Difference between revisions of "Team:CCU Taiwan/Materials"
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<td>Standard Minimal Medium (SD)</td> | <td>Standard Minimal Medium (SD)</td> | ||
<td>Yeast Nitrogen Base amino acids 6.7g<br> | <td>Yeast Nitrogen Base amino acids 6.7g<br> | ||
| − | H<sub>2</sub>O | + | H<sub>2</sub>O 850ml<br> |
50% Glucose 40ml<br> | 50% Glucose 40ml<br> | ||
| − | + | 10×-Leu/-Trp DO solution 100ml | |
</td> | </td> | ||
</tr> | </tr> | ||
Revision as of 03:21, 18 October 2018
MATERIALS
Antibiotics
| Name | Supplier |
| Zeocin™ (solution) | Thermo Fisher |
| Ampicillin | SIGMA |
| Chloramphenicol | SIGMA |
Antibody
| Name | Supplier |
| Anti-HA mouse Monoclonal | Yao-Hong Biotechnology |
| Anti-V5 mouse Monoclonal | Yao-Hong Biotechnology |
| Anti-FLAG mouse Monoclonal | Yao-Hong Biotechnology |
| Goat anti-Mouse IgG (H+L)-HRP | Leadgene Biomedical, Inc |
Enzyme
| Name | Supplier |
| BamHI | New England BioLabs |
| AseI | New England BioLabs |
| EcoRI | New England BioLabs |
| SalI | New England BioLabs |
| AgeI | New England BioLabs |
| PstI | New England BioLabs |
| AvrII | New England BioLabs |
Equipment
| Equipment | Name | Manufacturer |
| Table Centrifuge | Centrifuge 5415R | eppendorf |
| PCR-Machine | CYCLER-25 | major science |
| PCR-Machine | SimpliAmp™ Thermal Cycler | Life technology |
| UV light box | UV light box | TOPBIO |
| Digital gel image system | DigiGel DGIS-11c | TOPBIO |
| Water bath | Water bath B206 | FIRSTEK |
| Heat block | Dry bath incubator | major science |
| Scale | PB3002-S/PH DeltaRange | Mettler Toledo |
| NanoDrop | ND-1000 UV-Vis Spectrophotometer | Marshall Scientific |
| Hot plate | Corning® hotplate stirrers | SIBATA |
| Micro-computer compact ultra pure water system | Micro-computer compact ultra pure water system PPI-185UV | Uniss |
| Vortex mixer | Vortex-Genie 2 SI-0236 | Scientific Industries |
| Multiskan | Multiskan™ FC Microplate Photometer | Thermo |
Kits
| Name | Supplier |
| Pichia Expression Kit, original kit | Invitrogen™ |
| Miniprep | Biokit |
| Gel extraction | Biokit |
Markers
| Name | Supplier |
| 1 kb DNA marker XR-LYD008-1ML | YEASTERN BIOTECH |
| Omics RGB Prestained Protein Ladder | Omics Bio |
| DNA Gel Loading Dye (6X) | YEASTERN BIOTECH |
Media
| Media | Composition |
| LB medium | 10 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH2O |
| LB medium(low salt) | 5 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH2O |
| Yeast Extract–Peptone–Dextrose (YPD) Medium | 10 g yeast extract , 20 g peptone ,20g Dextrose in 1 L ddH2O |
| Standard Minimal Medium (SD) | Yeast Nitrogen Base amino acids 6.7g H2O 850ml 50% Glucose 40ml 10×-Leu/-Trp DO solution 100ml |