Difference between revisions of "Team:CCU Taiwan/Model"

 
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    <li class="title"><a href="https://2018.igem.org/Team:CCU_Taiwan/Description">Description</a></li>
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    <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a>
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<li class="title"><a href="https://2018.igem.org/Team:CCU_Taiwan/Design">Design</a></li>
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<li class="title"><a href="https://2018.igem.org/Team:CCU_Taiwan/Results">Results</a></li>
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    <li class="title"><a href="https://2018.igem.org/Team:CCU_Taiwan/Parts">Parts</a></li>
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                     <li class="title"><a href="#">Modeling</a>
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                            <li class="title"><a href="https://2018.igem.org/Team:CCU_Taiwan/Model">Model</a></li>
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                            <li class="title"><a href="https://2018.igem.org/Team:CCU_Taiwan/Enzyme">Enzyme Activity</a></li>
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<li class="title"><a href="https://2018.igem.org/Team:CCU_Taiwan/Monolignol">Monolignol Binding</a></li>
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<li class="title"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering">Engineering</a></li>
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<li class="title"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engagement">Engagement</a></li>
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<div class="pointerModeling" id="2"><a href="#ca2">Polymer model</a></div>
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<div class="photoModeling"><h1 class="bigtitle">MODELING<h1></div>
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      <div class="content">
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<br>
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<p class="description">&emsp;&emsp;In the future, there will be fewer and fewer petrochemical resources on the planet, but plastic waste are keeping accumulating. When things change, what should we do? To solve the problem, we use LIGGREEN. We hope to establish a new production line for laminating paper products using biological materials. First, we use natural enzymes to synthesize the material. Through the synthetic reaction of enzymes and monolignols, we ensure our LIGGREEN structure is similar to natural compounds. This allows us to create a biological laminate which will not require petrochemicals and high energy consumption. Our modeling is mainly divided into two parts to prove the feasibility of our project: binding model and polymer model.</p><br>
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<p class="first" id="ca1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Binding">Binding model</a></p>
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<div class="row"> 
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<div id="halftext3">
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<p class="description">&emsp;&emsp;In our experiment, coniferyl alcohol would form resonance structure after dehydrogenation, these resonance structures would form dimers (β-5, β-O-4, β-β). These reactions are catalytic by the enzymes and the addition of water.<br>
 +
<strong>Modeling:</strong> we decided to confirm the feasibility of the reaction through Gibbs free energy calculation. (Calculation method using Spartan 16)
 +
</p></div>
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                <div id="Model1" class="polaroid" style="display:inline-block">
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                  <img src="https://static.igem.org/mediawiki/2018/b/be/T--CCU_Taiwan--CCUmodel111.png" width="100%">
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                  <div class="container">
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                    <p>Figure1: Activation energy diagram when reaction is spontaneous.</p>
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                  </div>
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                </div>
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</div>
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<p class="first" id="ca2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer">Polymer model</a></p>
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<p class="description"> &emsp;&emsp;We produce three enzymes, Px16, Px18 and Lac1. LIGGREEN is produced by coniferyl alcohol and enzymes. The goal of Polymer model is to estimate the polymerization between coniferyl alcohol and enzymes.<br>
 +
<strong>Modeling:</strong> We use Flory-Stockmayer theory to estimate the polymerization. Through the theory, we can control some conditions to do the oligomerization and let LIGGREEN be more biodegradation and chain-like.
 +
</p>
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<br><br><br>
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             <a href="http://huitong.com.tw">
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            <p class="description">In order to realize the lignin-laminated paper cup, it is necessary to understand the properties (e.g., acid and alkali resistance, tensile strength, hardness, water absorption, etc.) of the laminating material, which will affect the parts used in the paper cup. Meanwhile, some techniques (e.g., FTIR, MALDI, SEM, etc.) are used to understand the microscopic structure. </p>
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             <p class="description">Since the lignin we use is not refined from trees, we will examine the film made between natural materials and our gene transferred yeast. The result can help us understand the properties impacted due to the differences between structures then can be applied on further product improvement. </p>
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            <p class="first">Device (production line) </p>
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             <p class="description">In order to use lignin on the lamination technology, the properties required for laminating are studied in the model. However, in the production of paper cups, it is impossible that only relying on the production in the laboratory. To solve the problem, we simulate a set of production lines for lignin film by researching lignin properties and visiting laminating industry. </p>
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Latest revision as of 08:48, 1 December 2018

MODELING


  In the future, there will be fewer and fewer petrochemical resources on the planet, but plastic waste are keeping accumulating. When things change, what should we do? To solve the problem, we use LIGGREEN. We hope to establish a new production line for laminating paper products using biological materials. First, we use natural enzymes to synthesize the material. Through the synthetic reaction of enzymes and monolignols, we ensure our LIGGREEN structure is similar to natural compounds. This allows us to create a biological laminate which will not require petrochemicals and high energy consumption. Our modeling is mainly divided into two parts to prove the feasibility of our project: binding model and polymer model.


Binding model

  In our experiment, coniferyl alcohol would form resonance structure after dehydrogenation, these resonance structures would form dimers (β-5, β-O-4, β-β). These reactions are catalytic by the enzymes and the addition of water.
Modeling: we decided to confirm the feasibility of the reaction through Gibbs free energy calculation. (Calculation method using Spartan 16)

Figure1: Activation energy diagram when reaction is spontaneous.

Polymer model

  We produce three enzymes, Px16, Px18 and Lac1. LIGGREEN is produced by coniferyl alcohol and enzymes. The goal of Polymer model is to estimate the polymerization between coniferyl alcohol and enzymes.
Modeling: We use Flory-Stockmayer theory to estimate the polymerization. Through the theory, we can control some conditions to do the oligomerization and let LIGGREEN be more biodegradation and chain-like.