Difference between revisions of "Team:BioMarvel"

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<h1>ABSTRACT</h1>
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<p>The goal of this project is to construct a novel fusion protein of gold binding polypeptides (GBP)-protein G (ProG) to develop an electrochemical biosensor for rapid and simple diagnosis and monitoring heart failure.</p><br>
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<p>DH5-alpha E. coli strain was transformed by a genetically modified recombinant vector coding GBP and ProG. The GBP-ProG fusion protein was derived from the strain with IPTG-induced expression and purified using the TALON metal affinity resin. The resulting GBP-ProG was directly self-immobilized onto gold surfaces via the GBP portion, followed by the oriented binding of antibodies onto the ProG domain targeting the Fc region of antibodies.</p>
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<h1>U-HEALTHCARE SERVICE SYSTEM</h1>
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<img class="myimg" src="https://static.igem.org/mediawiki/2018/a/a1/T--Biomarvel--uhealth.jpg">
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Revision as of 12:59, 30 September 2018

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ABSTRACT

The goal of this project is to construct a novel fusion protein of gold binding polypeptides (GBP)-protein G (ProG) to develop an electrochemical biosensor for rapid and simple diagnosis and monitoring heart failure.


DH5-alpha E. coli strain was transformed by a genetically modified recombinant vector coding GBP and ProG. The GBP-ProG fusion protein was derived from the strain with IPTG-induced expression and purified using the TALON metal affinity resin. The resulting GBP-ProG was directly self-immobilized onto gold surfaces via the GBP portion, followed by the oriented binding of antibodies onto the ProG domain targeting the Fc region of antibodies.

U-HEALTHCARE SERVICE SYSTEM

#iGEM #2018 #BioMarvel #Heart #Biochip #Diagnosis

Contact Us !

IGEM2018BioMarvel@gmail.com

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