Difference between revisions of "Team:BioMarvel"

 
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{{BioMarvel}}
 
{{BioMarvel}}
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                                <h2 class="maintitle">ORDINARY DESIGN FOR YOU</h2>
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                                <p class="subtitle">좋은 디자인이 필요한 프로젝트에 함께합니다</p>
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<main id="main" class="site-main" role="main">
 
  
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</header><!-- .entry-header -->
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<div class="entry-content">
 
<div id="pl-209"><div class="panel-grid" id="pg-209-0" ><div style="padding: 2%;padding: 2% 0; " data-overlay="true" class="panel-row-style" ><div class="panel-grid-cell" id="pgc-209-0-0" ><div class="so-panel widget widget_sow-hero panel-first-child" id="panel-209-0-0-0" data-index="0"><div style="text-align: left;" data-title-color="#443f3f" data-headings-color="#443f3f" class="panel-widget-style" ><div class="so-widget-sow-hero so-widget-sow-hero-default-62f1e4bb8844 so-widget-fittext-wrapper"><div class="sow-slider-base " style="display: none"><ul class="sow-slider-images" data-settings="{&quot;pagination&quot;:true,&quot;speed&quot;:800,&quot;timeout&quot;:8000,&quot;swipe&quot;:true}"> <li class="sow-slider-image  sow-slider-image-cover" style="background-color: #333333" >
 
<div class="sow-slider-image-container">
 
<div class="sow-slider-image-wrapper">
 
<h1 style="text-align: right"><span style="color: #ffffff">EMPATHY</span></h1><h1 style="text-align: right"><span style="color: #ffffff"> </span></h1> </div>
 
</div>
 
<div class="sow-slider-image-overlay sow-slider-image-cover" style="background-image: url(http://iwantdesign.co.kr/wp-content/uploads/2016/08/dont-think-about-it.jpg);opacity: 0.5"  ></div> </li>
 
</ul> <ol class="sow-slider-pagination">
 
<li><a href="#" data-goto="0">1</a></li>
 
</ol>
 
  
<div class="sow-slide-nav sow-slide-nav-next">
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<section id="mSection">
<em class="sow-sld-icon-thin-right"></em>
+
<a href="https://www.facebook.com/biomarvel.igem.9"><img class="myimg" src="https://static.igem.org/mediawiki/2018/1/18/T--Biomarvel--mainhome.jpg"></a>
</a>
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<ul>
 +
<li><a href="https://2018.igem.org/Team:BioMarvel/WET_LAB/Experiments"><img src="https://static.igem.org/mediawiki/2018/f/f0/T--Biomarvel--Banner1.png" style="width: 100%; height: 100%"></a></li>
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 +
<li><a href="https://2018.igem.org/Team:BioMarvel/Project/Description"><img src="https://static.igem.org/mediawiki/2018/7/7f/T--Biomarvel--Banner3.png" style="width: 100%; height: 100%"></a></li>
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<div class="sow-slide-nav sow-slide-nav-prev">
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<a href="#" data-goto="previous" data-action="prev">
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<em class="sow-sld-icon-thin-left"></em>
+
</a>
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</div>
+
</div></div></div></div><div class="so-panel widget widget_sow-editor panel-last-child" id="panel-209-0-0-1" data-index="1"><div style="text-align: left;" data-title-color="#443f3f" data-headings-color="#443f3f" class="panel-widget-style" ><div class="so-widget-sow-editor so-widget-sow-editor-base">
+
<div class="siteorigin-widget-tinymce textwidget">
+
<p style="text-align: left;"> </p>
+
<p style="text-align: left;">일상적 디자인은 디자인의 제 1순위로 공감을 지향합니다. 현대의 트렌드 / 클라이언트와의 충분한 커뮤니케이션을 통해 만족하실만한 아웃풋을 제공해 드립니다.</p>
+
<p style="text-align: left;"><span style="color: #f46455;"><em><strong> </strong></em></span></p>
+
</div>
+
</div></div></div></div><div class="panel-grid-cell" id="pgc-209-0-1" ><div class="so-panel widget widget_sow-hero panel-first-child" id="panel-209-0-1-0" data-index="2"><div style="text-align: left;" data-title-color="#443f3f" data-headings-color="#443f3f" class="panel-widget-style" ><div class="so-widget-sow-hero so-widget-sow-hero-default-2a33518813f4 so-widget-fittext-wrapper"><div class="sow-slider-base " style="display: none"><ul class="sow-slider-images" data-settings="{&quot;pagination&quot;:true,&quot;speed&quot;:800,&quot;timeout&quot;:8000,&quot;swipe&quot;:true}"> <li class="sow-slider-image  sow-slider-image-cover" style="background-color: #333333" >
+
<div class="sow-slider-image-container">
+
<div class="sow-slider-image-wrapper">
+
<h1 style="text-align: center"><span style="color: #ffffff">COMMUNICATION</span></h1>
+
<h1 style="text-align: right"><span style="color: #ffffff"> </span></h1> </div>
+
 
</div>
 
</div>
<div class="sow-slider-image-overlay sow-slider-image-cover" style="background-image: url(http://iwantdesign.co.kr/wp-content/uploads/2016/08/2.jpg);opacity: 0.5" ></div> </li>
+
<section id="mSection">
</ul> <ol class="sow-slider-pagination">
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<div class="mSHdiv">
<li><a href="#" data-goto="0">1</a></li>
+
<h1 style="color: white;padding-top: 8px; padding-bottom: 7px;">Abstract</h1>
</ol>
+
</div>
 +
<br>
 +
<p>The goal of this project is to construct a novel fusion protein of gold binding polypeptides (GBP)-protein G (ProG) to develop an electrochemical biosensor for rapid and simple diagnosis and monitoring heart failure.</p>
 +
<p>DH5-alpha <i>E. coli</i> strain was transformed by a genetically modified recombinant vector coding GBP and ProG. The GBP-ProG fusion protein was derived from the strain with IPTG-induced expression and purified using the TALON metal affinity resin. The resulting GBP-ProG was directly self-immobilized onto gold surfaces via the GBP portion, followed by the oriented binding of antibodies onto the ProG domain targeting the Fc region of antibodies.</p>
 +
<p>An electrochemical immunochip was fabricated through the GBP-ProG and gold patterned interdigitated array electrode. Antibody immobilization onto the gold surface of the electrode by the GBP-ProG was rapidly and simply achieved with proper antibody orientation. This immunochip could be used for diagnosis and monitoring of heart failure.</p>
 +
</section>
 +
<br>
  
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<h1 style="color: white;padding-top: 8px; padding-bottom: 7px;">U-HEALTHCARE SERVICE SYSTEM</h1>
</a>
+
</div>
</div>
+
<video class="myVideo" src="https://static.igem.org/mediawiki/2018/6/63/T--Biomarvel--Ubiquitous_System.mp4" muted="muted" controls="" autoplay="autoplay" loop="loop"></video>
 +
<br>
  
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<!--
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<h1 style="text-align: center; text-shadow: 2px 2px 3px gray;">Ubiquitous System for Diagnosis and Monitoring of Heart Failure</h1>
<em class="sow-sld-icon-thin-left"></em>
+
-->
</a>
+
<br>
 +
<a href="https://2018.igem.org/Team:BioMarvel/People/Team"><img class="myimg" src="https://static.igem.org/mediawiki/2018/2/27/T--Biomarvel--mainhome2_1.png"></a>
 +
</section>
 +
<br>
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<section>
 +
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<div class="youtubeBox">
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Latest revision as of 23:28, 6 December 2018

Abstract


The goal of this project is to construct a novel fusion protein of gold binding polypeptides (GBP)-protein G (ProG) to develop an electrochemical biosensor for rapid and simple diagnosis and monitoring heart failure.

DH5-alpha E. coli strain was transformed by a genetically modified recombinant vector coding GBP and ProG. The GBP-ProG fusion protein was derived from the strain with IPTG-induced expression and purified using the TALON metal affinity resin. The resulting GBP-ProG was directly self-immobilized onto gold surfaces via the GBP portion, followed by the oriented binding of antibodies onto the ProG domain targeting the Fc region of antibodies.

An electrochemical immunochip was fabricated through the GBP-ProG and gold patterned interdigitated array electrode. Antibody immobilization onto the gold surface of the electrode by the GBP-ProG was rapidly and simply achieved with proper antibody orientation. This immunochip could be used for diagnosis and monitoring of heart failure.


U-HEALTHCARE SERVICE SYSTEM




#iGEM #2018 #BioMarvel #Heart #Biochip #Diagnosis

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