To realize our designed parts, we gratefully made use of the IDT Synthesis offer, allowing easy and fast access to the desired DNA constructs. All parts are codon optimized for expression in E. coli, the workhorse of our project. For all our composite parts, the comprising basic parts were registered separately on the Registry, to allow future iGEM teams to use our constructs in new assemblies. All parts we shipped to the registry are sequence confirmed. We nominated BBa_K2812004, lysostaphin-thrombin linker-HlyA-His tag, as Best New Basic Part as we think that the realisation of secretable lysostaphin opens up a whole new area of projects and applications, since the issues and limitations associated with cell lysis are avoided. The T7-Lysostaphin-thrombin linker-HlyA-His part, BBa_K2812005, has been nominated for Best New Composite Part, as it allows direct secretion of lysostaphin from the iGEM shipping backbone. This enables future teams to have a head start in their project.

Basic Parts

Fave Part	Name	Type	Description	Designers	Length
❤	BBa_K2812004	Basic	Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA	Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls	1458
❤	BBa_K2812000	Basic	Carbohydrate-binding domain from MpIBP	Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls	576
	BBa_K2812001	Basic	Coding sequence for trunctated Lysostaphin	Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls	738
	BBa_K2812002	Basic	pBAD-ara promoter - Arabinose inducible regulatory promoter	Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls	71
	BBa_K2812006	Basic	Coding sequence for Pyocin S5 with HlyA and His6-tag	Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls	2217