Gene Design

National Taiwan University

Professor K.S. Tung

Institute of Molecular and Cellular Biology
National Taiwan University

He provided us constitutive type of promoter (ADH1 promoter), and auxotrophic marker (LEU2, URA3, TRP1).

Professor C.T. Huang

Department of Biochemical Science and Technology
National Taiwan University

He suggested using constitutive promoter without inducing by methanol. That is GAP promoter which is commonly used in P.pastoris. He also recommended that we could choose commercial pGAPZ A vector from Invitrogen.

Material Properties

ITRI (Industrial Technology Research Institute)

Dr. Cheng-Han Hsieh

(The fourth on the left, Dr. Hsieh)

Research Fellow - Lignin Analytics
He recommended that we have to control the LIGGREEN in micromolecular to make it have the melting point. He also gave us some directions for decomposing lignin-like material. The Bacillus subtilis may be our good choice.

Dr. Shu-Chuan Huang

(The fourth on the left, Dr. Huang)

Research Fellow – Material and Chemical Research
She recommended that we should refer to the ISO 9000, ASTM D6400, and so on. Then, she recommended that we should cut the material into small pieces, carry out industrial composting, and detect the decomposition ratio.

National Chung Hsing University

Prof. Yuan-Shing Perng

Department of Forestry
National Chung Hsing University

He helped us test whether the paper laminated with LIGGREEN can be separated into paper pulping and LIGGREEN. In the interview with professor Perng, he confirmed that a small amount of lignin mixed into the ordinary pulp will not affect the paper remaking process, maybe LIGGREEN can be separated, too. He taught us to operate the pulper, after 200 seconds of pulping and beating, LIGGREEN and the paper were indeed separated. This proved that LIGGREEN would not harm in the pulp recycling system.

Prof. Wen-Jau Lee

Department of Forestry
National Chung Hsing University

He told us that in the reaction forming LIGGREEN from monolignols bonding to oligomers, there are some bonding nodes would act. He suggested that the oligomers we produced were preferably two-dimensional chain systems, not three-dimensional, to have thermoplastic properties.

Recycling Fund Management Board

Meng-Erh Wu

Deputy executive secretary of Recycling Fund Management Board

He recommended that our products may need to consider the shelf life, because some paper containers such as milk carton are packaging beverage for a long time and the lining of them cannot be leach. And he mentioned that the government used the method of Administrative guidance and Subsidy incentive to try to improve the recovery yield.
However, some misunderstandings in the process led to false positives in the news, causing the public to throw paper containers into general garbage disposal.

Production Line

Bobsen Paper Corporation

Bobsen Paper Corporation is responsible for producing coated paper and assisting downstream manufacturers in creating creases on paper. After our fundraising session, Bobsen agreed with us to arrange a corporate visit. Let us visit the factory and equipment of the paper laminating and answer our doubts about the production line.

Recycling Program

Prof. Ping-Mu Shu

Department of Earth and Environmental Science
National Chung Cheng University

He told us the truth of recycling in Taiwan, he also told us our product would not influence the paper recycled system because the amount of our LIGGREEN is very low and we do not need to set a recycled spot of LIGGREEN cups.

Lian-Tai

Lian-Tai is the only manufacturer in Taiwan that can separate paper from plastic lining. He helped us understand Taiwan's current recycling environment and recycling issues, you can refer to more details in iHP.

Chung Hwa Pulp Corporation

Chung Hwa Pulp Corporation provided us simple paper pulping and making process, they also answered the raw materials of paper.

Enterpreneumship

Yong-Zhong Chen

CEO of THEFREEN BURGER

He made us think about how to conduct market analysis to better develop LIGGREEN. He also reminded us to investigate the status of similar industries in the existing market, in order to better construct the business model and strengthen the link between LOGGREEN and society.