Difference between revisions of "Team:CCU Taiwan/Safety"
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a> | ||
| + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements"><li class="list" id="home5">Achievements</li></a> | ||
</ul> | </ul> | ||
</li> | </li> | ||
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a> | ||
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/ | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Integrate"><li class="list" id="human_practice5">Integrated HP</li></a> |
</ul> | </ul> | ||
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<div class="content"> | <div class="content"> | ||
<br><br> | <br><br> | ||
| − | <p class="description">   | + | <p class="description">  The escaping of genetically modified organisms from labs is a serious problem since it will bring up unpredictable impacts to our ecosystem. We take biosafety very seriously when we design every parts of our experiment and the design of our production line. |
| + | Our design of our production line insure that there's no genetically modified yeasts (also non-genetically modified yeasts) could survived and pass the whole production line and leak into the environment or residue on our products. | ||
| + | We also dealt cautiously with wastes produced from the experiment and participated in safety training, to minimum the chance of causing biohazard pollution, or simply hurt ourselves. | ||
</p> | </p> | ||
<p class="first" id="ca1">Filtering system</p> | <p class="first" id="ca1">Filtering system</p> | ||
| − | <p class="description">  The average size of the <I>P. pastoris</I> | + | <p class="description">  The average size of the <I>P. pastoris</I> is about 4–6 μm (Gmeiner, C. et al. 2015), we use the MCE filter to separate the yeasts from leaking out the fermentation tank. |
</p> | </p> | ||
<p class="first" id="ca2">Heating system</p> | <p class="first" id="ca2">Heating system</p> | ||
| − | <p class="description">  According to the literature (Martínez, D. et al. 2015), heating at 70 °C for 1 day | + | <p class="description">  According to the literature (Martínez, D. et al. 2015), heating at 70 °C for 1 day or incubate at 30 °C for 48 hr, is sufficient to kill all the yeasts. |
| − | + | The minimum operating temperature of extrusion granulation will be over 110 °C. Therefore, no yeast could survived in the production line. | |
</p> | </p> | ||
| − | <p class="description">Following | + | <p class="description">Following are our heating tests:</p> |
<div id="Safety1" class="polaroid" style="display:inline-block"> | <div id="Safety1" class="polaroid" style="display:inline-block"> | ||
<img src="https://static.igem.org/mediawiki/2018/e/e8/T--CCU_Taiwan--CCUsafety.jpg" width="100%"> | <img src="https://static.igem.org/mediawiki/2018/e/e8/T--CCU_Taiwan--CCUsafety.jpg" width="100%"> | ||
<div class="container"> | <div class="container"> | ||
| − | <p>Figure1: <I>P. pastoris</I> heated at 30 °C for 1 day , and | + | <p>Figure1: <I>P. pastoris</I> yeast heated at 30 °C for 1 day , and incubated at 30 °C for 48 hr.<br>(left: 0.005, right: 0.05)</p> |
</div> | </div> | ||
</div> | </div> | ||
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<img src="https://static.igem.org/mediawiki/2018/4/4c/T--CCU_Taiwan--safety2.png" width="100%"> | <img src="https://static.igem.org/mediawiki/2018/4/4c/T--CCU_Taiwan--safety2.png" width="100%"> | ||
<div class="container"> | <div class="container"> | ||
| − | <p>Figure2: <I>P. pastoris</I> heated at 50 °C for 1 day , and | + | <p>Figure2: <I>P. pastoris</I> yeast heated at 50 °C for 1 day , and incubated at 30 °C for 48 hr.</p> |
</div> | </div> | ||
</div> | </div> | ||
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<img src="https://static.igem.org/mediawiki/2018/1/1e/T--CCU_Taiwan--safety3.png" width="100%"> | <img src="https://static.igem.org/mediawiki/2018/1/1e/T--CCU_Taiwan--safety3.png" width="100%"> | ||
<div class="container"> | <div class="container"> | ||
| − | <p>Figure3: <I>P. pastoris</I> heated at 70 °C for 1 day , and incubate at 30 °C for 48 hr.</p> | + | <p>Figure3: <I>P. pastoris</I> yeast heated at 70 °C for 1 day , and incubate at 30 °C for 48 hr.</p> |
</div> | </div> | ||
</div><br><br> | </div><br><br> | ||
| − | <p class="description">  We found that our <I>P. pastoris</I> | + | <p class="description">  We found that our <I>P. pastoris</I> diden't survive after were heated at 50 °C for 30 minutes. Which proof that the heat our production line generated could kill <I>P. pastoris</I> which pass the filter.</p> |
<br> | <br> | ||
Latest revision as of 08:50, 1 December 2018
SAFETY
The escaping of genetically modified organisms from labs is a serious problem since it will bring up unpredictable impacts to our ecosystem. We take biosafety very seriously when we design every parts of our experiment and the design of our production line. Our design of our production line insure that there's no genetically modified yeasts (also non-genetically modified yeasts) could survived and pass the whole production line and leak into the environment or residue on our products. We also dealt cautiously with wastes produced from the experiment and participated in safety training, to minimum the chance of causing biohazard pollution, or simply hurt ourselves.
Filtering system
The average size of the P. pastoris is about 4–6 μm (Gmeiner, C. et al. 2015), we use the MCE filter to separate the yeasts from leaking out the fermentation tank.
Heating system
According to the literature (Martínez, D. et al. 2015), heating at 70 °C for 1 day or incubate at 30 °C for 48 hr, is sufficient to kill all the yeasts. The minimum operating temperature of extrusion granulation will be over 110 °C. Therefore, no yeast could survived in the production line.
Following are our heating tests:
Figure1: P. pastoris yeast heated at 30 °C for 1 day , and incubated at 30 °C for 48 hr.
(left: 0.005, right: 0.05)
Figure2: P. pastoris yeast heated at 50 °C for 1 day , and incubated at 30 °C for 48 hr.
Figure3: P. pastoris yeast heated at 70 °C for 1 day , and incubate at 30 °C for 48 hr.
We found that our P. pastoris diden't survive after were heated at 50 °C for 30 minutes. Which proof that the heat our production line generated could kill P. pastoris which pass the filter.
Reference
Gmeiner, C., Saadati, A., Maresch, D., Krasteva, S., Frank, M., Altmann, F., … Spadiut, O. (2015). Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase. Microbial Cell Factories, 14(1). doi:10.1186/s12934-014-0183-3
Bacher, G., Szymanski, W. W., Kaufman, S. L., Zöllner, P., Blaas, D., & Allmaier, G. (2001). Charge-reduced nano electrospray ionization combined with differential mobility analysis of peptides, proteins, glycoproteins, noncovalent protein complexes and viruses. Journal of Mass Spectrometry, 36(9), 1038–1052.doi:10.1002/jms.208
3. Martínez, D., Menéndez, C., Echemendia, F. M., Hernández, L., Sobrino, A., & Trujillo, L. E. (2015). Kinetics of sucrose hydrolysis by immobilized recombinant Pichia pastoris cells in a batch reactors. J Microb Biochem Technol, 7, 294-6.