Laboratory notebooks

Our project aims to produce new material— LIGGREEN. In our experiment, we select P.pastoris as our strain to secrete enzymes which helps the polymerization of coniferyl alcohol. Besides, we divide into two groups to conduct experiment— gene sequence with or without Zeocin.

Here are the common steps of our experiment：
1. Binding our gene sequence by the transformation of E.coli
2. Extracting and purifying DNA purification from E.coli
3. Transforming the synthetic DNA into P.pastoris
4. Conducting western blot to detect our target enzyme
5. Purified target enzyme