Difference between revisions of "Team:BioMarvel"

 
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{{BioMarvel}}
 
{{BioMarvel}}
 
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                NANOTRAP: A two-pronged approach to preventing nanoparticle pollution in wastewater systems
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                Constructing DNA to make our ideas come to life. Click to EXPlore!
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                Computational Biology provides us insight on how to apply experimental data to real world applications!
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                It’s not only what happens in the lab, but also what happens in our community.
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                Meet the team, the faces behind NANOTRAP.
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                Attributions
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                Thank you to all those who helped and supported us.
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        <h1>NANOTRAP</h1>
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        <h6 id="abstract1">Nanoparticle Removal from Wastewater Systems</h6>
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        <h6 id="this_title">TAS_TAIPEI</h6>
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        <h6 id="this_title_2">2017 High School Grand Prize Winner</h6>
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                <h1 class="name col-lg-12">ABSTRACT</h1>
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                <h4 class="para col-lg-12">
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                  The small size of nanoparticles is both an advantage and a problem. Their high surface-area-to-volume ratio enables novel medical, industrial, and commercial applications. However, their small size also allows them to evade conventional filtration during water treatment, posing health risks to humans, plants, and aquatic life. Our project aims to remove nanoparticles using two approaches: 1) bind citrate-capped nanoparticles with the membrane protein proteorhodopsin and 2) trap nanoparticles using E. coli biofilm produced by overexpressing two regulators -- OmpR234 and CsgD. We envision integrating our trapping system in both rural and urban wastewater treatment plants to efficiently capture all nanoparticles before treated water is released into the environment.<br><br>
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<h1 style="color: white;padding-top: 8px; padding-bottom: 7px;">Abstract</h1>
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<br>
 +
<p>The goal of this project is to construct a novel fusion protein of gold binding polypeptides (GBP)-protein G (ProG) to develop an electrochemical biosensor for rapid and simple diagnosis and monitoring heart failure.</p>
 +
<p>DH5-alpha <i>E. coli</i> strain was transformed by a genetically modified recombinant vector coding GBP and ProG. The GBP-ProG fusion protein was derived from the strain with IPTG-induced expression and purified using the TALON metal affinity resin. The resulting GBP-ProG was directly self-immobilized onto gold surfaces via the GBP portion, followed by the oriented binding of antibodies onto the ProG domain targeting the Fc region of antibodies.</p>
 +
<p>An electrochemical immunochip was fabricated through the GBP-ProG and gold patterned interdigitated array electrode. Antibody immobilization onto the gold surface of the electrode by the GBP-ProG was rapidly and simply achieved with proper antibody orientation. This immunochip could be used for diagnosis and monitoring of heart failure.</p>
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{{BioMarvel/footer}}

Latest revision as of 23:28, 6 December 2018

Abstract


The goal of this project is to construct a novel fusion protein of gold binding polypeptides (GBP)-protein G (ProG) to develop an electrochemical biosensor for rapid and simple diagnosis and monitoring heart failure.

DH5-alpha E. coli strain was transformed by a genetically modified recombinant vector coding GBP and ProG. The GBP-ProG fusion protein was derived from the strain with IPTG-induced expression and purified using the TALON metal affinity resin. The resulting GBP-ProG was directly self-immobilized onto gold surfaces via the GBP portion, followed by the oriented binding of antibodies onto the ProG domain targeting the Fc region of antibodies.

An electrochemical immunochip was fabricated through the GBP-ProG and gold patterned interdigitated array electrode. Antibody immobilization onto the gold surface of the electrode by the GBP-ProG was rapidly and simply achieved with proper antibody orientation. This immunochip could be used for diagnosis and monitoring of heart failure.


U-HEALTHCARE SERVICE SYSTEM




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