Difference between revisions of "Team:CCU Taiwan/Experiments"

 
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments"><li class="list" id="notebook4">Experiments</li></a>
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<h1>Experiments</h1>
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<p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
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        <p class="first" id="ca1">Laboratory notebooks</p>
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<p class="description">Our project aims to produce new material— LIGGREEN. In our experiment, we select <I>P. pastoris</I> as our strain to secrete enzymes which helps the polymerization of coniferyl alcohol. Besides, we divide into two groups to conduct experiment— gene sequence with or without Zeocin™.<br><br>
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Here are the common steps of our experiment：<br>1. Clone our vector in <I>E.coli</I> <br>2. Minipreparate our vector and purified from <I>E.coli</I> <br>3. Transform the linear vector into  <I>P. pastoris</I> <br>4. Select  <I>P. pastoris</I>  strains <br> 5. Conduct western blot to detect our target enzyme  <br>6.  Purified target enzyme.</p>
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<iframe src="https://static.igem.org/mediawiki/2018/6/6d/T--CCU_Taiwan--.expppp.pdf"  height="800"  width="950" style="margin-left:20vw;"></iframe>
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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Latest revision as of 08:53, 1 December 2018

EXPERIMENTS

Laboratory notebooks

Our project aims to produce new material— LIGGREEN. In our experiment, we select P. pastoris as our strain to secrete enzymes which helps the polymerization of coniferyl alcohol. Besides, we divide into two groups to conduct experiment— gene sequence with or without Zeocin™.

Here are the common steps of our experiment：
1. Clone our vector in E.coli
2. Minipreparate our vector and purified from E.coli
3. Transform the linear vector into P. pastoris
4. Select P. pastoris strains
5. Conduct western blot to detect our target enzyme
6. Purified target enzyme.