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Revision as of 22:02, 16 October 2018
Our Improved Part
BBa_K2557001
The Peking iGEM 2017 team identified the properties of Bxb1 gp35(BBa_K2243012)in E. coli. We obtained the sequence of this part and requested the plasmid from them. Since our chassis organism is mammalian cell, we optimized the codon and then we identified BBa_K2557001 in detail in HEK 293T cells. Although Bxb1 works well in HEK 293T cells, we did not repeat the activity reported by Peking iGEM 2017 team in E. coli.
Characterization
Other Improved parts
We also improved other parts in our genetic circuit, such as BBa_K2557000 and BBa_K2557007.
BBa_K2557000 is improved from BBa_K2446055, which is the synNotch coding part designed by Fudan iGEM 2017 . BBa_K2557007 is the coding sequence of TP901 recombinase, designed by Peking iGEM 2017(BBa_K2243000).
This year's Improvement parts standard stipulates that comparative experiments must be conducted, but the two parts, BBa_K2557000 and BBa_K2557007, we did not characterize their original part, so we did not use them as an improvement part.
Improve
For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Registry. Please include a link to your improved part on this page.
Gold Medal Criterion #2
Standard Tracks: Create a new part that has a functional improvement upon an existing BioBrick part. The sequences of the new and existing parts must be different. You must perform experiments with both parts to demonstrate this improvement. Document the experimental characterization on the Part's Main Page on the Registry for both the existing and new parts. Both the new and existing Main Page of each Part’s Registry entry must reference each other. Submit a sample of the new part to the Registry.
The existing part must NOT be from your 2018 part number range and must be different from the part documented in bronze #4.
Special Tracks: Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.