Difference between revisions of "Team:CCU Taiwan/Experiments"

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<div class="column full_size">
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<head>
  
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<h1>Experiments</h1>
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<meta name="viewport" content="width=device-width, initial-scale=1">
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<p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
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Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.  
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</p>
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<div class="column two_thirds_size">
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<h3>What should this page contain?</h3>
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<ul>
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<li> Protocols </li>
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$(document).scroll(function () { // remove "$"
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<li> Experiments </li>
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<li> Documentation of the development of your project </li>
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</head>
  
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<body>
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<div class="highlight decoration_A_full">
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  <header>
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<h3>Inspiration</h3>
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        <nav>
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<ul>
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            <div class="container">
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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                <ul class="front">
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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                    <div id="home_button" style="cursor:pointer;"  onclick="location.href=
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</ul>
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'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div>
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</div>
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</div>
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                    <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a>
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                        <ul class="sub" id="sub_home" style="cursor:default;">
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements"><li class="list" id="home5">Achievements</li></a>
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                        </ul>
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                    </li>
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                    <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a>
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<ul class="sub" id="sub_project" style="cursor:default;">
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    <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Applied_Design"><li class="list" id="project2">Design</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab"><li class="list" id="project5">InterLab</li></a>
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</ul>
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</li>
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                    <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a>
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                        <ul class="sub" id="sub_parts" style="cursor:default;">
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Parts"><li class="list" id="parts1">Overview</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a>
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                        </ul>
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                    </li>
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                    <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a>
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                        <ul class="sub" id="sub_modeling" style="cursor:default;">
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a>
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding Model</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer Model</li></a>
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                        </ul>
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                    </li>
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                    <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a>
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                        <ul class="sub" id="sub_drylab" style="cursor:default;">
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering"><li class="list" id="drylab2">Production Line</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Safety"><li class="list" id="drylab3">Safety</li></a>
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                        </ul>
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                    </li>
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    <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a>
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                        <ul class="sub" id="sub_human_practice" style="cursor:default;">
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Human Practice</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Integrate"><li class="list" id="human_practice5">Integrated HP</li></a>
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                        </ul>
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                    </li>
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    <li class="title" style="cursor:pointer;" id="Notebook"><img class="img_title" src="https://static.igem.org/mediawiki/2018/c/c9/T--CCU_Taiwan--notebook.png"></img><a>Notebook</a>
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                        <ul class="sub" id="sub_notebook" style="cursor:default;">
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook"><li class="list" id="notebook1">Overview</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Collaborations"><li class="list" id="notebook2">Collaborations</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Protocols"><li class="list" id="notebook3">Protocols</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments"><li class="list" id="notebook4">Experiments</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Materials"><li class="list" id="notebook5">Materials</li></a>
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                        </ul>
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                    </li>
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                </ul>
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            </div>
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        </nav>
  
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<div class="pointerProject" id="2"><a href="#ca1">Notebooks</a></div>
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<div class="photoExperiments"><h1 class="bigtitle">EXPERIMENTS<h1></div>
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      <div class="content">
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        <p class="first" id="ca1">Laboratory notebooks</p>
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<p class="description">Our project aims to produce new material— LIGGREEN. In our experiment, we select <I>P. pastoris</I> as our strain to secrete enzymes which helps the polymerization of coniferyl alcohol. Besides, we divide into two groups to conduct experiment— gene sequence with or without Zeocin™.<br><br>
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Here are the common steps of our experiment：<br>1. Clone our vector in <I>E.coli</I> <br>2. Minipreparate our vector and purified from <I>E.coli</I> <br>3. Transform the linear vector into  <I>P. pastoris</I> <br>4. Select  <I>P. pastoris</I>  strains <br> 5. Conduct western blot to detect our target enzyme  <br>6.  Purified target enzyme.</p>
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<iframe src="https://static.igem.org/mediawiki/2018/6/6d/T--CCU_Taiwan--.expppp.pdf"  height="800"  width="950" style="margin-left:20vw;"></iframe>
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Latest revision as of 08:53, 1 December 2018

EXPERIMENTS

Laboratory notebooks

Our project aims to produce new material— LIGGREEN. In our experiment, we select P. pastoris as our strain to secrete enzymes which helps the polymerization of coniferyl alcohol. Besides, we divide into two groups to conduct experiment— gene sequence with or without Zeocin™.

Here are the common steps of our experiment：
1. Clone our vector in E.coli
2. Minipreparate our vector and purified from E.coli
3. Transform the linear vector into P. pastoris
4. Select P. pastoris strains
5. Conduct western blot to detect our target enzyme
6. Purified target enzyme.