Difference between revisions of "Team:GreatBay China/Improve"
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Revision as of 05:15, 16 October 2018
mCATNIP2018@outlook.com
@Igem2018C
Improvement
BBa_J23119 is a classical strong consensus promoter that has been widely used among the iGEM community. This year, we designed a transcription-activator-like effector (TALE) stabilised version of J23119, TALE2 sp6, which is included as a member of the TALE stabilised promoter family. (Part Collection)
Upon addition of a TALE2 genetic circuit, J23119 is engineered to become a core promoter.
We first add an up-element which is an insulating spacer to separate it from the upstream TALE, creating an intermediate promoter UP119. Then the sequence between -35 region and -10 region of UP119 was altered to make a binding site for TALE2.
We characterised J23119 and TALE2 sp6 with GFP expression to verify the stabilisation effect, and have proven that TALE2 sp6 has significantly improved the expression consistency than J23119.