Difference between revisions of "Team:CCU Taiwan/Experiments"

 
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements"><li class="list" id="home5">Achievements</li></a>
 
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a>
 
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Intergrate"><li class="list" id="human_practice5">Intergrated HP</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Integrate"><li class="list" id="human_practice5">Integrated HP</li></a>
 
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<div class="pointerProject" id="2"><a href="#ca1">Notebooks</a></div>
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<div class="photoExperiments"><h1 class="bigtitle">EXPERIMENTS<h1></div>
 
<div class="photoExperiments"><h1 class="bigtitle">EXPERIMENTS<h1></div>
 
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<p class="description">Our project aims to produce new material— <I>Liggreen</I>. In our experiment, we select <I>P.pastoris</I> as our strain to secrete enzymes which helps the polymerization of coniferyl alcohol. Besides, we divide into two groups to conduct experiment— gene sequence with or without Zeocin.<br><br>
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        <p class="first" id="ca1">Laboratory notebooks</p>
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Here are the common steps of our experiment：<br>1. Binding our gene sequence by the transformation of E.coli <br>2. Extracting and purifying DNA purification from E.coli <br>3. Transforming the synthetic DNA into P.pastoris <br>4. Conducting western blot to detect our target enzyme <br>5. Purified target enzyme</p>
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<p class="description">Our project aims to produce new material— LIGGREEN. In our experiment, we select <I>P. pastoris</I> as our strain to secrete enzymes which helps the polymerization of coniferyl alcohol. Besides, we divide into two groups to conduct experiment— gene sequence with or without Zeocin™.<br><br>
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<iframe src="https://static.igem.org/mediawiki/2018/5/5e/T--CCU_Taiwan--experimentv1.pdf"  height="800"  width="950" style="margin-left:20vw;"></iframe>
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Here are the common steps of our experiment：<br>1. Clone our vector in <I>E.coli</I> <br>2. Minipreparate our vector and purified from <I>E.coli</I> <br>3. Transform the linear vector into <I>P. pastoris</I> <br>4. Select  <I>P. pastoris</I>  strains <br> 5. Conduct western blot to detect our target enzyme <br>6. Purified target enzyme.</p>
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<iframe src="https://static.igem.org/mediawiki/2018/6/6d/T--CCU_Taiwan--.expppp.pdf"  height="800"  width="950" style="margin-left:20vw;"></iframe>
 
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Latest revision as of 08:53, 1 December 2018

EXPERIMENTS

Laboratory notebooks

Our project aims to produce new material— LIGGREEN. In our experiment, we select P. pastoris as our strain to secrete enzymes which helps the polymerization of coniferyl alcohol. Besides, we divide into two groups to conduct experiment— gene sequence with or without Zeocin™.

Here are the common steps of our experiment：
1. Clone our vector in E.coli
2. Minipreparate our vector and purified from E.coli
3. Transform the linear vector into P. pastoris
4. Select P. pastoris strains
5. Conduct western blot to detect our target enzyme
6. Purified target enzyme.