Difference between revisions of "Team:NAU-CHINA/Parts"
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Revision as of 14:15, 1 October 2018
anti-GFP-mnotch-TEV-NLS-pSB1C3 The anti-GFP-mnotch-tetR-VP64 of Fudan University was transformed into anti-GFP-mnotch-TEV, and the flag tag and NLS were added at the right end. Make it our cell surface receptor after transformation. 插图，细胞图、western bolt结果 Bxb1 integrase-pSB1C3 Full length sequence of humanized Bxb1 integrase, Bxb1-reporter-pSB1C3 Inserting a reporter gene in the middle of the Bxb1 integrase recombination site Bxb1-site-pSB1C3 Bxb1 integrase recombination site sequence PhiC31 integrase-pSB1C3 Full-length sequence of humanized PhiC31 integrase PhiC31-reporter-pSB1C3 Inserting a reporter gene in the middle of the PhiC31 integrase recombination site PhiC31-site-pSB1C3 PhiC31 integrase recombination site sequence TP901 integrase-pSB1C3 Humanized TP901 integrase full length sequence TP901 -reporter-pSB1C3 Inserting a reporter gene into the TP901 integrase recombination site TP901-site-pSB1C3 TP901 integrase recombination site sequence TetO-miniCMV-Bxb1-pSB1C3 The TetO sequence, SV40 NLS, flag tag, miniCMV promoter, Bxb1 integrase are sequentially linked and involved in intracellular signal processing for detecting the strength of the promoter and the effect of different recombinases on the system. 插细胞荧光图 TetO-EF1a-Bxb1-pSB1C3 The TetO sequence, SV40 NLS, flag tag, EF1a promoter, and Bxb1 integrase are ligated in sequence, and participate in intracellular signal processing to detect the effect of promoter strength and different recombinases on the system. 插细胞荧光图 TetO-miniCMV-Tp901-pSB1C3 The TetO sequence, SV40 NLS, flag tag, miniCMV promoter, and Tp901 integrase are ligated in sequence to participate in intracellular signal processing for detecting the strength of the promoter and the effects of different recombinases on the system. 插细胞荧光图 TetO-EF1a-Tp901-pSB1C3 The TetO sequence, SV40 NLS, flag tag, EF1a promoter, and Tp901 integrase are ligated in sequence, and participate in intracellular signal processing to detect the effect of promoter strength and different recombinases on the system. 插细胞荧光图 TetO-EF1a-PhiC31-pSB1C3 The TetO sequence, SV40 NLS, flag tag, EF1a promoter, and PhiC31 integrase are ligated in sequence, and participate in intracellular signal processing to detect the effect of promoter strength and different recombinases on the system. 插细胞荧光图 EF1a-EGFP-pSB1C3 The EF1a promoter and EGFP coding sequences were ligated in sequence to characterize promoter strength. 插细胞荧光图 miniCMV-EGFP-pSB1C3 The miniCMV promoter and the EGFP coding sequence were ligated in sequence for characterizing the promoter strength. 插细胞荧光图 Ubc-EGFP-pSB1C3 The Ubc promoter and EGFP coding sequences were ligated in sequence for characterizing promoter strength. 插细胞荧光图