The goal of this project is to construct a novel fusion protein of gold binding polypeptides (GBP)-protein G (ProG) to develop an electrochemical biosensor for rapid and simple diagnosis and monitoring heart failure.

DH5-alpha E. coli strain was transformed by a genetically modified recombinant vector coding GBP and ProG. The GBP-ProG fusion protein was derived from the strain with IPTG-induced expression and purified using the TALON metal affinity resin. The resulting GBP-ProG was directly self-immobilized onto gold surfaces via the GBP portion, followed by the oriented binding of antibodies onto the ProG domain targeting the Fc region of antibodies.