Difference between revisions of "Team:CCU Taiwan/Composite Part"
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<p class="description"><strong>Part construction in pGAPZ A:</strong><br> | <p class="description"><strong>Part construction in pGAPZ A:</strong><br> | ||
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<div id="Composite2" class="polaroid"> | <div id="Composite2" class="polaroid"> | ||
<img src="https://static.igem.org/mediawiki/parts/f/f2/T--CCU_Taiwan--_pGAPZ_A_Lac1%2C_pGAPZ_A_Px16%2C_pGAPZ_A_Px18_Colony_PCR.jpeg" style="width:100%"> | <img src="https://static.igem.org/mediawiki/parts/f/f2/T--CCU_Taiwan--_pGAPZ_A_Lac1%2C_pGAPZ_A_Px16%2C_pGAPZ_A_Px18_Colony_PCR.jpeg" style="width:100%"> | ||
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</div><br><br> | </div><br><br> | ||
| + | <p class="description"><strong>Part construction in pGAPZ A:</strong><br> | ||
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<div id="Composite5" class="polaroid"> | <div id="Composite5" class="polaroid"> | ||
<img src="https://static.igem.org/mediawiki/parts/f/f2/T--CCU_Taiwan--_pGAPZ_A_Lac1%2C_pGAPZ_A_Px16%2C_pGAPZ_A_Px18_Colony_PCR.jpeg" style="width:100%"> | <img src="https://static.igem.org/mediawiki/parts/f/f2/T--CCU_Taiwan--_pGAPZ_A_Lac1%2C_pGAPZ_A_Px16%2C_pGAPZ_A_Px18_Colony_PCR.jpeg" style="width:100%"> | ||
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<p class="second">BBa_K2809031<br> | <p class="second">BBa_K2809031<br> | ||
peroxidase 16 | peroxidase 16 | ||
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</div><br> | </div><br> | ||
| + | <p class="description"><strong>Part construction in pGAPZ A:</strong><br> | ||
| + | <div class="row"> | ||
<div id="Composite8" class="polaroid"> | <div id="Composite8" class="polaroid"> | ||
<img src="https://static.igem.org/mediawiki/parts/f/f2/T--CCU_Taiwan--_pGAPZ_A_Lac1%2C_pGAPZ_A_Px16%2C_pGAPZ_A_Px18_Colony_PCR.jpeg" style="width:100%"> | <img src="https://static.igem.org/mediawiki/parts/f/f2/T--CCU_Taiwan--_pGAPZ_A_Lac1%2C_pGAPZ_A_Px16%2C_pGAPZ_A_Px18_Colony_PCR.jpeg" style="width:100%"> | ||
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</div> | </div> | ||
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<br> | <br> | ||
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Revision as of 18:19, 17 October 2018
COMPOSITE PART
BBa_K2809011
laccase 1
We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809010.
This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of laccase gene sequence, HA tag for Western Blot protein detection and 6xHis-tag for protein purification.
Figure 1: The schematic structure of gene design- Lac1.
Part construction in pGAPZ A:
Figure 2: PCR result.
Well1: Marker/ Well2, 3, 4: Lac1 Colony 1, 2, 3/ Well5, 6, 7: Px16 Colony 1, 2, 3/ Well8, 9, 10: Px18 Colony 1, 2, 3/ Well11: pGAPZ A Colony
Insert long after PCR — pGAPZ A_Px16 :1377 bp/ pGAPZ A_Px18 :1359 bp/ pGAPZ A_Lac1 :2124bp
Figure 3: Miniprep plasmid digest by EcoRI, PstI.
WWell 1: Marker/ Well2, 3, 4: Plasmid of Lac1 colony1, 2, 3 after EcoRI, AgeI digest/ Well5, 6, 7: Plasmid of Px16 colony1, 2, 3 after EcoRI, AgeI digest/ Well8, 9, 10: Plasmid of Px18 colony1, 2, 3 after EcoRI, AgeI digest
Vector long after digest — pGAPZ A: 2884bp
Insert long after digest — Px16: 1295bp/ Px18: 1277bp/ Lac1: 2042bp
BBa_K2809021
peroxidase 18
We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809020.
This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of peroxidase 18 gene sequence, V5-tag for Western Blot protein detection and 6xHis-tag for protein purification.
Figure 4: The schematic structure of gene design- Px18.
Part construction in pGAPZ A:
Figure 5: PCR result.
Well1: Marker/ Well2, 3, 4: Lac1 Colony 1, 2, 3/ Well5, 6, 7: Px16 Colony 1, 2, 3/ Well8, 9, 10: Px18 Colony 1, 2, 3/ Well11: pGAPZ A Colony
Insert long after PCR — pGAPZ A_Px16 :1377 bp/ pGAPZ A_Px18 :1359 bp/ pGAPZ A_Lac1 :2124bp
Figure 6: Miniprep plasmid digest by EcoRI, PstI.
Well 1: Marker/ Well2, 3, 4: Plasmid of Lac1 colony1, 2, 3 after EcoRI, AgeI digest/ Well5, 6, 7: Plasmid of Px16 colony1, 2, 3 after EcoRI, AgeI digest/ Well8, 9, 10: Plasmid of Px18 colony1, 2, 3 after EcoRI, AgeI digest
Vector long after digest — pGAPZ A: 2884bp
Insert long after digest — Px16: 1295bp/ Px18: 1277bp/ Lac1: 2042bp
BBa_K2809031
peroxidase 16
We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809030.
This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of peroxidase 16 gene sequence, FLAG-tag for Western Blot protein detection and 6xHis-tag for protein purification.
Figure 7: The schematic structure of gene design- Px16.
Part construction in pGAPZ A:
Figure 8: PCR result.
Well1: Marker/ Well2, 3, 4: Lac1 Colony 1, 2, 3/ Well5, 6, 7: Px16 Colony 1, 2, 3/ Well8, 9, 10: Px18 Colony 1, 2, 3/ Well11: pGAPZ A Colony
Insert long after PCR — pGAPZ A_Px16 :1377 bp/ pGAPZ A_Px18 :1359 bp/ pGAPZ A_Lac1 :2124bp
Figure 9: Miniprep plasmid digest by EcoRI, PstI.
Well 1: Marker/ Well2, 3, 4: Plasmid of Lac1 colony1, 2, 3 after EcoRI, AgeI digest/ Well5, 6, 7: Plasmid of Px16 colony1, 2, 3 after EcoRI, AgeI digest/ Well8, 9, 10: Plasmid of Px18 colony1, 2, 3 after EcoRI, AgeI digest
Vector long after digest — pGAPZ A: 2884bp
Insert long after digest — Px16: 1295bp/ Px18: 1277bp/ Lac1: 2042bp