Difference between revisions of "Team:CCU Taiwan/Materials"

 
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'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div>
 
'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div>
  
−
                     <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a><hr>
+
                     <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a>
 
                         <ul class="sub" id="sub_home" style="cursor:default;">
 
                         <ul class="sub" id="sub_home" style="cursor:default;">
 
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a>
 
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a>
−
<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home2">Medals</li></a>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a>
−
<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home3">For Judges</li></a>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements"><li class="list" id="home5">Achievements</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
−
                     <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a><hr>
+
                     <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a>
 
<ul class="sub" id="sub_project" style="cursor:default;">
 
<ul class="sub" id="sub_project" style="cursor:default;">
 
    <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a>
 
    <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a>
−
<a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab "><li class="list" id="project5">InterLab</li></a>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab"><li class="list" id="project5">InterLab</li></a>
 
</ul>
 
</ul>
 
</li>
 
</li>
−
                     <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a><hr>
+
                     <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a>
 
                         <ul class="sub" id="sub_parts" style="cursor:default;">
 
                         <ul class="sub" id="sub_parts" style="cursor:default;">
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Parts"><li class="list" id="parts1">Overview</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a>
−
<a href="https://2018.igem.org/Team:CCU_Taiwan/Improve"><li class="list" id="parts3">Improved Part</li></a>
 
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
−
                     <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a><hr>
+
                     <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a>
 
                         <ul class="sub" id="sub_modeling" style="cursor:default;">
 
                         <ul class="sub" id="sub_modeling" style="cursor:default;">
 
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a>
 
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a>
−
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding</li></a>
+
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding Model</li></a>
−
<a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer</li></a>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer Model</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
−
                     <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a><hr>
+
                     <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a>
 
                         <ul class="sub" id="sub_drylab" style="cursor:default;">
 
                         <ul class="sub" id="sub_drylab" style="cursor:default;">
 
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a>
 
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a>
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                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
−
    <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a><hr>
+
    <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a>
 
                         <ul class="sub" id="sub_human_practice" style="cursor:default;">
 
                         <ul class="sub" id="sub_human_practice" style="cursor:default;">
−
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Overview</li></a>
+
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Human Practice</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a>
−
<a href="https://2018.igem.org/Team:CCU_Taiwan/Intergrate"><li class="list" id="human_practice5">Intergrated HP</li></a>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Integrate"><li class="list" id="human_practice5">Integrated HP</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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     </header>
 
     </header>
 +
 +
 +
<div class="indicator">
 +
 +
<div class="pointerNotebook" id="1"><a href="#ca1">Antibiotics</a></div>
 +
<div class="pointerNotebook" id="2"><a href="#ca2">Antibody</a></div>
 +
<div class="pointerNotebook" id="3"><a href="#ca3">Enzyme</a></div>
 +
<div class="pointerNotebook" id="4"><a href="#ca4">Equipment</a></div>
 +
<div class="pointerNotebook" id="5"><a href="#ca5">Kits</a></div>
 +
<div class="pointerNotebook" id="4"><a href="#ca6">Markers</a></div>
 +
<div class="pointerNotebook" id="5"><a href="#ca7">Media</a></div>
 +
 +
</div>
 
<div class="backgroundNotebook">
 
<div class="backgroundNotebook">
−
<div class="bcg"></div>
+
<div class="photoMaterials"><h1 class="bigtitle">MATERIALS<h1></div>
 
       <div class="content">
 
       <div class="content">
−
<p class="first">Materials</p>
+
<br><br>
−
<p class="second">Antibiotics</p>
+
<p class="first" id="ca1">Antibiotics</p>
 
<table border="1.5" class="table">
 
<table border="1.5" class="table">
 
<tr style="background:#FFEE99">
 
<tr style="background:#FFEE99">
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<td>Ampicillin</td>
 
<td>Ampicillin</td>
 
<td>SIGMA</td>
 
<td>SIGMA</td>
 +
                       
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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</tr>
 
</tr>
 
</table>
 
</table>
−
<p class="second">Antibody</p>
+
<p class="first" id="ca2">Antibody</p>
 
<table border="1.5" class="table">
 
<table border="1.5" class="table">
 
<tr style="background:#FFEE99">
 
<tr style="background:#FFEE99">
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<td>Anti-V5 mouse Monoclonal</td>
 
<td>Anti-V5 mouse Monoclonal</td>
 
<td>Yao-Hong Biotechnology</td>
 
<td>Yao-Hong Biotechnology</td>
 +
 
</tr>
 
</tr>
 
<tr>
 
<tr>
−
<td>Anti-Flag mouse Monoclonal</td>
+
<td>Anti-FLAG mouse Monoclonal</td>
 
<td>Yao-Hong Biotechnology</td>
 
<td>Yao-Hong Biotechnology</td>
 +
</tr>
 +
<tr>
 +
<td>Goat anti-Mouse IgG (H+L)-HRP </td>
 +
<td> Leadgene Biomedical, Inc</td>
 
</tr>
 
</tr>
 
</table>
 
</table>
−
<p class="second">Enzyme</p>
+
<p class="first" id="ca3">Enzyme</p>
 
<table border="1.5" class="table">
 
<table border="1.5" class="table">
 
<tr style="background:#FFEE99">
 
<tr style="background:#FFEE99">
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</tr>
 
</tr>
 
</table>
 
</table>
−
<p class="second">Equipment</p>
+
<p class="first" id="ca4">Equipment</p>
 
<table border="1.5" class="table">
 
<table border="1.5" class="table">
 
<tr style="background:#FFEE99">
 
<tr style="background:#FFEE99">
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<tr>
 
<tr>
 
<td>Hot plate</td>
 
<td>Hot plate</td>
−
<td>Corning® hotplate stirrers</td>
+
<td>Corning<sup>®</sup> hotplate stirrers</td>
 
<td>SIBATA</td>
 
<td>SIBATA</td>
 
</tr>
 
</tr>
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</tr>
 
</tr>
 
</table>
 
</table>
−
<p class="second">Kits</p>
+
<p class="first" id="ca5">Kits</p>
 
<table border="1.5" class="table">
 
<table border="1.5" class="table">
 
<tr style="background:#FFEE99">
 
<tr style="background:#FFEE99">
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<tr>
 
<tr>
 
<td>Pichia Expression Kit, original kit</td>
 
<td>Pichia Expression Kit, original kit</td>
−
<td>Biokit</td>
+
<td> Invitrogen™</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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</tr>
 
</tr>
 
</table>
 
</table>
−
<p class="second">Markers</p>
+
<p class="first" id="ca6">Markers</p>
 
<table border="1.5" class="table">
 
<table border="1.5" class="table">
 
<tr style="background:#FFEE99">
 
<tr style="background:#FFEE99">
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</tr>
 
</tr>
 
<tr>
 
<tr>
 +
                                <td>Omics RGB Prestained Protein Ladder </td>
 +
<td>Omics Bio</td>
 +
</tr>
 +
<tr>
 +
 
<td>DNA Gel Loading Dye (6X)</td>
 
<td>DNA Gel Loading Dye (6X)</td>
 
<td>YEASTERN BIOTECH</td>
 
<td>YEASTERN BIOTECH</td>
 
</tr>
 
</tr>
 
</table>
 
</table>
−
<p class="second">Media</p>
+
<p class="first" id="ca7">Media</p>
 
<table border="1.5" class="table">
 
<table border="1.5" class="table">
 
<tr style="background:#FFEE99">
 
<tr style="background:#FFEE99">
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<tr>
 
<tr>
 
<td>LB medium</td>
 
<td>LB medium</td>
−
<td>10 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH2O</td>
+
<td>10 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH<sub>2</sub>O</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td>LB medium(low salt)</td>
 
<td>LB medium(low salt)</td>
−
<td>5 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH2O</td>
+
<td>5 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH<sub>2</sub>O</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td>Yeast Extract–Peptone–Dextrose (YPD) Medium</td>
 
<td>Yeast Extract–Peptone–Dextrose (YPD) Medium</td>
−
<td>10 g yeast extract , 20 g peptone ,20g Dextrose in 1 L ddH2O</td>
+
<td>10 g yeast extract , 20 g peptone ,20g Dextrose in 1 L ddH<sub>2</sub>O</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td>Standard Minimal Medium (SD)</td>
 
<td>Standard Minimal Medium (SD)</td>
 
<td>Yeast Nitrogen Base amino acids 6.7g<br>
 
<td>Yeast Nitrogen Base amino acids 6.7g<br>
−
H2O 865ml<br>
+
H<sub>2</sub>O 850ml<br>
 
50% Glucose 40ml<br>
 
50% Glucose 40ml<br>
−
Nutrient (without Histidine) 100ml
+
10×-His DO solution 100ml
 
</td>
 
</td>
 
</tr>
 
</tr>
 
</table>
 
</table>
 +
<br><br><br>
 
       </div>
 
       </div>
 
</div>
 
</div>

Latest revision as of 08:53, 1 December 2018

MATERIALS



Antibiotics

Name Supplier
Zeocin™ (solution) Thermo Fisher
Ampicillin SIGMA
Chloramphenicol SIGMA

Antibody

Name Supplier
Anti-HA mouse Monoclonal Yao-Hong Biotechnology
Anti-V5 mouse Monoclonal Yao-Hong Biotechnology
Anti-FLAG mouse Monoclonal Yao-Hong Biotechnology
Goat anti-Mouse IgG (H+L)-HRP Leadgene Biomedical, Inc

Enzyme

Name Supplier
BamHI New England BioLabs
AseI New England BioLabs
EcoRI New England BioLabs
SalI New England BioLabs
AgeI New England BioLabs
PstI New England BioLabs
AvrII New England BioLabs

Equipment

Equipment Name Manufacturer
Table Centrifuge Centrifuge 5415R eppendorf
PCR-Machine CYCLER-25 major science
PCR-Machine SimpliAmp™ Thermal Cycler Life technology
UV light box UV light box TOPBIO
Digital gel image system DigiGel DGIS-11c TOPBIO
Water bath Water bath B206 FIRSTEK
Heat block Dry bath incubator major science
Scale PB3002-S/PH DeltaRange Mettler Toledo
NanoDrop ND-1000 UV-Vis Spectrophotometer Marshall Scientific
Hot plate Corning® hotplate stirrers SIBATA
Micro-computer compact ultra pure water system Micro-computer compact ultra pure water system PPI-185UV Uniss
Vortex mixer Vortex-Genie 2 SI-0236 Scientific Industries
Multiskan Multiskan™ FC Microplate Photometer Thermo

Kits

Name Supplier
Pichia Expression Kit, original kit Invitrogen™
Miniprep Biokit
Gel extraction Biokit

Markers

Name Supplier
1 kb DNA marker XR-LYD008-1ML YEASTERN BIOTECH
Omics RGB Prestained Protein Ladder Omics Bio
DNA Gel Loading Dye (6X) YEASTERN BIOTECH

Media

Media Composition
LB medium 10 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH2O
LB medium(low salt) 5 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH2O
Yeast Extract–Peptone–Dextrose (YPD) Medium 10 g yeast extract , 20 g peptone ,20g Dextrose in 1 L ddH2O
Standard Minimal Medium (SD) Yeast Nitrogen Base amino acids 6.7g
H2O 850ml
50% Glucose 40ml
10×-His DO solution 100ml