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'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div> | 'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div> | ||
− | <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a | + | <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a> |
<ul class="sub" id="sub_home" style="cursor:default;"> | <ul class="sub" id="sub_home" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a> | ||
− | <a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id=" | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a> |
− | <a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id=" | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a> |
+ | <a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements"><li class="list" id="home5">Achievements</li></a> | ||
</ul> | </ul> | ||
</li> | </li> | ||
− | <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a | + | <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a> |
<ul class="sub" id="sub_project" style="cursor:default;"> | <ul class="sub" id="sub_project" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a> | ||
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a> | ||
− | <a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab "><li class="list" id="project5">InterLab</li></a> | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab"><li class="list" id="project5">InterLab</li></a> |
</ul> | </ul> | ||
</li> | </li> | ||
− | <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a | + | <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a> |
<ul class="sub" id="sub_parts" style="cursor:default;"> | <ul class="sub" id="sub_parts" style="cursor:default;"> | ||
+ | <a href="https://2018.igem.org/Team:CCU_Taiwan/Parts"><li class="list" id="parts1">Overview</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a> | ||
− | |||
</ul> | </ul> | ||
</li> | </li> | ||
− | <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a | + | <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a> |
<ul class="sub" id="sub_modeling" style="cursor:default;"> | <ul class="sub" id="sub_modeling" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a> | ||
− | <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding</li></a> | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding Model</li></a> |
− | <a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer</li></a> | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer Model</li></a> |
</ul> | </ul> | ||
</li> | </li> | ||
− | <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a | + | <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a> |
<ul class="sub" id="sub_drylab" style="cursor:default;"> | <ul class="sub" id="sub_drylab" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
− | <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a | + | <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a> |
<ul class="sub" id="sub_human_practice" style="cursor:default;"> | <ul class="sub" id="sub_human_practice" style="cursor:default;"> | ||
− | <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1"> | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Human Practice</li></a> |
<a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a> | ||
− | <a href="https://2018.igem.org/Team:CCU_Taiwan/ | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Integrate"><li class="list" id="human_practice5">Integrated HP</li></a> |
</ul> | </ul> | ||
</li> | </li> | ||
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</header> | </header> | ||
+ | |||
+ | |||
+ | <div class="indicator"> | ||
+ | |||
+ | <div class="pointerNotebook" id="1"><a href="#ca1">Antibiotics</a></div> | ||
+ | <div class="pointerNotebook" id="2"><a href="#ca2">Antibody</a></div> | ||
+ | <div class="pointerNotebook" id="3"><a href="#ca3">Enzyme</a></div> | ||
+ | <div class="pointerNotebook" id="4"><a href="#ca4">Equipment</a></div> | ||
+ | <div class="pointerNotebook" id="5"><a href="#ca5">Kits</a></div> | ||
+ | <div class="pointerNotebook" id="4"><a href="#ca6">Markers</a></div> | ||
+ | <div class="pointerNotebook" id="5"><a href="#ca7">Media</a></div> | ||
+ | |||
+ | </div> | ||
<div class="backgroundNotebook"> | <div class="backgroundNotebook"> | ||
− | <div class=" | + | <div class="photoMaterials"><h1 class="bigtitle">MATERIALS<h1></div> |
<div class="content"> | <div class="content"> | ||
− | + | <br><br> | |
− | <p class=" | + | <p class="first" id="ca1">Antibiotics</p> |
− | <table border="1.5"> | + | <table border="1.5" class="table"> |
<tr style="background:#FFEE99"> | <tr style="background:#FFEE99"> | ||
<td><strong>Name</strong></td> | <td><strong>Name</strong></td> | ||
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<td>Ampicillin</td> | <td>Ampicillin</td> | ||
<td>SIGMA</td> | <td>SIGMA</td> | ||
+ | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
Line 170: | Line 185: | ||
</tr> | </tr> | ||
</table> | </table> | ||
− | <p class=" | + | <p class="first" id="ca2">Antibody</p> |
− | <table border="1.5"> | + | <table border="1.5" class="table"> |
<tr style="background:#FFEE99"> | <tr style="background:#FFEE99"> | ||
<td><strong>Name</strong></td> | <td><strong>Name</strong></td> | ||
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<td>Anti-V5 mouse Monoclonal</td> | <td>Anti-V5 mouse Monoclonal</td> | ||
<td>Yao-Hong Biotechnology</td> | <td>Yao-Hong Biotechnology</td> | ||
+ | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>Anti- | + | <td>Anti-FLAG mouse Monoclonal</td> |
<td>Yao-Hong Biotechnology</td> | <td>Yao-Hong Biotechnology</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Goat anti-Mouse IgG (H+L)-HRP </td> | ||
+ | <td> Leadgene Biomedical, Inc</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
− | <p class=" | + | <p class="first" id="ca3">Enzyme</p> |
− | <table border="1.5"> | + | <table border="1.5" class="table"> |
<tr style="background:#FFEE99"> | <tr style="background:#FFEE99"> | ||
<td><strong>Name</strong></td> | <td><strong>Name</strong></td> | ||
Line 224: | Line 244: | ||
</tr> | </tr> | ||
</table> | </table> | ||
− | <p class=" | + | <p class="first" id="ca4">Equipment</p> |
− | <table border="1.5"> | + | <table border="1.5" class="table"> |
<tr style="background:#FFEE99"> | <tr style="background:#FFEE99"> | ||
<td><strong>Equipment</strong></td> | <td><strong>Equipment</strong></td> | ||
Line 278: | Line 298: | ||
<tr> | <tr> | ||
<td>Hot plate</td> | <td>Hot plate</td> | ||
− | <td> | + | <td>Corning<sup>®</sup> hotplate stirrers</td> |
<td>SIBATA</td> | <td>SIBATA</td> | ||
</tr> | </tr> | ||
Line 297: | Line 317: | ||
</tr> | </tr> | ||
</table> | </table> | ||
− | <p class=" | + | <p class="first" id="ca5">Kits</p> |
− | <table border="1.5"> | + | <table border="1.5" class="table"> |
<tr style="background:#FFEE99"> | <tr style="background:#FFEE99"> | ||
<td><strong>Name</strong></td> | <td><strong>Name</strong></td> | ||
Line 305: | Line 325: | ||
<tr> | <tr> | ||
<td>Pichia Expression Kit, original kit</td> | <td>Pichia Expression Kit, original kit</td> | ||
− | <td> | + | <td> Invitrogen™</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Line 316: | Line 336: | ||
</tr> | </tr> | ||
</table> | </table> | ||
− | <p class=" | + | <p class="first" id="ca6">Markers</p> |
− | <table border="1.5"> | + | <table border="1.5" class="table"> |
<tr style="background:#FFEE99"> | <tr style="background:#FFEE99"> | ||
<td><strong>Name</strong></td> | <td><strong>Name</strong></td> | ||
Line 327: | Line 347: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
+ | <td>Omics RGB Prestained Protein Ladder </td> | ||
+ | <td>Omics Bio</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | |||
<td>DNA Gel Loading Dye (6X)</td> | <td>DNA Gel Loading Dye (6X)</td> | ||
<td>YEASTERN BIOTECH</td> | <td>YEASTERN BIOTECH</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
− | <p class=" | + | <p class="first" id="ca7">Media</p> |
− | <table border="1.5"> | + | <table border="1.5" class="table"> |
<tr style="background:#FFEE99"> | <tr style="background:#FFEE99"> | ||
<td><strong>Media</strong></td> | <td><strong>Media</strong></td> | ||
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<tr> | <tr> | ||
<td>LB medium</td> | <td>LB medium</td> | ||
− | <td>10 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L | + | <td>10 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH<sub>2</sub>O</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>LB medium(low salt)</td> | <td>LB medium(low salt)</td> | ||
− | <td>5 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L | + | <td>5 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH<sub>2</sub>O</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Yeast Extract–Peptone–Dextrose (YPD) Medium</td> | <td>Yeast Extract–Peptone–Dextrose (YPD) Medium</td> | ||
− | <td>10 g yeast extract , 20 g peptone ,20g Dextrose in 1 L | + | <td>10 g yeast extract , 20 g peptone ,20g Dextrose in 1 L ddH<sub>2</sub>O</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Standard Minimal Medium (SD)</td> | <td>Standard Minimal Medium (SD)</td> | ||
<td>Yeast Nitrogen Base amino acids 6.7g<br> | <td>Yeast Nitrogen Base amino acids 6.7g<br> | ||
− | + | H<sub>2</sub>O 850ml<br> | |
50% Glucose 40ml<br> | 50% Glucose 40ml<br> | ||
− | + | 10×-His DO solution 100ml | |
</td> | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
+ | <br><br><br> | ||
</div> | </div> | ||
</div> | </div> |
Latest revision as of 08:53, 1 December 2018
MATERIALS
Antibiotics
Name | Supplier |
Zeocin™ (solution) | Thermo Fisher |
Ampicillin | SIGMA |
Chloramphenicol | SIGMA |
Antibody
Name | Supplier |
Anti-HA mouse Monoclonal | Yao-Hong Biotechnology |
Anti-V5 mouse Monoclonal | Yao-Hong Biotechnology |
Anti-FLAG mouse Monoclonal | Yao-Hong Biotechnology |
Goat anti-Mouse IgG (H+L)-HRP | Leadgene Biomedical, Inc |
Enzyme
Name | Supplier |
BamHI | New England BioLabs |
AseI | New England BioLabs |
EcoRI | New England BioLabs |
SalI | New England BioLabs |
AgeI | New England BioLabs |
PstI | New England BioLabs |
AvrII | New England BioLabs |
Equipment
Equipment | Name | Manufacturer |
Table Centrifuge | Centrifuge 5415R | eppendorf |
PCR-Machine | CYCLER-25 | major science |
PCR-Machine | SimpliAmp™ Thermal Cycler | Life technology |
UV light box | UV light box | TOPBIO |
Digital gel image system | DigiGel DGIS-11c | TOPBIO |
Water bath | Water bath B206 | FIRSTEK |
Heat block | Dry bath incubator | major science |
Scale | PB3002-S/PH DeltaRange | Mettler Toledo |
NanoDrop | ND-1000 UV-Vis Spectrophotometer | Marshall Scientific |
Hot plate | Corning® hotplate stirrers | SIBATA |
Micro-computer compact ultra pure water system | Micro-computer compact ultra pure water system PPI-185UV | Uniss |
Vortex mixer | Vortex-Genie 2 SI-0236 | Scientific Industries |
Multiskan | Multiskan™ FC Microplate Photometer | Thermo |
Kits
Name | Supplier |
Pichia Expression Kit, original kit | Invitrogen™ |
Miniprep | Biokit |
Gel extraction | Biokit |
Markers
Name | Supplier |
1 kb DNA marker XR-LYD008-1ML | YEASTERN BIOTECH |
Omics RGB Prestained Protein Ladder | Omics Bio |
DNA Gel Loading Dye (6X) | YEASTERN BIOTECH |
Media
Media | Composition |
LB medium | 10 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH2O |
LB medium(low salt) | 5 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH2O |
Yeast Extract–Peptone–Dextrose (YPD) Medium | 10 g yeast extract , 20 g peptone ,20g Dextrose in 1 L ddH2O |
Standard Minimal Medium (SD) | Yeast Nitrogen Base amino acids 6.7g H2O 850ml 50% Glucose 40ml 10×-His DO solution 100ml |