Difference between revisions of "Team:CCU Taiwan/Safety"

 
(34 intermediate revisions by 6 users not shown)
Line 1: Line 1:
−
{{Template:CCU_Taiwan/css}}
+
{{CCU_Taiwan/css}}
 +
 
 
<html>
 
<html>
 +
 +
<head>
 +
 +
<meta name="viewport" content="width=device-width, initial-scale=1">
 
<script>
 
<script>
 
$(document).ready(function(){
 
$(document).ready(function(){
 
 
−
$("#Home").hover(function(){
+
$("#Home").hover(function(e){
−
$("#sub_home").stop(true,false,true).slideToggle(300).css("opacity","0.8");
+
$("#sub_home").stop(true,false,true).slideToggle(300);
−
});
+
  });
−
+
 
 
$("#Project").hover(function(){
 
$("#Project").hover(function(){
−
$("#sub_project").stop(true,false,true).slideToggle(300).css("opacity","0.8");
+
$("#sub_project").stop(true,false,true).slideToggle(300);
 
});
 
});
 
 
 
$("#Parts").hover(function(){
 
$("#Parts").hover(function(){
−
$("#sub_parts").stop(true,false,true).slideToggle(300).css("opacity","0.8");
+
$("#sub_parts").stop(true,false,true).slideToggle(300);
 
});
 
});
 
 
−
$("#Modeling").hover(function(){
+
$("#Modeling").hover(function(){  
−
$("#sub_modeling").stop(true,false,true).slideToggle(300).css("opacity","0.8");
+
$("#sub_modeling").stop(true,false,true).slideToggle(300);
 
});
 
});
 
 
 
$("#Drylab").hover(function(){
 
$("#Drylab").hover(function(){
−
$("#sub_drylab").stop(true,false,true).slideToggle(300).css("opacity","0.8");
+
$("#sub_drylab").stop(true,false,true).slideToggle(300);
 
});
 
});
 
 
 
$("#Human_Practice").hover(function(){
 
$("#Human_Practice").hover(function(){
−
$("#sub_human_practice").stop(true,false,true).slideToggle(300).css("opacity","0.8");
+
$("#sub_human_practice").stop(true,false,true).slideToggle(300);
 
});
 
});
 
 
 
$("#Notebook").hover(function(){
 
$("#Notebook").hover(function(){
−
$("#sub_notebook").stop(true,false,true).slideToggle(300).css("opacity","0.8");
+
$("#sub_notebook").stop(true,false,true).slideToggle(300);
 
});
 
});
  
Line 35: Line 40:
 
     $(this).css("background","black")
 
     $(this).css("background","black")
 
},function(){
 
},function(){
−
     $(this).css("background","#F96784")
+
     $(this).css("background","#31a97e")
 
   });
 
   });
 +
});
  
−
$("#home1").hover(function(){
+
// The function actually applying the offset
−
$("#home_img").css('display', 'block');
+
function offsetAnchor() {
−
$("#home_img").animate({ opacity: 0 }, 0);
+
  if (location.hash.length !== 0) {
−
$("#home_img").stop(true,false,true).animate({opacity: 1,left:'25vw'},800, 'swing')
+
    window.scrollTo(window.scrollX, window.scrollY - 90);
−
$("#home_word").stop(true,false,true).delay(750).fadeIn(700)
+
  }
−
},function(){
+
}
−
$("#home_img").stop(true,false,true).css("display","none").css("left","50vw");
+
 
−
$("#home_word").fadeOut(0)
+
// Captures click events of all <a> elements with href starting with #
−
});
+
$(document).on('click', 'a[href^="#"]', function(event) {
−
});
+
  // Click events are captured before hashchanges. Timeout
 +
  // causes offsetAnchor to be called after the page jump.
 +
  window.setTimeout(function() {
 +
    offsetAnchor();
 +
  }, 0);
 +
});
 +
 
 +
// Set the offset when entering page with hash present in the url
 +
window.setTimeout(offsetAnchor, 0);
 +
 +
$(document).scroll(function () { // remove "$"
 +
    var y = $(this).scrollTop();       
 +
    if (y > 300) {
 +
        $('.indicator').fadeIn();
 +
    }
 +
    else {
 +
        $('.indicator').fadeOut();
 +
    }
 +
});
 +
 
</script>
 
</script>
 +
 +
</head>
 +
 
<body>
 
<body>
 
   <header>
 
   <header>
−
         <nav class="navbar navbar-default navbar-inverse navbar-fixed-top">
+
         <nav>
 
             <div class="container">
 
             <div class="container">
−
                 <ul>
+
                 <ul class="front">
 +
 
                     <div id="home_button" style="cursor:pointer;"  onclick="location.href=
 
                     <div id="home_button" style="cursor:pointer;"  onclick="location.href=
 
'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div>
 
'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div>
−
                     <li class="title" id="Home"><a>Home</a>
+
 
−
                         <ul class="sub" id="sub_home">
+
                     <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a>
−
                             <li class="list" id="home1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Team">Team</a></li>
+
                         <ul class="sub" id="sub_home" style="cursor:default;">
−
<li class="list" id="home2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions">Attributions</a></li>
+
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a>
−
<li class="list" id="home3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Judge">For Judge</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a>
−
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a>
−
<div id="home_img">
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a>
−
<img src="https://static.igem.org/mediawiki/2018/5/57/T--CCU_Taiwan--home.png"></img>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements"><li class="list" id="home5">Achievements</li></a>
−
</div>
+
−
<div id="home_word">
+
−
<p>There's a home for us, always.</p>
+
−
</div>
+
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
−
                     <li class="title" id="Project"><a href="#">Project</a>
+
                     <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a>
−
<ul class="sub" id="sub_project">
+
<ul class="sub" id="sub_project" style="cursor:default;">
−
    <li class="list" id="project1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Description">Description</a></li>
+
    <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a>
−
<li class="list" id="project2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Design">Design</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Applied_Design"><li class="list" id="project2">Design</li></a>
−
<li class="list" id="project3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Results">Results</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a>
−
<li class="list" id="project4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements">Achievements</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a>
−
<li class="list" id="project5"><a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab ">InterLab</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab"><li class="list" id="project5">InterLab</li></a>
−
    <li class="list" id="project6"><a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstration</a></li>
+
 
</ul>
 
</ul>
 
</li>
 
</li>
−
                     <li class="title" id="Parts"><a href="#">Parts</a>
+
                     <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a>
−
                         <ul class="sub" id="sub_parts">
+
                         <ul class="sub" id="sub_parts" style="cursor:default;">
−
    <li class="list" id="parts1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Parts">Parts</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Parts"><li class="list" id="parts1">Overview</li></a>
−
<li class="list" id="parts2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part">Basic Part</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a>
−
<li class="list" id="parts3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part">Composite Part</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a>
−
<li class="list" id="parts4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Improve">Improved Part</a></li>
+
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
−
                     <li class="title" id="Modeling"><a href="#">Modeling</a>
+
                     <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a>
−
                         <ul class="sub" id="sub_modeling">
+
                         <ul class="sub" id="sub_modeling" style="cursor:default;">
−
                            <li class="list" id="model1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Model">Model</a></li>
+
                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a>
−
                            <li class="list" id="model2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Enzyme">Enzyme Activity</a></li>
+
                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding Model</li></a>
−
<li class="list" id="model3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Monolignol">Monolignol Binding</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer Model</li></a>
−
<li class="list" id="model4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Tools">Tools</a></li>
+
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
−
                     <li class="title" id="Drylab"><a href="#">Drylab</a>
+
                     <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a>
−
                         <ul class="sub" id="sub_drylab">
+
                         <ul class="sub" id="sub_drylab" style="cursor:default;">
−
                            <li class="list" id="drylab1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan">Our Plan</a></li>
+
                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a>
−
<li class="list" id="drylab2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering">Engineering</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering"><li class="list" id="drylab2">Production Line</li></a>
−
<li class="list" id="drylab3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Safety">Safety</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Safety"><li class="list" id="drylab3">Safety</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
−
<li class="title" id="Human_Practice"><a href="#">Human Practice</a>
+
    <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a>
−
                         <ul class="sub" id="sub_human_practice">
+
                         <ul class="sub" id="sub_human_practice" style="cursor:default;">
−
                             <li class="list" id="human_practice1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices">Human Practice</a></li>
+
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Human Practice</li></a>
−
<li class="list" id="human_practice2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engagement">Engagement</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a>
−
<li class="list" id="human_practice3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship">Entrepreneurship</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a>
−
<li class="list" id="human_practice4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Business_Model">Business Model</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Integrate"><li class="list" id="human_practice5">Integrated HP</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
−
<li class="title" id="Notebook"><a href="#">Notebook</a>
+
    <li class="title" style="cursor:pointer;" id="Notebook"><img class="img_title" src="https://static.igem.org/mediawiki/2018/c/c9/T--CCU_Taiwan--notebook.png"></img><a>Notebook</a>
−
                         <ul class="sub" id="sub_notebook">
+
                         <ul class="sub" id="sub_notebook" style="cursor:default;">
−
                             <li class="list" id="notebook1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li>
+
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook"><li class="list" id="notebook1">Overview</li></a>
−
<li class="list" id="notebook2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Applied_Design">Applied Design</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Collaborations"><li class="list" id="notebook2">Collaborations</li></a>
−
<li class="list" id="notebook3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments">Experiments</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Protocols"><li class="list" id="notebook3">Protocols</li></a>
−
<li class="list" id="notebook4"><a href="https://2018.igem.org/Team:CCU_Taiwan/DATABASE">DATABASE</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments"><li class="list" id="notebook4">Experiments</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Materials"><li class="list" id="notebook5">Materials</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
Line 122: Line 146:
 
             </div>
 
             </div>
 
         </nav>
 
         </nav>
 +
 
     </header>
 
     </header>
−
</body>
+
<div class="indicator">
  
 +
<div class="pointerDrylab" id="1"><a href="#ca1">Filtering system</a></div>
 +
<div class="pointerDrylab" id="2"><a href="#ca2">Heating system</a></div>
  
−
<div class="column full_size">
+
</div>
−
 
+
−
<h1> Safety </h1>
+
−
<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
+
−
 
+
−
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
+
  
 +
<div class="backgroundDrylab">
 +
<div class="photoSafety"><h1 class="bigtitle">SAFETY<h1></div>
 +
<div class="content">
 +
<br><br>
 +
<p class="description">&emsp;&emsp;The escaping of genetically modified organisms from labs is a serious problem since it will bring up unpredictable impacts to our ecosystem. We take biosafety very seriously when we design  every parts of our experiment and the design of our production line.
 +
Our design of our production line insure that there's no genetically modified yeasts (also non-genetically modified yeasts) could survived and pass the whole production line and leak into the environment or residue on our products.
 +
We also dealt cautiously with wastes produced from the experiment and participated in safety training, to minimum the chance of causing biohazard pollution, or simply hurt ourselves.
 +
</p>
 +
<p class="first" id="ca1">Filtering system</p>
 +
<p class="description">&emsp;&emsp;The average size of the <I>P. pastoris</I> is about 4–6 μm (Gmeiner, C. et al. 2015), we use the MCE filter to separate the yeasts from leaking out the fermentation tank.
 +
</p>
 +
<p class="first" id="ca2">Heating system</p>
 +
<p class="description">&emsp;&emsp;According to the literature (Martínez, D. et al. 2015), heating at 70 °C for 1 day or incubate at 30 °C for 48 hr, is sufficient to kill all the yeasts.
 +
The minimum operating temperature of extrusion granulation will be over 110 °C. Therefore, no yeast could survived in the production line.
 +
</p>
 +
<p class="description">Following are our heating tests:</p>
 +
                <div id="Safety1" class="polaroid" style="display:inline-block">
 +
                  <img src="https://static.igem.org/mediawiki/2018/e/e8/T--CCU_Taiwan--CCUsafety.jpg" width="100%">
 +
                  <div class="container">
 +
                    <p>Figure1: <I>P. pastoris</I> yeast heated at 30 °C for 1 day , and incubated at 30 °C for 48 hr.<br>(left: 0.005, right: 0.05)</p>
 +
                  </div>
 +
                </div>
 +
                <div id="Safety2" class="polaroid" style="display:inline-block">
 +
                  <img src="https://static.igem.org/mediawiki/2018/4/4c/T--CCU_Taiwan--safety2.png" width="100%">
 +
                  <div class="container">
 +
                    <p>Figure2: <I>P. pastoris</I> yeast heated at 50 °C for 1 day , and incubated at 30 °C for 48 hr.</p>
 +
                  </div>
 +
                </div>
 +
                <div id="Safety3" class="polaroid" style="display:inline-block">
 +
                  <img src="https://static.igem.org/mediawiki/2018/1/1e/T--CCU_Taiwan--safety3.png" width="100%">
 +
                  <div class="container">
 +
                    <p>Figure3: <I>P. pastoris</I> yeast heated at 70 °C for 1 day , and incubate at 30 °C for 48 hr.</p>
 +
                  </div>
 +
                </div><br><br>
 +
<p class="description">&emsp;&emsp;We found that our <I>P. pastoris</I> diden't survive after were heated at 50 °C for 30 minutes. Which proof that the heat our production line generated could kill <I>P. pastoris</I> which pass the filter.</p>
 +
 +
<br>
 +
<p class="second"><br>Reference</p>
 +
<p class="description">
 +
Gmeiner, C., Saadati, A., Maresch, D., Krasteva, S., Frank, M., Altmann, F., … Spadiut, O. (2015). Development of a fed-batch process for a recombinant <I>Pichia pastoris</I> Δoch1 strain expressing a plant peroxidase. Microbial Cell Factories, 14(1). doi:10.1186/s12934-014-0183-3<br>
 +
<br>Bacher, G., Szymanski, W. W., Kaufman, S. L., Zöllner, P., Blaas, D., & Allmaier, G. (2001). Charge-reduced nano electrospray ionization combined with differential mobility analysis of peptides, proteins, glycoproteins, noncovalent protein complexes and viruses. Journal of Mass Spectrometry, 36(9), 1038–1052.doi:10.1002/jms.208<br>
 +
<br>3. Martínez, D., Menéndez, C., Echemendia, F. M., Hernández, L., Sobrino, A., & Trujillo, L. E. (2015). Kinetics of sucrose hydrolysis by immobilized recombinant <I>Pichia pastoris</I> cells in a batch reactors. J Microb Biochem Technol, 7, 294-6.<br>
 +
 +
</p>
 +
<br><br><br>
 
</div>
 
</div>
−
 
−
 
−
<div class="column two_thirds_size">
 
−
<h3>Safe Project Design</h3>
 
−
 
−
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
−
 
−
<ul>
 
−
<li>Choosing a non-pathogenic chassis</li>
 
−
<li>Choosing parts that will not harm humans / animals / plants</li>
 
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
−
<li>Including an "induced lethality" or "kill-switch" device</li>
 
−
</ul>
 
−
 
−
</div>
 
−
 
−
<div class="column third_size">
 
−
<h3>Safe Lab Work</h3>
 
−
 
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
−
 
−
 
−
<h3>Safe Shipment</h3>
 
−
 
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
 
</div>
 
</div>
 +
</body>
 +
<footer class="footer">
 +
            <a href="http://huitong.com.tw">
 +
              <img  id="BSlogo" src="https://static.igem.org/mediawiki/2018/0/07/T--CCU_Taiwan--CCUP1.png" align="bottom">
 +
            </a>
  
 +
            <a href="https://www.facebook.com/ccuigemteam/?ref=br_rs">
 +
            <img  id="CCUTEAMlogo" src="https://static.igem.org/mediawiki/2018/e/e3/T--CCU_Taiwan--CCUP2.png" align="bottom">
 +
            </a>
  
−
</html>
+
            <a href="http://ewww.ccu.edu.tw/">
 +
            <img  id="CCUlogo" src="https://static.igem.org/mediawiki/2018/8/89/T--CCU_Taiwan--CCUP3.png" align="bottom">
 +
            </a>
 +
</footer>

Latest revision as of 08:50, 1 December 2018

SAFETY



  The escaping of genetically modified organisms from labs is a serious problem since it will bring up unpredictable impacts to our ecosystem. We take biosafety very seriously when we design every parts of our experiment and the design of our production line. Our design of our production line insure that there's no genetically modified yeasts (also non-genetically modified yeasts) could survived and pass the whole production line and leak into the environment or residue on our products. We also dealt cautiously with wastes produced from the experiment and participated in safety training, to minimum the chance of causing biohazard pollution, or simply hurt ourselves.

Filtering system

  The average size of the P. pastoris is about 4–6 μm (Gmeiner, C. et al. 2015), we use the MCE filter to separate the yeasts from leaking out the fermentation tank.

Heating system

  According to the literature (Martínez, D. et al. 2015), heating at 70 °C for 1 day or incubate at 30 °C for 48 hr, is sufficient to kill all the yeasts. The minimum operating temperature of extrusion granulation will be over 110 °C. Therefore, no yeast could survived in the production line.

Following are our heating tests:

Figure1: P. pastoris yeast heated at 30 °C for 1 day , and incubated at 30 °C for 48 hr.
(left: 0.005, right: 0.05)

Figure2: P. pastoris yeast heated at 50 °C for 1 day , and incubated at 30 °C for 48 hr.

Figure3: P. pastoris yeast heated at 70 °C for 1 day , and incubate at 30 °C for 48 hr.



  We found that our P. pastoris diden't survive after were heated at 50 °C for 30 minutes. Which proof that the heat our production line generated could kill P. pastoris which pass the filter.



Reference

Gmeiner, C., Saadati, A., Maresch, D., Krasteva, S., Frank, M., Altmann, F., … Spadiut, O. (2015). Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase. Microbial Cell Factories, 14(1). doi:10.1186/s12934-014-0183-3

Bacher, G., Szymanski, W. W., Kaufman, S. L., Zöllner, P., Blaas, D., & Allmaier, G. (2001). Charge-reduced nano electrospray ionization combined with differential mobility analysis of peptides, proteins, glycoproteins, noncovalent protein complexes and viruses. Journal of Mass Spectrometry, 36(9), 1038–1052.doi:10.1002/jms.208

3. Martínez, D., Menéndez, C., Echemendia, F. M., Hernández, L., Sobrino, A., & Trujillo, L. E. (2015). Kinetics of sucrose hydrolysis by immobilized recombinant Pichia pastoris cells in a batch reactors. J Microb Biochem Technol, 7, 294-6.