Difference between revisions of "Team:Tianjin"

 
(82 intermediate revisions by 6 users not shown)
Line 1: Line 1:
 
{{Tianjin}}
 
{{Tianjin}}
 +
<!DOCTYPE html>
 
<html>
 
<html>
 +
<head>
 +
<!--loading-->
 +
<style type="text/css">
 +
    body{margin: 0px;}
 +
    .loading{
 +
        width:100%;height:100%;z-index:99999999;position:fixed;text-align:center;margin-top:-24px;
 +
        background-color: #79acdd;background: url(https://static.igem.org/mediawiki/2018/0/0e/T--Tianjin--loading.gif);
 +
    }
 +
    </style>
  
−
<img src=https://static.igem.org/mediawiki/2018/5/5d/T--Tianjin--doge.jpg>
+
    <div class="loading">
−
My files have not been uploaded.
+
    </div>
 +
    <script type="text/javascript">
 +
        $(document).ready(function(){
 +
          setTimeout(function(){
 +
            $(".loading").remove();
 +
        },0);
  
−
<div class="column full_size" >
+
    })
−
<h1> Welcome to iGEM 2018! </h1>
+
    </script>
−
<p>Your team has been approved and you are ready to start the iGEM season! </p>
+
  
 +
<script src="https://2018.igem.org/Team:Tianjin/js/jquery?action=raw&ctype=text/javascript"></script>
 +
        <meta charset="utf-8">
 +
        <meta http-equiv="X-UA-Compatible" content="IE=Edge,chrome=1"/>
 +
        <meta content="width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=0;" name="viewport">
 +
        <meta name="renderer" content="webkit">
 +
        <meta name="author" content="773715181 Jiaxiao Han">
 +
        <meta content="2018iGEM Team:Tianjin Kai System to control biological clock" name="description">
 +
        <meta content="Team:Tianjin,iGEM:Tianjin,iGEM,2018iGEM" name="keywords">
 +
        <meta content="biological clock,clock,Kai,Kai A,Kai B,KaiC" name="keywords">
 +
<title>Team:Tianjin - 2018.igem.org</title>
 +
        <link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:Tianjin/css/bootstrap?action=raw&ctype=text/css">
 +
        <link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:Tianjin/css/base?action=raw&ctype=text/css">
 +
        <link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:Tianjin/css/font-awesome?action=raw&ctype=text/css">
 +
        <link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:Tianjin/css/text?action=raw&ctype=text/css">
 +
        <script src="https://2018.igem.org/Team:Tianjin/js/bootstrap?action=raw&ctype=text/javascript"></script>
 +
        <link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:Tianjin/css/timeline?action=raw&ctype=text/css">
 +
<!-- delete.css  -->
 +
<style>
 +
#globalWrapper,#content{background-color:transparent;border:0;border-bottom:0;float:none;margin:0;padding:0;width:100%;list-style-image:none}#top_title{margin:0}#HQ_page th{background-color:transparent;padding:0;color:black;border:0;border-collapse:collapse;vertical-align:text-top;text-transform:uppercase;text-align:center}#HQ_page td{padding:0;border:0;vertical-align:text-top;text-align:center;line-height:35px}#HQ_page .jumbotron p{text-align:center;margin-bottom:15px;font-weight:200}#HQ_page p{text-align:center}body{margin-bottom:-10px}#top_title,#sideMenu{display:none}a:hover{text-decoration:none}.mw-content-ltr ul{padding-top:0;padding-right:0;margin:auto}body{margin-top:0}#wrapper{padding-left:0}#page-wrapper{width:100%;padding:0}.intro{background-size:cover;width:100%;position:relative}.huge{font-size:50px;line-height:normal}
 +
</style>
  
−
<img src="http://placehold.it/1080x320/c4baba/e4dede">
+
<style>
−
+
#top_title{display:none;}
 +
.igem_2018_team_menu{display:none !important;}
 +
</style>
  
−
</div>  
+
<script>
 +
$('#top_title').remove();$("#globalWrapper").removeAttr("id");$("#content").removeAttr("id");$("#HQ_page").removeAttr("id");$('.mw-body').removeClass('mw-body');$('.igem_2018_team_mobile_bar').remove();
 +
$('#bodyContent').removeAttr("id");
 +
$('#mw-content-text').removeAttr("id");
 +
$('.mw-content-ltr').removeAttr("class");
 +
$('.igem_2018_team_menu displaying_menu').remove();
 +
$('.igem_2018_team_content').removeAttr("class");
 +
$('.igem_2018_team_column_wrapper').removeAttr("class");
 +
$('.igem_2018_team_menu').remove();
 +
</script>
 +
</head>
 +
<body>
 +
    <nav class="navbar navbar-default navbar-fixed-top" role="navigation" style="background-color:#79acdd;margin-top:14px;">
 +
    <div class="container-fluid">
 +
        <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target=".navbar-main-collapse">
 +
            <i class="fa fa-bars"></i>
 +
        </button>
 +
    <div class="navbar-header">
 +
        <div class="navbar-brand" href="https://2018.igem.org/Team:Tianjin">
 +
            <a href="https://2018.igem.org/Team:Tianjin">
 +
            <div class="circle" style="background-image:url(https://static.igem.org/mediawiki/2018/4/45/T--Tianjin--logo.png);left: 50px;">
 +
            </div>
 +
            </a>
 +
        </div>
 +
    </div>
 +
        <div class="navbar-collapse navbar-right navbar-main-collapse collapse">
 +
        <ul class="nav navbar-nav navbar-right" style="margin: 15px;">
 +
            <li class="dropdown">
 +
                <a href="#" class="dropdown-toggle" data-toggle="dropdown">
 +
                    PROJECT
 +
                    <b class="caret"></b>
 +
                </a>
 +
                <ul class="dropdown-menu" style="left: -14px;">
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Description">BACKGROUND</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Design">DESIGN</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Experiments">EXPERIMENTS</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Notebook">NOTEBOOK</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Model">MODEL</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Measurement">MEASUREMENT</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Demonstrate">DEMONSTRATE</a></li>
 +
                </ul>
 +
            </li>
 +
            <li class="dropdown">
 +
                <a href="#" class="dropdown-toggle" data-toggle="dropdown">
 +
                    PARTS
 +
                    <b class="caret"></b>
 +
                </a>
 +
                <ul class="dropdown-menu" style="left: -26px;">
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Parts">PARTS OVERVIEW</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Basic_Part">BASIC PARTS</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Composite_Part">COMPOSITE PARTS</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Part_Collection">PART COLLECTION</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Improve">IMPROVED PART</a></li>
 +
                </ul>
 +
            </li>
 +
            <li>
 +
                <a href="https://2018.igem.org/Team:Tianjin/Safety">
 +
                    SAFETY             
 +
                </a>
 +
            </li>
 +
            <li class="dropdown">
 +
                <a href="#" class="dropdown-toggle" data-toggle="dropdown">
 +
                    HUMAN PRACTICES
 +
                    <b class="caret"></b>
 +
                </a>
 +
                <ul class="dropdown-menu">
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Human_Practices">INTEGRATED</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Collaborations">COLLABORATION</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Public_Engagement">PUBLIC ENGAGEMENT</a></li>
 +
                </ul>
 +
            </li>
 +
            <li class="dropdown">
 +
                <a href="#" class="dropdown-toggle" data-toggle="dropdown">
 +
                    TEAM
 +
                    <b class="caret"></b>
 +
                </a>
 +
                <ul class="dropdown-menu" style="left: -29px;">
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Team">MEMBERS</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:Tianjin/Attributions">ATTRIBUTIONS</a></li>
 +
                </ul>
 +
            </li>
 +
            <li>
 +
                <a href="https://2018.igem.org/Team:Tianjin/Judging">
 +
                    FOR JUDGES             
 +
                </a>
 +
            </li>
 +
        </ul>
 +
    </div>
 +
    </div>
 +
    </nav>
 +
    <script type="text/javascript" src="https://2018.igem.org/Team:Tianjin/js/navigation?action=raw&ctype=text/javascript"></script>
  
 +
    <div id="fill" style="width: 100%;margin-top:-16px;"></div>
  
−
<div class="column full_size" >
+
    <div id="MainPic">
−
 
+
    <div id="bannercontent">
−
<h3>Before you start</h3>
+
            <div id="BgChange">
−
<p> Please read the following pages:</p>
+
                <img src="https://static.igem.org/mediawiki/2018/3/35/T--Tianjin--Day.jpg" alt="https://static.igem.org/mediawiki/2018/8/8f/T--Tianjin--Night.jpg" style="width: 100%;">
−
<ul>
+
            </div>
−
<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
+
            <div class="bulbBox">
−
<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
+
                <div id="change" class="bulb">
−
<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
+
                   
−
</ul>
+
                </div>
−
</div>
+
            </div>
−
 
+
        </div>
−
 
+
    </div>
−
<div class="clear extra_space"></div>
+
    <div class="timeline">
−
<div class="line_divider"></div>
+
        <div class="toolate">
−
<div class="clear extra_space"></div>
+
            <div class="timeline-text fo">
−
 
+
                <p>
−
 
+
                    Circadian rhythm control over biological processes is pervasive among multicellular eukaryotes and cyanobacteria, suggesting a common evolved network motif and a strong evolutionary advantage for anticipating regular fluctuations in the environment.<br> To uncover the mysteries of the biological evolution of the circadian rhythm system, our project combines non-circadian single-cell eukaryotic <em>Saccharomyces cerevisiae</em> with the circadian clock of autotrophic circadian cyanobacteria. According to the concept of “understanding by creating (creating is understanding)” in synthetic biology, a new biological clock system has been established in <em>Saccharomyces cerevisiae</em>, which has inspired a series of follow-up studies and novel applications of our project.
−
 
+
                </p>
−
<div class="column full_size" >
+
            </div>
−
<h3> Styling your wiki </h3>
+
        </div>
−
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+
    <span class="timeline-bar timeline-center">
−
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
+
    <span class="timeline-bar-fill">
−
 
+
   
−
</div>
+
    </span>
−
 
+
    </span>
−
 
+
    <div class="timeline-states">
−
 
+
    <div class="timeline-state">
−
 
+
    <div class="timeline-content">
−
<div class="clear extra_space"></div>
+
    <div class="timeline-title right">Project</div>
−
 
+
    <div class="timeline-text">
−
 
+
                    <p>
−
 
+
                        We successfully reconstructed the KaiABC circadian clock in Saccharomyces cerevisiae, predicting by modeling, regulating by expression control and characterizing its oscillation by yeast two-hybrid system. A bold attempt is made to explore how the oscillator can impact chromatin structure in yeast. These results demonstrate that the heterologous circadian oscillator is transplantable for metabolic pathway study as well as wide-ranging applications.
−
<div class="column third_size" >
+
                    </p>
−
 
+
                    </div>
−
<h3> Uploading pictures and files </h3>
+
    </div>
−
<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
+
    <div class="timeline-content">
−
 
+
                    <div class="timeline-title left">Safety</div>
−
 
+
                    <div class="timeline-text">
−
<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
+
                    <p>
−
 
+
                      Although the project barely involves genetic pollution or the use of toxic reagents, our instructor still attaches great importance to the lab safety during the experiment. Before entering the lab, every operator must accept comprehensive training and have a deep understanding of equipment and reagents. All wastes are divided and gathered into special bottles, later get handled by professionals in a unified manner.
−
<div class="button_link">
+
                    </p>
−
<a href="https://2018.igem.org/Special:Upload">
+
                    </div>
−
UPLOAD FILES
+
                </div>
−
</a>
+
    <div class="timeline-icon timeline-center"></div>
−
</div>
+
                <div class="timeline-icon timeline-center"></div>
−
 
+
    </div>
−
</div>
+
    <div class="timeline-state">
−
 
+
    <div class="timeline-content">
−
<div class="column third_size" >
+
    <div class="timeline-title right">Human Practice</div>
−
<h3> Wiki template information </h3>
+
    <div class="timeline-text">
−
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
+
                    <p>
−
 
+
                      We made a survey about the circadian clock to investigate the potential impact to society and conducted follow-up market research on possible application. Furthermore, we were devoted to spreading iGEM idea to every age group. We made a picture book and performed a TV show for children, invited teenagers and parents to our lab and displayed biology to hundreds of tourists on the Begonia Festival. Synthetic biology can be a popular science by our actual action.
−
</div>  
+
                    </p>
−
 
+
                    </div>
−
 
+
    </div>
−
 
+
    <div class="timeline-content">
−
<div class="column third_size" >
+
                    <div class="timeline-title left">Team</div>
−
<div class="highlight decoration_B_full">
+
                    <div class="timeline-text">
−
<h3> Editing your wiki </h3>
+
                    <p>
−
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
+
                        Twelve years have passed since Professor Yingjin Yuan worked with MIT and successfully promoted the iGEM in Asia. iGEM has been all the rage in Tianjin University. This year, a group of dynastic teenagers have gone through a series of tough tests and eventually set up a united family. The wonderful summer will be kept in everyone’s mind. Thanks for everyone who once reached out to us.
−
<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
+
                    </p>
−
 
+
                    </div>
−
<div class="button_link">
+
                </div>
−
<a href="https://2018.igem.org/wiki/index.php?title=Team:Tianjin&action=edit">  
+
    <div class="timeline-icon timeline-center"></div>
−
EDIT PAGE
+
                <div class="timeline-icon timeline-center"></div>
−
</a>
+
    </div>
−
</div>
+
    <div class="timeline-state">
−
 
+
    <div class="timeline-content">
−
 
+
    <div class="timeline-title right">Parts</div>
−
</div>
+
    <div class="timeline-text">
−
</div>
+
                    <p>
−
 
+
                        We attach great importance to the construction and appliance of BioBrick Part. We submitted a variety of parts centering on core KaiABC oscillator, from single genes to cassettes with fusion proteins in yeast two-hybrid system. Effective cassettes about reporter genes and downstream application were also constructed and submitted. Besides, we redesign previous Nanoluc to serve our project better.
−
 
+
                    </p>
−
 
+
                    </div>
−
 
+
    </div>
−
 
+
    <div class="timeline-content">
−
<div class="clear extra_space"></div>
+
                    <div class="timeline-title left">Model</div>
−
<div class="line_divider"></div>
+
                    <div class="timeline-text">
−
<div class="clear extra_space"></div>
+
                    <p>
−
 
+
                        The models include four parts. First, we established a fluorescent protein model using grayscale analysis to screen out the most suitable fluorescent proteins. Then, we drew the growth curve of yeasts and it fitted logistic model. It described the growth situation of the yeasts after plasmid introduction and it also offers the best measuring point and the best measuring interval. What’s more, we drew the degradation curve of the fluorescent protein, which helped us know different characteristics of the two chosen fluorescent proteins better. Finally, we constructed a model to illustrate the oscillation of KaiA, KaiB and KaiC protein called Mars Model, it also explained nicely the reason why the cycle reduced in yeasts.                    </p>
−
 
+
                    </div>
−
 
+
                </div>
−
<div class="column two_thirds_size" >
+
    <div class="timeline-icon timeline-center"></div>
−
<h3>Tips</h3>
+
                <div class="timeline-icon timeline-center"></div>
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
    </div>
−
<ul>
+
   
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
    </div>
−
<li>Be clear about what you are doing and how you plan to do this.</li>
+
    </div>
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
+
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
+
−
<li>Have lots of fun! </li>
+
−
</ul>  
+
−
</div>
+
−
 
+
−
 
+
−
<div class="column third_size">
+
−
<div class="highlight decoration_A_full">
+
−
<h3>Inspiration</h3>
+
−
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
+
−
<ul>
+
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
  
 +
    <script type="text/javascript" src="https://2018.igem.org/Team:Tianjin/js/timeline?action=raw&ctype=text/javascript"></script>
  
 +
    <!--<div style="text-align:center;color:#000;font-size:50px;margin-top: 200px;">
 +
        <p>Click</p>
 +
        <a style="color:darkorange" href="#" id="change">me</a>
 +
        <p>to change</p>
 +
        <p style="font-size:70px;color:papayawhip">TIME</p>
 +
    </div>
  
 +
    <div class="clock" id="clo">
 +
        <div class="h" style="transform: rotate(634.233deg);">
 +
            <div style=" width: 0px; height: 0px; position: relative; margin-left: auto; margin-right: auto; top: 33.3px; border-width: 0px 3px 66.7px 3px; border-style: solid; border-color: transparent transparent    white transparent; ">
 +
            </div>
 +
        </div>
 +
        <div class="m" style="transform: rotate(50.8deg);">
 +
            <div style=" width: 0px; height: 0px; position: relative; margin-left: auto; margin-right: auto; top: 0; border-width: 0px 2px 100px 2px; border-style: solid; border-color: transparent transparent white    transparent; ">
 +
            </div>
 +
        </div>
 +
    </div>-->
  
 +
    <div id="footer" style="background-color: #79acdd;text-align: center;margin-top: 0;">
 +
        <div class="container">
 +
            <div class="row">
 +
                <div class="col-sm-3 footer-part">
 +
                    <p>
 +
                        <i class="fa fa-weixin fa-lg"></i>
 +
                        IGEMTIANJIN
 +
                    </p>
 +
                </div>
 +
                <div class="col-sm-3 footer-part">
 +
                    <p>
 +
                        <i class="fa fa-twitter fa-lg"></i>
 +
                        IGEMTIANJIN2018
 +
                    </p>
 +
                </div>
 +
                <div class="col-sm-3 footer-part">
 +
                    <p>
 +
                        <i class="fa fa-envelope fa-lg"></i>
 +
                        HEBO@TJU.EDU.CN
 +
                    </p>
 +
                </div>
 +
                <div class="col-sm-3 footer-part">
 +
                    <p>
 +
                        <i class="fa fa-institution fa-lg"></i>
 +
                        BEIYANG CAMPUS,TIANJIN UNIVERSITY,TIANJIN
 +
                    </p>
 +
                </div>
 +
            </div>
 +
        </div>
 +
    </div>
 +
    <script type="text/javascript" src="https://2018.igem.org/Team:Tianjin/js/DayNight?action=raw&ctype=text/javascript"></script>
 +
    <!--<script type="text/javascript">
 +
    $(document).ready(function(){clock()});function clock(){setInterval(function(){var ms=new Date().getMilliseconds();var second=new Date().getSeconds();var minute=new Date().getMinutes()+second/60;var hour=new Date().getHours()+minute/60;var secondDegree=second*6;var minuteDegree=minute*6;var hourDegree=hour*30;var secondRotate="rotate("+secondDegree+"deg)";var minuteRotate="rotate("+minuteDegree+"deg)";var hourRotate="rotate("+hourDegree+"deg)";$("div.s").css({"transform":secondRotate,"-ms-transform":secondRotate,"-o-transform":secondRotate,"-moz-transform":secondRotate,"-webkit-transform":secondRotate});$("div.m").css({"transform":minuteRotate,"-ms-transform":minuteRotate,"-o-transform":minuteRotate,"-moz-transform":minuteRotate,"-webkit-transform":minuteRotate});$("div.h").css({"transform":hourRotate,"-ms-transform":hourRotate,"-o-transform":hourRotate,"-moz-transform":hourRotate,"-webkit-transform":hourRotate})},1000)};
 +
    </script>-->
 +
</body>
 
</html>
 
</html>

Latest revision as of 12:59, 6 December 2018

<!DOCTYPE html>

Team:Tianjin - 2018.igem.org
https://static.igem.org/mediawiki/2018/8/8f/T--Tianjin--Night.jpg

Circadian rhythm control over biological processes is pervasive among multicellular eukaryotes and cyanobacteria, suggesting a common evolved network motif and a strong evolutionary advantage for anticipating regular fluctuations in the environment.
To uncover the mysteries of the biological evolution of the circadian rhythm system, our project combines non-circadian single-cell eukaryotic Saccharomyces cerevisiae with the circadian clock of autotrophic circadian cyanobacteria. According to the concept of “understanding by creating (creating is understanding)” in synthetic biology, a new biological clock system has been established in Saccharomyces cerevisiae, which has inspired a series of follow-up studies and novel applications of our project.

Project

We successfully reconstructed the KaiABC circadian clock in Saccharomyces cerevisiae, predicting by modeling, regulating by expression control and characterizing its oscillation by yeast two-hybrid system. A bold attempt is made to explore how the oscillator can impact chromatin structure in yeast. These results demonstrate that the heterologous circadian oscillator is transplantable for metabolic pathway study as well as wide-ranging applications.

Safety

Although the project barely involves genetic pollution or the use of toxic reagents, our instructor still attaches great importance to the lab safety during the experiment. Before entering the lab, every operator must accept comprehensive training and have a deep understanding of equipment and reagents. All wastes are divided and gathered into special bottles, later get handled by professionals in a unified manner.

Human Practice

We made a survey about the circadian clock to investigate the potential impact to society and conducted follow-up market research on possible application. Furthermore, we were devoted to spreading iGEM idea to every age group. We made a picture book and performed a TV show for children, invited teenagers and parents to our lab and displayed biology to hundreds of tourists on the Begonia Festival. Synthetic biology can be a popular science by our actual action.

Team

Twelve years have passed since Professor Yingjin Yuan worked with MIT and successfully promoted the iGEM in Asia. iGEM has been all the rage in Tianjin University. This year, a group of dynastic teenagers have gone through a series of tough tests and eventually set up a united family. The wonderful summer will be kept in everyone’s mind. Thanks for everyone who once reached out to us.

Parts

We attach great importance to the construction and appliance of BioBrick Part. We submitted a variety of parts centering on core KaiABC oscillator, from single genes to cassettes with fusion proteins in yeast two-hybrid system. Effective cassettes about reporter genes and downstream application were also constructed and submitted. Besides, we redesign previous Nanoluc to serve our project better.

Model

The models include four parts. First, we established a fluorescent protein model using grayscale analysis to screen out the most suitable fluorescent proteins. Then, we drew the growth curve of yeasts and it fitted logistic model. It described the growth situation of the yeasts after plasmid introduction and it also offers the best measuring point and the best measuring interval. What’s more, we drew the degradation curve of the fluorescent protein, which helped us know different characteristics of the two chosen fluorescent proteins better. Finally, we constructed a model to illustrate the oscillation of KaiA, KaiB and KaiC protein called Mars Model, it also explained nicely the reason why the cycle reduced in yeasts.