We used an efficient two day cloning cycle split into a "Light" day and a "Heavy" day.

Light Day

The light day consists of Colony PCR and liquid culture of colonies transformed from a previous day.

1. The 3-in-1

   First, count the number of colonies you want to check. Then, do the following 3 things sequentially:

   • Liquid culture
   • 2nd time plate
   • Colony PCR

   (Use the same tip to add the template to these three things)

2. Make The Gel For Electrophoresis
3. Run Gel Electrophoresis To Check The Colony PCR Product