Difference between revisions of "Team:CCU Taiwan/Notebook"

 
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  <header>
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        <nav>
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            <nav class="navbar navbar-default navbar-inverse navbar-fixed-top">
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            <div class="container">
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                <div class="container">
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                <ul class="front">
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                    <ul>
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                        <li><a href="https://2018.igem.org/Team:CCU_Taiwan">Home</a>
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                    <div id="home_button" style="cursor:pointer;"  onclick="location.href=
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                            <ul>
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'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div>
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                                <li><a href="#">our team</a></li>
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                                <li><a href="#">resourses</a></li>
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                    <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a>
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                            </ul>
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                        <ul class="sub" id="sub_home" style="cursor:default;">
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                        </li>
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a>
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                        <li><a href="#">Project</a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a>
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                            <ul>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a>
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                                <li><a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab">Interlab</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a>
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                                <li><a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements"><li class="list" id="home5">Achievements</li></a>
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                            </ul>
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                        </ul>
−
                         </li>
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                    </li>
−
                         <li><a href="#">Result</a></li>
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                    <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a>
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                         <li><a href="#">Human Practice</a></li>
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<ul class="sub" id="sub_project" style="cursor:default;">
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                        <li><a href="#">Modeling</a></li>
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    <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a>
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                         <li><a href="#">Safety</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Applied_Design"><li class="list" id="project2">Design</li></a>
−
                        <li><a href="#">Medals</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a>
−
                    </ul>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a>
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                 </div>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab"><li class="list" id="project5">InterLab</li></a>
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             </nav>
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</ul>
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         </header>
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</li>
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        <div <div class="interlab">>
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                    <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a>
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            <p class="first">Notebook</p>
+
                        <ul class="sub" id="sub_parts" style="cursor:default;">
−
            <p class="first">February</p>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Parts"><li class="list" id="parts1">Overview</li></a>
−
            <p class="description"> Molecular biology class </p>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a>
−
            <p class="description"> e-coli genomic DNA preparation </p>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a>
−
            <p class="description"> e-coli transformation </p>
+
                        </ul>
−
            <p class="first">March</p>
+
                    </li>
−
            <p class="description"> Instrument operation </p>
+
                    <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a>
−
            <p class="first">April</p>
+
                        <ul class="sub" id="sub_modeling" style="cursor:default;">
−
            <p class="description"> Project brainstorming-Product Positioning, HGS monomer proportion, </p>
+
                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a>
−
            <p class="first">May</p>
+
                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding Model</li></a>
−
            <p class="description"> Culture selection-compare Yeast, E-coli, Acetobacter aceti</p>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer Model</li></a>
−
            <p class="description"> Gene design</p>
+
                         </ul>
−
            <p class="first">June</p>
+
                    </li>
−
            <p class="description"> Interlab experiment-Calbration1,2,3</p>
+
                    <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a>
−
            <p class="first">July</p>
+
                         <ul class="sub" id="sub_drylab" style="cursor:default;">
−
            <p class="second"> 7/3</p>
+
                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a>
−
            <p class="description"> Start interlab experiment-cell measurement</p>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering"><li class="list" id="drylab2">Production Line</li></a>
−
            <p class="second"> 7/5</p>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Safety"><li class="list" id="drylab3">Safety</li></a>
−
            <p class="description"> YPD formulation </p>
+
                         </ul>
−
            <p class="second"> 7/9</p>
+
                    </li>
−
            <p class="description">Yeast(X33) culture</p>
+
    <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a>
−
            <p class="second"> 7/13</p>
+
                        <ul class="sub" id="sub_human_practice" style="cursor:default;">
−
            <p class="description"> Fundraising briefing session</p>
+
                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Human Practice</li></a>
−
            <p class="second">  7/16</p>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a>
−
            <p class="description"> communicate with NCKU(interlab & project) </p>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a>
−
            <p class="second"> 7/18</p>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a>
−
            <p class="description">communicate with BIT(project) </p>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Integrate"><li class="list" id="human_practice5">Integrated HP</li></a>
−
        </div>
+
                         </ul>
−
    </div>
+
                    </li>
 +
    <li class="title" style="cursor:pointer;" id="Notebook"><img class="img_title" src="https://static.igem.org/mediawiki/2018/c/c9/T--CCU_Taiwan--notebook.png"></img><a>Notebook</a>
 +
                        <ul class="sub" id="sub_notebook" style="cursor:default;">
 +
                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook"><li class="list" id="notebook1">Overview</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Collaborations"><li class="list" id="notebook2">Collaborations</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Protocols"><li class="list" id="notebook3">Protocols</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments"><li class="list" id="notebook4">Experiments</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Materials"><li class="list" id="notebook5">Materials</li></a>
 +
                        </ul>
 +
                    </li>
 +
                 </ul>
 +
             </div>
 +
         </nav>
 +
 
 +
    </header>
 +
<div class="indicator">
 +
 
 +
<div class="pointerNotebook" id="1"><a href="#ca1">Notebook</a></div>
 +
</div>
 +
 
 +
<div class="backgroundNotebook">
 +
<div class="photoNotebook"><h1 class="bigtitle">NOTEBOOK<h1></div>
 +
      <div class="content">
 +
<p class="first" id="ca1">Notebook</p>
 +
<p class="second">February</p>
 +
<p class="description">&emsp;&emsp;&emsp;&emsp;Molecular biology class<br>
 +
    &emsp;&emsp;&emsp;&emsp;<I>E. coli</I> genomic DNA preparation<br>
 +
    &emsp;&emsp;&emsp;&emsp;<I>E. coli</I> transformation<br>
 +
</p>
 +
<p class="second">March</p>
 +
<p class="description">&emsp;&emsp;&emsp;&emsp;Instrument operation</p>
 +
<p class="second">April</p>
 +
<p class="description">&emsp;&emsp;&emsp;&emsp;Project brainstorming: Product Positioning, Monolignol proportion</p>
 +
<p class="second">May</p>
 +
<p class="description">&emsp;&emsp;&emsp;&emsp;Culture selection: compare Yeast, <I>E. coli</I>, Acetobacter aceti<br>
 +
&emsp;&emsp;&emsp;&emsp;Gene design
 +
</p>
 +
<p class="second">June</p>
 +
<p class="description">&emsp;&emsp;&emsp;&emsp;Interlab experiment: Calibration 1, 2, 3</p>
 +
<p class="second">July</p>
 +
<p class="description">&emsp;&emsp;<strong>7/3</strong><br>
 +
&emsp;&emsp;&emsp;&emsp;Start Interlab experiment: cell measurement<br>
 +
&emsp;&emsp;<strong>7/5</strong><br>
 +
&emsp;&emsp;&emsp;&emsp;Yeast Extract–Peptone–Dextrose (YPD) formulation <br>
 +
&emsp;&emsp;<strong>7/9</strong><br>
 +
&emsp;&emsp;&emsp;&emsp;Yeast (X33) culture<br>
 +
&emsp;&emsp;<strong>7/13</strong><br>
 +
    &emsp;&emsp;&emsp;&emsp;Fundraising briefing session<br>
 +
&emsp;&emsp;<strong>7/16</strong><br>
 +
    &emsp;&emsp;&emsp;&emsp;Communicate with NCKU (Interlab & project)<br>
 +
&emsp;&emsp;<strong>7/18</strong><br>
 +
    &emsp;&emsp;&emsp;&emsp;Communicate with BIT (project)<br>
 +
</p>
 +
<p class="second">August</p>
 +
<p class="description"><strong>8/5~8/12</strong></p>
 +
<p class="description">&emsp;&emsp;&emsp;&emsp;Cell growth, sampling, and assay<br>
 +
&emsp;&emsp;&emsp;&emsp;Digest pGAPZ A(X3)/ pUCIDT_Lac1/ pUCIDT_Px16/ pUCIDT_Px18 with AgeI, EcoRI<br>
 +
&emsp;&emsp;&emsp;&emsp;Transformation pGAPZ A_Lac1/ pGAPZ A_Px16/ pGAPZ A_Px18 (15µl) into 20µl ECOS™<br>
 +
&emsp;&emsp;&emsp;&emsp;101 Competent Cells [DH5a]<br>
 +
&emsp;&emsp;&emsp;&emsp;Ligation pGAPZ A_Lac1/ pGAPZ A_Px16/ pGAPZ A_Px18<br>
 +
&emsp;&emsp;&emsp;&emsp;Digest pUCIDT_Lac1/ pUCIDT_Px16/ pUCIDT_Px18 with AgeI, EcoRI<br>
 +
</p>
 +
<p class="description"><strong>8/13~8/19</strong></p>
 +
<p class="description">&emsp;&emsp;&emsp;&emsp;Miniprep pGAPZ A_Lac1/ pGAPZ A_Px16/ pGAPZ A_Px18<br>
 +
&emsp;&emsp;&emsp;&emsp;Digest pGAPZ A(X3)/ pUCIDT_Lac1/ pUCIDT_Px16/ pUCIDT_Px18 with AgeI, EcoRI<br>
 +
&emsp;&emsp;&emsp;&emsp;Digest pGAPZ A with SalI<br>
 +
&emsp;&emsp;&emsp;&emsp;Incubate with DNA Polymerase I, Large (Klenow) Fragment remove SalI restriction site<br>
 +
&emsp;&emsp;&emsp;&emsp;Ligation pGAPZ A-SalI(pGAPZ A-1)<br>
 +
&emsp;&emsp;&emsp;&emsp;Transformation of pGAPZ A-SalI(pGAPZ A-1)<br>
 +
&emsp;&emsp;&emsp;&emsp;Minipreparation of pGAPZ A-SalI(pGAPZ A-1)
 +
</p>
 +
<p class="description"><strong>8/20~8/26</strong></p>
 +
<p class="description">&emsp;&emsp;Gel extract<br>
 +
&emsp;&emsp;&emsp;&emsp;Digestion – pGAPZ A-1+HIS4 cut with AgeI and EcoRI (x3)<br>
 +
&emsp;&emsp;&emsp;&emsp;Ligation - pGAPZ A_Lac1/ pGAPZ A_Px16/ pGAPZ A_Px18
 +
</p>
 +
<p class="description"><strong>8/27~9/2</strong></p>
 +
<p class="description">&emsp;&emsp;&emsp;&emsp;Digestion – pGAPZ A-1+HIS4 cut with AgeI and EcoRI (x3)<br>
 +
&emsp;&emsp;&emsp;&emsp;Ligation – pGAPZ A-1+HIS4 cut with AgeI and EcoRI (x3)<br><br>
 +
 
 +
&emsp;&emsp;&emsp;&emsp;Transform <br>
 +
&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;1. pGAPZ A-1 + HIS4 + Px16 <br>
 +
&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;2. pGAPZ A-1 + HIS4 + Px18<br>
 +
&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;3. pGAPZ A-1 + HIS4 + Lac1<br>
 +
&emsp;&emsp;&emsp;&emsp;Digest pGAPZ A HIS4(X2) with AgeI, EcoRI<br>
 +
&emsp;&emsp;&emsp;&emsp;pGAPZ A_Px16, pGAPZ A_Px18 , pGAPZ A_Lac1 Traditional plasmid DNA extraction<br>
 +
&emsp;&emsp;&emsp;&emsp;Yeast SMD1168 Transformation(pGAPZ A_Px16, pGAPZ A_Px18 , pGAPZ A_Lac1)
 +
</p>
 +
 
 +
<p class="second">September</p>
 +
<p class="description"><strong>9/3~9/9</strong></p>
 +
<p class="description">&emsp;&emsp;&emsp;&emsp;PCR—pGAPZ A-1 + HIS4 + Px16, Px18, Lac1<br>
 +
&emsp;&emsp;&emsp;&emsp;miniprep – pGAPZ A-1 + HIS4 + Px16, Px18<br>
 +
&emsp;&emsp;&emsp;&emsp;Transform – pGAPZ A-1 + HIS4 + Lac1<br>
 +
&emsp;&emsp;&emsp;&emsp;Digestion – pGAPZ A-1 + HIS4+lac1<br>
 +
</p>
 +
<p class="description"><strong>9/12~9/14</strong></p>
 +
<p class="description">&emsp;&emsp;&emsp;&emsp;Colony PCR gel electrophoresis check - HIS4+Px16, HIS4+Lac1<br>
 +
&emsp;&emsp;&emsp;&emsp;Culture HIS4+Px16, HIS4+Lac1<br>
 +
&emsp;&emsp;&emsp;&emsp;Colony PCR gel electrophoresis check - HIS4+Px16, HIS4+Lac1<br>
 +
&emsp;&emsp;&emsp;&emsp;Yeast SMD1168 pGAPZ A_Px16, pGAPZ A_Px18, pGAPZ A_Lac1 colony PCR <br>
 +
&emsp;&emsp;&emsp;&emsp;Plasmid miniprep - HIS4+Lac1, HIS4+Px16
 +
</p>
 +
<p class="description"><strong>9/24~9/27</strong></p>
 +
<p class="description">&emsp;&emsp;&emsp;&emsp;Culture HIS4+Lac1 x3<br>
 +
&emsp;&emsp;&emsp;&emsp;Prepare yeast competent cells (SMD1168)<br>
 +
&emsp;&emsp;&emsp;&emsp;Traditional plasmid DNA extraction Day1 <br>
 +
&emsp;&emsp;&emsp;&emsp;Yeast SMD1168 pGAPZ A_Px16, pGAPZ A_Px18, pGAPZ A_Lac1 colony PCR  <br>
 +
&emsp;&emsp;&emsp;&emsp;Yeast SMD1168 pGAPZ A_Px16, pGAPZ A_Px18, pGAPZ A_Lac1 Western blot  <br>
 +
&emsp;&emsp;&emsp;&emsp;Prepare YPD plates<br>
 +
&emsp;&emsp;&emsp;&emsp;Prepare the amino acids of SD plates<br>
 +
&emsp;&emsp;&emsp;&emsp;Traditional plasmid DNA extraction Day2<br>
 +
&emsp;&emsp;&emsp;&emsp;Plasmids contain HIS4 cut with SalI<br>
 +
&emsp;&emsp;&emsp;&emsp;Yeast transformation - Px16, Px18, Lac1 (plasmids contain HIS4)
 +
</p>
 +
 
 +
<p class="second">October</p>
 +
<p class="description"><strong>10/8~10/11</strong></p>
 +
<p class="description">&emsp;&emsp;&emsp;&emsp;Prepare yeast competent cells (SMD1168)<br>
 +
&emsp;&emsp;&emsp;&emsp;Colony PCR (HIS4+Px16)<br>
 +
&emsp;&emsp;&emsp;&emsp;Safety (30℃, 50℃, 70℃)<br>
 +
&emsp;&emsp;&emsp;&emsp;Yeast transformation - Px16, Lac1 (plasmids contain HIS4)<br><br>
 +
&emsp;&emsp;&emsp;&emsp;Yeast SMD1168 pGAPZ A_Px16, pGAPZ A_Px18, pGAPZ A_Lac1 Western blot  <br>
 +
<br><br>
 +
</p>
 +
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 +
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Latest revision as of 08:52, 1 December 2018

NOTEBOOK

Notebook

February

    Molecular biology class
    E. coli genomic DNA preparation
    E. coli transformation

March

    Instrument operation

April

    Project brainstorming: Product Positioning, Monolignol proportion

May

    Culture selection: compare Yeast, E. coli, Acetobacter aceti
    Gene design

June

    Interlab experiment: Calibration 1, 2, 3

July

  7/3
    Start Interlab experiment: cell measurement
  7/5
    Yeast Extract–Peptone–Dextrose (YPD) formulation
  7/9
    Yeast (X33) culture
  7/13
    Fundraising briefing session
  7/16
    Communicate with NCKU (Interlab & project)
  7/18
    Communicate with BIT (project)

August

8/5~8/12

    Cell growth, sampling, and assay
    Digest pGAPZ A(X3)/ pUCIDT_Lac1/ pUCIDT_Px16/ pUCIDT_Px18 with AgeI, EcoRI
    Transformation pGAPZ A_Lac1/ pGAPZ A_Px16/ pGAPZ A_Px18 (15µl) into 20µl ECOS™
    101 Competent Cells [DH5a]
    Ligation pGAPZ A_Lac1/ pGAPZ A_Px16/ pGAPZ A_Px18
    Digest pUCIDT_Lac1/ pUCIDT_Px16/ pUCIDT_Px18 with AgeI, EcoRI

8/13~8/19

    Miniprep pGAPZ A_Lac1/ pGAPZ A_Px16/ pGAPZ A_Px18
    Digest pGAPZ A(X3)/ pUCIDT_Lac1/ pUCIDT_Px16/ pUCIDT_Px18 with AgeI, EcoRI
    Digest pGAPZ A with SalI
    Incubate with DNA Polymerase I, Large (Klenow) Fragment remove SalI restriction site
    Ligation pGAPZ A-SalI(pGAPZ A-1)
    Transformation of pGAPZ A-SalI(pGAPZ A-1)
    Minipreparation of pGAPZ A-SalI(pGAPZ A-1)

8/20~8/26

  Gel extract
    Digestion – pGAPZ A-1+HIS4 cut with AgeI and EcoRI (x3)
    Ligation - pGAPZ A_Lac1/ pGAPZ A_Px16/ pGAPZ A_Px18

8/27~9/2

    Digestion – pGAPZ A-1+HIS4 cut with AgeI and EcoRI (x3)
    Ligation – pGAPZ A-1+HIS4 cut with AgeI and EcoRI (x3)

    Transform
      1. pGAPZ A-1 + HIS4 + Px16
      2. pGAPZ A-1 + HIS4 + Px18
      3. pGAPZ A-1 + HIS4 + Lac1
    Digest pGAPZ A HIS4(X2) with AgeI, EcoRI
    pGAPZ A_Px16, pGAPZ A_Px18 , pGAPZ A_Lac1 Traditional plasmid DNA extraction
    Yeast SMD1168 Transformation(pGAPZ A_Px16, pGAPZ A_Px18 , pGAPZ A_Lac1)

September

9/3~9/9

    PCR—pGAPZ A-1 + HIS4 + Px16, Px18, Lac1
    miniprep – pGAPZ A-1 + HIS4 + Px16, Px18
    Transform – pGAPZ A-1 + HIS4 + Lac1
    Digestion – pGAPZ A-1 + HIS4+lac1

9/12~9/14

    Colony PCR gel electrophoresis check - HIS4+Px16, HIS4+Lac1
    Culture HIS4+Px16, HIS4+Lac1
    Colony PCR gel electrophoresis check - HIS4+Px16, HIS4+Lac1
    Yeast SMD1168 pGAPZ A_Px16, pGAPZ A_Px18, pGAPZ A_Lac1 colony PCR
    Plasmid miniprep - HIS4+Lac1, HIS4+Px16

9/24~9/27

    Culture HIS4+Lac1 x3
    Prepare yeast competent cells (SMD1168)
    Traditional plasmid DNA extraction Day1
    Yeast SMD1168 pGAPZ A_Px16, pGAPZ A_Px18, pGAPZ A_Lac1 colony PCR
    Yeast SMD1168 pGAPZ A_Px16, pGAPZ A_Px18, pGAPZ A_Lac1 Western blot
    Prepare YPD plates
    Prepare the amino acids of SD plates
    Traditional plasmid DNA extraction Day2
    Plasmids contain HIS4 cut with SalI
    Yeast transformation - Px16, Px18, Lac1 (plasmids contain HIS4)

October

10/8~10/11

    Prepare yeast competent cells (SMD1168)
    Colony PCR (HIS4+Px16)
    Safety (30℃, 50℃, 70℃)
    Yeast transformation - Px16, Lac1 (plasmids contain HIS4)

    Yeast SMD1168 pGAPZ A_Px16, pGAPZ A_Px18, pGAPZ A_Lac1 Western blot