 6*His-tag for protein purification before the stop codon.



BBa_K2809020
Mature Px18 (peroxidase)

  These are the codon-optimized versions of peroxidase derived from Picea abies (Norway spruce). Peroxidases (Px16 and Px18) are involved in monolignol dehydrogenation, using hydrogen peroxide as a substrate. They are able to form polymers from oligomers or dimers, which contribute to lignin polymerization.

Figure3: Three form of dimers.

Figure4: Target gene (peroxidase 16)
The signal peptide has been removed, so the sequence does NOT contain a start codon. Moreover, it includes V5-tag for Western Blot protein detection and 6*His-tag for protein purification before the stop codon.



BBa_K2809030
Mature Px16 (peroxidase)

  The function of peroxidase 16 is similar to peroxidase 18.

Figure5: Target gene (peroxidase 18)
The signal peptide has been removed, so the sequence does NOT contain a start codon. Furthermore, it includes FLAG-tag for Western Blot protein detection and 6*His-tag for protein purification before the stop codon.