Difference between revisions of "Team:CCU Taiwan/Experiments"
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'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div> | 'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div> | ||
| − | <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a | + | <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a> |
<ul class="sub" id="sub_home" style="cursor:default;"> | <ul class="sub" id="sub_home" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a> | ||
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id=" | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a> |
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id=" | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a> |
| + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements"><li class="list" id="home5">Achievements</li></a> | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a | + | <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a> |
<ul class="sub" id="sub_project" style="cursor:default;"> | <ul class="sub" id="sub_project" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a> | ||
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a> | ||
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab "><li class="list" id="project5">InterLab</li></a> | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab"><li class="list" id="project5">InterLab</li></a> |
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a | + | <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a> |
<ul class="sub" id="sub_parts" style="cursor:default;"> | <ul class="sub" id="sub_parts" style="cursor:default;"> | ||
| + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Parts"><li class="list" id="parts1">Overview</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a> | ||
| − | |||
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a | + | <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a> |
<ul class="sub" id="sub_modeling" style="cursor:default;"> | <ul class="sub" id="sub_modeling" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a> | ||
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding</li></a> | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding Model</li></a> |
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer</li></a> | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer Model</li></a> |
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a | + | <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a> |
<ul class="sub" id="sub_drylab" style="cursor:default;"> | <ul class="sub" id="sub_drylab" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a | + | <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a> |
<ul class="sub" id="sub_human_practice" style="cursor:default;"> | <ul class="sub" id="sub_human_practice" style="cursor:default;"> | ||
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1"> | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Human Practice</li></a> |
<a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a> | ||
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/ | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Integrate"><li class="list" id="human_practice5">Integrated HP</li></a> |
</ul> | </ul> | ||
</li> | </li> | ||
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</header> | </header> | ||
| + | <div class="indicator"> | ||
| + | |||
| + | <div class="pointerProject" id="2"><a href="#ca1">Notebooks</a></div> | ||
| + | </div> | ||
<div class="backgroundNotebook"> | <div class="backgroundNotebook"> | ||
<div class="photoExperiments"><h1 class="bigtitle">EXPERIMENTS<h1></div> | <div class="photoExperiments"><h1 class="bigtitle">EXPERIMENTS<h1></div> | ||
<div class="content"> | <div class="content"> | ||
| − | <iframe src="https://static.igem.org/mediawiki/2018/ | + | <p class="first" id="ca1">Laboratory notebooks</p> |
| + | <p class="description">Our project aims to produce new material— LIGGREEN. In our experiment, we select <I>P. pastoris</I> as our strain to secrete enzymes which helps the polymerization of coniferyl alcohol. Besides, we divide into two groups to conduct experiment— gene sequence with or without Zeocin™.<br><br> | ||
| + | Here are the common steps of our experiment：<br>1. Clone our vector in <I>E.coli</I> <br>2. Minipreparate our vector and purified from <I>E.coli</I> <br>3. Transform the linear vector into <I>P. pastoris</I> <br>4. Select <I>P. pastoris</I> strains <br> 5. Conduct western blot to detect our target enzyme <br>6. Purified target enzyme.</p> | ||
| + | <iframe src="https://static.igem.org/mediawiki/2018/6/6d/T--CCU_Taiwan--.expppp.pdf" height="800" width="950" style="margin-left:20vw;"></iframe> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | <br><br><br> | ||
</body> | </body> | ||
Latest revision as of 08:53, 1 December 2018
EXPERIMENTS
Laboratory notebooks
Our project aims to produce new material— LIGGREEN. In our experiment, we select P. pastoris as our strain to secrete enzymes which helps the polymerization of coniferyl alcohol. Besides, we divide into two groups to conduct experiment— gene sequence with or without Zeocin™.
Here are the common steps of our experiment：
1. Clone our vector in E.coli
2. Minipreparate our vector and purified from E.coli
3. Transform the linear vector into P. pastoris
4. Select P. pastoris strains
5. Conduct western blot to detect our target enzyme
6. Purified target enzyme.