Difference between revisions of "Team:NYMU-Taipei/Safety"

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<h1 style="color:#638695; font-size:70px; line-height:1em;"> Safety</h1>
  
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<h2 id="1">OVERVIEW</h2>
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<h1> Safety </h1>
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<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
  
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<h2 id="1">OVERVIEW</h2>
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<h3>Safe Project Design</h3>
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<h2 id="1">OVERVIEW</h2>
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<p ></p>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
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<li>Choosing a non-pathogenic chassis</li>
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<h2 id="1">OVERVIEW</h2>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<h2 class="story-title subtitle"></h2>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<p >This year, we proposed a quick and easy platform to determine the effectiveness of hair loss product without animal or human testing by evaluating a critical protein, DKK1, of the hair regeneration pathway. We are aware of the fact that all biological experiments could pose some risk to the environment and experimenter, and should only proceed under well regulation. We strictly follow a high standard of biosafety and responsible biological engineering of the guideline from iGEM Headquarter.</p>
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<h2 id="2">BIOSAFETY</h2>
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<h2 class="story-title subtitle"></h2>
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<p>The chassis we used in the project include Escherichia coli, Human cell line HEK293 and Dermal papilla. </p>
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<h3>Escherichia coli</h3>
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<p >We used Escherichia coli DH5alpha and BL21 in our project. Both of them are BSL-1 organism. Although the E. coli strains we use pose little or no risk of pathogenic, there's still risk of causing skin, eye, and respiratory tract irritation. To prevent these, everyone in wet-lab are required to wear full personal protection gear properly when operating with E. coli competent cells and cultures. All materials that came in contact with bacterial cultures or competent cells were autoclaved and disposed properly.</p>
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<h3>Human cell line</h3>
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<p >The cell line we used, HEK293, is BSL-1 organism, and dermal papilla, belong to BSL-2. All the experiments with human cell line are done in another BSL-2 cell culture room under the supervision of our instructor. All materials that came in contact with human cell lines were autoclaved and disposed of properly.</p>
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<h2 id="3">LAB SAFETY</h2>
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<h3>Training</h3>
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<p >To conduct experiments properly and safely, all of the team member should go through lab safety lesson and lab training directed by our instructors and member from 2017 NYMU_Taipei iGEM team.</p>
 
<h3>Safe Lab Work</h3>
 
<h3>Safe Lab Work</h3>
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<p >All experiments are done in proper condition considering its Biosafety Level and we strictly follow the Laboratory Safe Hygiene Precautions of our school. All team members participate in lab work wear personal protection equipment such as but not limited to lab coat, rubber gloves, long pants, and shoes of full coverage. All experimental procedure were checked and supervised by our instructor before we conducted, and Lab notes were record right after the experiment.</p>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h3>Waste Treatment</h3>
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<p >We gather chemical and bacteria mixture into a bottle containing bleach after conducting the experiments, and sterilize all trash with autoclave and discard it at a specific area of contaminative trash. All of these strictly follow <a href="https://ces.ym.edu.tw/files/11-1151-68.php">the Biological waste treatment process of Center of Environmental Protection and Safety and Health in our school.</a></p>
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<h3>Safe Shipment</h3>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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<p>We strictly ensured the implementation of all of these methods to prevent accidents, contamination and the spread of possible pathogens.</p>
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Revision as of 03:05, 26 September 2018

HAIR TO STAY
TEAM
Team Members
Collaborations
PROJECT
Description
Design
Experiments & Results
Notebook
InterLab
Model
Demonstrate
Improve
Attributions
PARTS
Parts Overview
Basic Parts
Composite Parts
Part Collection
SAFETY
HUMAN PRACTICES
Human Practices
Education & Engagement
AWARDS
Applied Design
Entrepreneurship
Model
JUDGING FORM ⇗



Safety

OVERVIEW

OVERVIEW

OVERVIEW


OVERVIEW

<img src="" style="box-shadow: -20px -20px #4B727B;">

This year, we proposed a quick and easy platform to determine the effectiveness of hair loss product without animal or human testing by evaluating a critical protein, DKK1, of the hair regeneration pathway. We are aware of the fact that all biological experiments could pose some risk to the environment and experimenter, and should only proceed under well regulation. We strictly follow a high standard of biosafety and responsible biological engineering of the guideline from iGEM Headquarter.

BIOSAFETY

<img src="" style="box-shadow: -20px -20px #4B727B;">

The chassis we used in the project include Escherichia coli, Human cell line HEK293 and Dermal papilla.

Escherichia coli

We used Escherichia coli DH5alpha and BL21 in our project. Both of them are BSL-1 organism. Although the E. coli strains we use pose little or no risk of pathogenic, there's still risk of causing skin, eye, and respiratory tract irritation. To prevent these, everyone in wet-lab are required to wear full personal protection gear properly when operating with E. coli competent cells and cultures. All materials that came in contact with bacterial cultures or competent cells were autoclaved and disposed properly.

Human cell line

The cell line we used, HEK293, is BSL-1 organism, and dermal papilla, belong to BSL-2. All the experiments with human cell line are done in another BSL-2 cell culture room under the supervision of our instructor. All materials that came in contact with human cell lines were autoclaved and disposed of properly.

LAB SAFETY

<img src="" style="box-shadow: -20px -20px #4B727B;">

Training

To conduct experiments properly and safely, all of the team member should go through lab safety lesson and lab training directed by our instructors and member from 2017 NYMU_Taipei iGEM team.

Safe Lab Work

All experiments are done in proper condition considering its Biosafety Level and we strictly follow the Laboratory Safe Hygiene Precautions of our school. All team members participate in lab work wear personal protection equipment such as but not limited to lab coat, rubber gloves, long pants, and shoes of full coverage. All experimental procedure were checked and supervised by our instructor before we conducted, and Lab notes were record right after the experiment.

Waste Treatment

We gather chemical and bacteria mixture into a bottle containing bleach after conducting the experiments, and sterilize all trash with autoclave and discard it at a specific area of contaminative trash. All of these strictly follow <a href="https://ces.ym.edu.tw/files/11-1151-68.php">the Biological waste treatment process of Center of Environmental Protection and Safety and Health in our school.</a>

We strictly ensured the implementation of all of these methods to prevent accidents, contamination and the spread of possible pathogens.