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<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a> | ||
+ | <a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements"><li class="list" id="home5">Achievements</li></a> | ||
</ul> | </ul> | ||
</li> | </li> | ||
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a> | ||
− | <a href="https://2018.igem.org/Team:CCU_Taiwan/ | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Integrate"><li class="list" id="human_practice5">Integrated HP</li></a> |
</ul> | </ul> | ||
</li> | </li> | ||
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<td>Ampicillin</td> | <td>Ampicillin</td> | ||
<td>SIGMA</td> | <td>SIGMA</td> | ||
+ | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>Anti-V5 mouse Monoclonal</td> | <td>Anti-V5 mouse Monoclonal</td> | ||
<td>Yao-Hong Biotechnology</td> | <td>Yao-Hong Biotechnology</td> | ||
+ | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Anti-FLAG mouse Monoclonal</td> | <td>Anti-FLAG mouse Monoclonal</td> | ||
<td>Yao-Hong Biotechnology</td> | <td>Yao-Hong Biotechnology</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Goat anti-Mouse IgG (H+L)-HRP </td> | ||
+ | <td> Leadgene Biomedical, Inc</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td> | + | <td>Omics RGB Prestained Protein Ladder </td> |
− | <td> | + | <td>Omics Bio</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>Standard Minimal Medium (SD)</td> | <td>Standard Minimal Medium (SD)</td> | ||
<td>Yeast Nitrogen Base amino acids 6.7g<br> | <td>Yeast Nitrogen Base amino acids 6.7g<br> | ||
− | H<sub>2</sub>O | + | H<sub>2</sub>O 850ml<br> |
50% Glucose 40ml<br> | 50% Glucose 40ml<br> | ||
− | + | 10×-His DO solution 100ml | |
</td> | </td> | ||
</tr> | </tr> |
Latest revision as of 08:53, 1 December 2018
MATERIALS
Antibiotics
Name | Supplier |
Zeocin™ (solution) | Thermo Fisher |
Ampicillin | SIGMA |
Chloramphenicol | SIGMA |
Antibody
Name | Supplier |
Anti-HA mouse Monoclonal | Yao-Hong Biotechnology |
Anti-V5 mouse Monoclonal | Yao-Hong Biotechnology |
Anti-FLAG mouse Monoclonal | Yao-Hong Biotechnology |
Goat anti-Mouse IgG (H+L)-HRP | Leadgene Biomedical, Inc |
Enzyme
Name | Supplier |
BamHI | New England BioLabs |
AseI | New England BioLabs |
EcoRI | New England BioLabs |
SalI | New England BioLabs |
AgeI | New England BioLabs |
PstI | New England BioLabs |
AvrII | New England BioLabs |
Equipment
Equipment | Name | Manufacturer |
Table Centrifuge | Centrifuge 5415R | eppendorf |
PCR-Machine | CYCLER-25 | major science |
PCR-Machine | SimpliAmp™ Thermal Cycler | Life technology |
UV light box | UV light box | TOPBIO |
Digital gel image system | DigiGel DGIS-11c | TOPBIO |
Water bath | Water bath B206 | FIRSTEK |
Heat block | Dry bath incubator | major science |
Scale | PB3002-S/PH DeltaRange | Mettler Toledo |
NanoDrop | ND-1000 UV-Vis Spectrophotometer | Marshall Scientific |
Hot plate | Corning® hotplate stirrers | SIBATA |
Micro-computer compact ultra pure water system | Micro-computer compact ultra pure water system PPI-185UV | Uniss |
Vortex mixer | Vortex-Genie 2 SI-0236 | Scientific Industries |
Multiskan | Multiskan™ FC Microplate Photometer | Thermo |
Kits
Name | Supplier |
Pichia Expression Kit, original kit | Invitrogen™ |
Miniprep | Biokit |
Gel extraction | Biokit |
Markers
Name | Supplier |
1 kb DNA marker XR-LYD008-1ML | YEASTERN BIOTECH |
Omics RGB Prestained Protein Ladder | Omics Bio |
DNA Gel Loading Dye (6X) | YEASTERN BIOTECH |
Media
Media | Composition |
LB medium | 10 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH2O |
LB medium(low salt) | 5 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH2O |
Yeast Extract–Peptone–Dextrose (YPD) Medium | 10 g yeast extract , 20 g peptone ,20g Dextrose in 1 L ddH2O |
Standard Minimal Medium (SD) | Yeast Nitrogen Base amino acids 6.7g H2O 850ml 50% Glucose 40ml 10×-His DO solution 100ml |