Difference between revisions of "Team:BioMarvel"
Skysea10101 (Talk | contribs) |
|||
| (91 intermediate revisions by 3 users not shown) | |||
| Line 1: | Line 1: | ||
| − | + | {{BioMarvel}} | |
| − | + | <html> | |
| − | + | ||
| − | + | <body> | |
| − | + | <script type="text/javascript"> | |
| − | + | ||
| − | + | window.onload = function(){ | |
| − | + | rotateBanner = setInterval(function(){ | |
| − | + | var pos = document.getElementsByName("pos"); | |
| − | + | var size = pos.length; | |
| − | + | for(var i = 0; i < size; i++) { | |
| − | + | if(pos[i].checked) { | |
| − | < | + | var m = i+1; |
| − | + | if (m >= size) { | |
| − | + | m = 0; | |
| − | + | } | |
| − | + | pos[m].checked = true; | |
| − | + | pos[i].checked = false; | |
| − | + | break; | |
| − | + | } | |
| − | + | } | |
| − | + | }, 3600); | |
| − | + | } | |
| − | + | ||
| − | + | ||
| − | + | </script> | |
| − | + | ||
| − | + | <style> | |
| − | + | *{margin:0;padding:0;} | |
| − | + | ul,li{list-style:none;} | |
| − | + | .slideBox{ | |
| − | + | position: relative; | |
| − | + | width: 100% | |
| − | + | } | |
| − | + | .slideBox:before{ | |
| − | + | content: ""; | |
| − | + | display: block; | |
| − | + | padding-top: 39.8148%; | |
| − | + | } | |
| − | + | .slideBoxbox{ | |
| − | + | position: absolute; | |
| − | + | top: 0; | |
| − | + | right: 0; | |
| − | + | bottom: 0; | |
| − | + | left: 0; | |
| − | + | } | |
| − | + | #slide{ | |
| − | + | height:100%; | |
| − | + | width:100%; | |
| − | + | position:relative; | |
| − | + | overflow:hidden; | |
| − | + | } | |
| − | + | #slide ul{width:300%;height:100%;transition:1s;} | |
| − | + | #slide ul:after{content:"";display:block;clear:both;} | |
| − | + | #slide li{float:left;width:33.333%;height:100%;} | |
| − | + | #slide li:nth-child(1){ | |
| − | + | ||
| − | + | } | |
| − | + | #slide li:nth-child(2){ | |
| − | + | ||
| − | + | } | |
| − | + | #slide li:nth-child(3){ | |
| − | + | ||
| − | + | } | |
| − | + | #slide input{display:none;} | |
| − | + | #slide label{display:inline-block;vertical-align:middle;width:15px;height:15px;border:2px solid #666;background:#fff;transition:0.3s;border-radius:50%;cursor:pointer;margin-left: : 5px;margin-right: 5px;} | |
| − | + | #slide .pos{text-align:center;position:absolute;bottom:10px;left:0;width:100%;text-align:center;} | |
| − | + | #pos1:checked~ul{margin-left:0%;} | |
| − | + | #pos2:checked~ul{margin-left:-100%;} | |
| − | + | #pos3:checked~ul{margin-left:-200%;} | |
| − | + | #pos1:checked~.pos>label:nth-child(1){background:#666;} | |
| − | + | #pos2:checked~.pos>label:nth-child(2){background:#666;} | |
| − | + | #pos3:checked~.pos>label:nth-child(3){background:#666;} | |
| − | + | ||
| − | + | .mSHdiv{ | |
| − | + | width: 100%; | |
| − | + | height: 45px; | |
| − | + | background-color: #203864; | |
| − | <div class=" | + | } |
| − | + | .mSHdiv h1{ | |
| − | + | color: white; | |
| − | + | margin-left: 10px; | |
| − | + | } | |
| − | + | .youtubeBox{ | |
| − | + | position: relative; | |
| − | < | + | display: block; |
| − | + | margin-left: auto; | |
| − | + | margin-right: auto; | |
| − | + | width: 94% | |
| − | + | } | |
| − | + | .youtubeBox:before{ | |
| − | + | content: ""; | |
| − | + | display: block; | |
| − | + | padding-top: 56.25%; | |
| − | + | } | |
| − | + | .youtubeBoxbox{ | |
| − | + | position: absolute; | |
| − | + | border: 2px solid black; | |
| − | + | top: 0; | |
| − | + | right: 0; | |
| − | + | bottom: 0; | |
| − | + | left: 0; | |
| − | + | } | |
| − | + | ||
| − | + | ||
| − | + | </style> | |
| − | + | ||
| − | + | ||
| − | + | <div class="mMainBox"> | |
| − | + | <section id="mSection"> | |
| − | + | <a href="https://www.facebook.com/biomarvel.igem.9"><img class="myimg" src="https://static.igem.org/mediawiki/2018/1/18/T--Biomarvel--mainhome.jpg"></a> | |
| − | + | </section> | |
| − | + | <div class="slideBox"> | |
| − | + | <div class="slideBoxbox"> | |
| − | + | <div id="slide"> | |
| − | + | <input type="radio" name="pos" id="pos1" checked> | |
| − | + | <input type="radio" name="pos" id="pos2"> | |
| − | + | <input type="radio" name="pos" id="pos3"> | |
| − | + | <ul> | |
| − | + | <li><a href="https://2018.igem.org/Team:BioMarvel/WET_LAB/Experiments"><img src="https://static.igem.org/mediawiki/2018/f/f0/T--Biomarvel--Banner1.png" style="width: 100%; height: 100%"></a></li> | |
| − | + | <li><a href="https://2018.igem.org/Team:BioMarvel/Public_Engagement"><img src="https://static.igem.org/mediawiki/2018/7/76/T--Biomarvel--Banner2_1.png" style="width: 100%; height: 100%"></a></li> | |
| − | + | <li><a href="https://2018.igem.org/Team:BioMarvel/Project/Description"><img src="https://static.igem.org/mediawiki/2018/7/7f/T--Biomarvel--Banner3.png" style="width: 100%; height: 100%"></a></li> | |
| − | + | </ul> | |
| − | + | <p class="pos" style="padding-top: 2em;padding-bottom: 1em; margin: 0;"> | |
| − | + | <label for="pos1"></label> | |
| − | < | + | <label for="pos2"></label> |
| + | <label for="pos3"></label> | ||
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | |||
</div> | </div> | ||
| − | < | + | <section id="mSection"> |
| − | <div class=" | + | <div class="mSHdiv"> |
| − | < | + | <h1 style="color: white;padding-top: 8px; padding-bottom: 7px;">Abstract</h1> |
</div> | </div> | ||
| − | + | <br> | |
| − | + | <p>The goal of this project is to construct a novel fusion protein of gold binding polypeptides (GBP)-protein G (ProG) to develop an electrochemical biosensor for rapid and simple diagnosis and monitoring heart failure.</p> | |
| − | + | <p>DH5-alpha <i>E. coli</i> strain was transformed by a genetically modified recombinant vector coding GBP and ProG. The GBP-ProG fusion protein was derived from the strain with IPTG-induced expression and purified using the TALON metal affinity resin. The resulting GBP-ProG was directly self-immobilized onto gold surfaces via the GBP portion, followed by the oriented binding of antibodies onto the ProG domain targeting the Fc region of antibodies.</p> | |
| − | + | <p>An electrochemical immunochip was fabricated through the GBP-ProG and gold patterned interdigitated array electrode. Antibody immobilization onto the gold surface of the electrode by the GBP-ProG was rapidly and simply achieved with proper antibody orientation. This immunochip could be used for diagnosis and monitoring of heart failure.</p> | |
| − | + | </section> | |
| − | + | <br> | |
| − | + | ||
| − | + | <section id="mSection"> | |
| − | + | <div class="mSHdiv"> | |
| − | + | <h1 style="color: white;padding-top: 8px; padding-bottom: 7px;">U-HEALTHCARE SERVICE SYSTEM</h1> | |
| − | < | + | |
</div> | </div> | ||
| − | + | <video class="myVideo" src="https://static.igem.org/mediawiki/2018/6/63/T--Biomarvel--Ubiquitous_System.mp4" muted="muted" controls="" autoplay="autoplay" loop="loop"></video> | |
| − | + | <br> | |
| − | < | + | |
| − | </section> | + | <!-- |
| − | < | + | <h1 style="text-align: center; text-shadow: 2px 2px 3px gray;">Ubiquitous System for Diagnosis and Monitoring of Heart Failure</h1> |
| − | </ | + | --> |
| − | </ | + | <br> |
| + | <a href="https://2018.igem.org/Team:BioMarvel/People/Team"><img class="myimg" src="https://static.igem.org/mediawiki/2018/2/27/T--Biomarvel--mainhome2_1.png"></a> | ||
| + | </section> | ||
| + | <br> | ||
| + | <section> | ||
| + | |||
| + | <div class="youtubeBox"> | ||
| + | <div class="youtubeBoxbox"> | ||
| + | <iframe width="100%" height="100%" src="https://www.youtube.com/embed/URUYaF4gsQ4" frameborder="0" allow="autoplay; encrypted-media" allowfullscreen></iframe> | ||
| + | </div> | ||
| + | </div> | ||
| + | </section> | ||
| + | |||
| + | </div> | ||
</body> | </body> | ||
| + | </html> | ||
| + | {{BioMarvel/footer}} | ||
Latest revision as of 23:28, 6 December 2018
Abstract
The goal of this project is to construct a novel fusion protein of gold binding polypeptides (GBP)-protein G (ProG) to develop an electrochemical biosensor for rapid and simple diagnosis and monitoring heart failure.
DH5-alpha E. coli strain was transformed by a genetically modified recombinant vector coding GBP and ProG. The GBP-ProG fusion protein was derived from the strain with IPTG-induced expression and purified using the TALON metal affinity resin. The resulting GBP-ProG was directly self-immobilized onto gold surfaces via the GBP portion, followed by the oriented binding of antibodies onto the ProG domain targeting the Fc region of antibodies.
An electrochemical immunochip was fabricated through the GBP-ProG and gold patterned interdigitated array electrode. Antibody immobilization onto the gold surface of the electrode by the GBP-ProG was rapidly and simply achieved with proper antibody orientation. This immunochip could be used for diagnosis and monitoring of heart failure.
U-HEALTHCARE SERVICE SYSTEM
Copyright © 2018 By BioMarvel. All right reserved.