Difference between revisions of "Team:TU-Eindhoven/Parts"

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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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            <div id="introDiv" class="col-xs-12 introduction">
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                <h1 style="text-align: center;">Parts overview</h1>
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            <div class="col-xs-12 col-sm-12 col-md-12 col-lg-12 col-xl-12 docs">
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<h3>Adding parts to the registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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                    <p>To realize our designed parts, we gratefully made use of the IDT Synthesis offer,
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                        allowing easy and fast access to the desired DNA constructs. All parts are codon
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                        optimized for expression in E. coli, the workhorse of our project. For all our composite parts,
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                        the comprising basic parts were registered separately on the Registry, to allow future iGEM teams to
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                        use our constructs in new assemblies. All parts we shipped to the registry are sequence confirmed.</p>
  
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<p> During the 2018 Giant Jamboree, <a href="http://parts.igem.org/Part:BBa_K2812004">BBa_K2812004</a> was nominated by the jury for Best New Basic Part in the Overgrad competition. <a href="http://parts.igem.org/Part:BBa_K2812005">BBa_K2812005</a> was nominated by the jury for Best New Composite Part in the Overgrad competition.</p>
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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ADD PARTS
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</a>
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                    <table class="parts-table">
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                      <caption>All Parts</caption>
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                      <tr>
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                        <th>Fave Part</th>
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                        <th>Name</th>
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                        <th>Type</th>
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                        <th>Description</th>
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                        <th>Designers</th>
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                        <th>Length</th>
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                      </tr>
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                      <tr class="odd-tr">
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                        <td><span style="color:red">❤</span></td>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812000">BBa_K2812000</a></td>
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                        <td>Basic</td>
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                        <td>Carbohydrate-binding domain from MpIBP</td>
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                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
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                        <td>576</td>
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                      </tr>
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                      <tr class="even-tr">
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                        <td></td>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812001">BBa_K2812001</a></td>
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                        <td>Basic</td>
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                        <td>Coding sequence for trunctated Lysostaphin</td>
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                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
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                        <td>738</td>
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                      </tr>
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                      <tr class="odd-tr">
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                        <td></td>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812002">BBa_K2812002</a></td>
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                        <td>Basic</td>
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                        <td>pBAD-ara promoter - Arabinose inducible regulatory promoter </td>
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                        <td>Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls</td>
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                        <td>71</td>
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                      </tr>
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                      <tr  class="even-tr">
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                        <td></td>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812003">BBa_K2812003</a></td>
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                        <td>Composite</td>
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                        <td>Coding sequence for trunctated Lysostaphin regulated by T7-promoter</td>
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                        <td>Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls</td>
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                        <td>776</td>
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                      </tr>
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                      <tr class="odd-tr">
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                        <td><span style="color:red">❤</span></td>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812004">BBa_K2812004</a></td>
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                        <td>Basic</td>
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                        <td>Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA</td>
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                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
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                        <td>1458</td>
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                      </tr>
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                      <tr  class="even-tr">
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                        <td><span style="color:red">❤</span></td>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812005">BBa_K2812005</a></td>
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                        <td>Composite</td>
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                        <td>Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter</td>
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                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
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                        <td>1496</td>
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                      </tr>
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                      <tr class="odd-tr">
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                        <td></td>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812006">BBa_K2812006</a></td>
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                        <td>Basic</td>
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                        <td>Coding sequence for Pyocin S5 with HlyA and His6-tag </td>
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                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
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                        <td>2217</td>
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                      </tr>
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                      <tr  class="even-tr">
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                        <td></td>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812007">BBa_K2812007</a></td>
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                        <td>Composite</td>
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                        <td>Coding sequence for Pyosin S5 with HlyA and His6-tag regulated by AraC promoter</td>
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                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
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                        <td>2288</td>
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                      </tr>
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                    </table>
 
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<h3>Inspiration</h3>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
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<ul>
 
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
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<h3>What information do I need to start putting my parts on the Registry?</h3>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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</ul>
 
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<p>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
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<h3>Part Table </h3>
 
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
 
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<groupparts>iGEM18 TU-Eindhoven</groupparts>
 
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{{:Team:TU-Eindhoven/footer}}

Latest revision as of 19:39, 6 December 2018

Parts overview

To realize our designed parts, we gratefully made use of the IDT Synthesis offer, allowing easy and fast access to the desired DNA constructs. All parts are codon optimized for expression in E. coli, the workhorse of our project. For all our composite parts, the comprising basic parts were registered separately on the Registry, to allow future iGEM teams to use our constructs in new assemblies. All parts we shipped to the registry are sequence confirmed.

During the 2018 Giant Jamboree, BBa_K2812004 was nominated by the jury for Best New Basic Part in the Overgrad competition. BBa_K2812005 was nominated by the jury for Best New Composite Part in the Overgrad competition.

All Parts
Fave Part Name Type Description Designers Length
❤ BBa_K2812000 Basic Carbohydrate-binding domain from MpIBP Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 576
BBa_K2812001 Basic Coding sequence for trunctated Lysostaphin Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 738
BBa_K2812002 Basic pBAD-ara promoter - Arabinose inducible regulatory promoter Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls 71
BBa_K2812003 Composite Coding sequence for trunctated Lysostaphin regulated by T7-promoter Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls 776
❤ BBa_K2812004 Basic Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 1458
❤ BBa_K2812005 Composite Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 1496
BBa_K2812006 Basic Coding sequence for Pyocin S5 with HlyA and His6-tag Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 2217
BBa_K2812007 Composite Coding sequence for Pyosin S5 with HlyA and His6-tag regulated by AraC promoter Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 2288