Yeasts & Enzymes

  In terms of gene design, our goal is mainly the production of plant enzymes. Therefore, we demonstrated the growth curve of the yeast and confirmed the enzyme production.

Figure1: Zeocin^TM selection

His4 experiment

  In our experiments, we have successfully constructed pGAPZ A containing HIS4 vectors. As of yet, we have just successfully transformed pGAPZ A+HIS4+Px18 vector into P.pastoris. In the future, we will do some further researches on this experiment.

Figure2: Plasmid digest (EcoRI, Agel) after miniprep

Figure3: Plasmid digest(SalI) check

Figure4: pGAPZ A+HIS4+Px18 selection with SD plate

Properties analysis

  For our production methods, we refer to the current material production and get some engineering conditions from the company. Through these engineering conditions, we have designed some modeling and production line. We hope that Liggreen will be more in line with business needs. We hope that Liggreen will have a place in the market.
  In terms of engineering properties of Liggreen, we also completed two important parts.

PART 1.  Modeling

Spontaneous reaction of monolignols & calculation of Flory-Stockmayer Theory of polymerization

  In our modeling, we constructed a theoretical calculation for our material. Based on published literature, we used Spartan to determine the spontaneous basis for our bond reaction, and then Flory-Stockmayer theory to provide a theoretical estimate of the polymerization. Through the establishment of the model, we can get theoretical data on Liggreen. In patent applications and product improvements, we can improve on this estimate through theoretical calculations and material measurements.

Figure5: Three Bonds based on Spartan Analysis

PART 2.  Structure analysis

  We analyzed the structure of our product. By UV-Visible we proved that the reaction has occurred and the linkage in our product is what we have expected. Also form other analysis, we learned more about the characteristics of our products.

PART 3.  Production line

  We have designed a production line to produce Liggreen. Using existing fermentation tank systems and reaction tank assemblies on the market, we can construct our production line.

Figure6: Production line

Market analysis

  We have already done a market survey of Liggreen. In response to the needs of the commodity market, we have discussed several practical business models and various analyses. Furthermore, we have visited several companies that produce laminated paper cups, as well as several companies that are concerned about new environmentally friendly materials. After these visits, our project has been further improved and more in line with social needs.

  In the future, we will make Liggreen not only in the laboratory but also meet the production standards of industry. We will promote the products to the public and fulfill the concept of environmental protection.