Difference between revisions of "Team:BioMarvel"
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<h1>ABSTRACT</h1> | <h1>ABSTRACT</h1> | ||
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<p>The goal of this project is to construct a novel fusion protein of gold binding polypeptides (GBP)-protein G (ProG) to develop an electrochemical biosensor for rapid and simple diagnosis and monitoring heart failure.</p><br> | <p>The goal of this project is to construct a novel fusion protein of gold binding polypeptides (GBP)-protein G (ProG) to develop an electrochemical biosensor for rapid and simple diagnosis and monitoring heart failure.</p><br> | ||
<p>DH5-alpha E. coli strain was transformed by a genetically modified recombinant vector coding GBP and ProG. The GBP-ProG fusion protein was derived from the strain with IPTG-induced expression and purified using the TALON metal affinity resin. The resulting GBP-ProG was directly self-immobilized onto gold surfaces via the GBP portion, followed by the oriented binding of antibodies onto the ProG domain targeting the Fc region of antibodies.</p> | <p>DH5-alpha E. coli strain was transformed by a genetically modified recombinant vector coding GBP and ProG. The GBP-ProG fusion protein was derived from the strain with IPTG-induced expression and purified using the TALON metal affinity resin. The resulting GBP-ProG was directly self-immobilized onto gold surfaces via the GBP portion, followed by the oriented binding of antibodies onto the ProG domain targeting the Fc region of antibodies.</p> | ||
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<section id="mSection"> | <section id="mSection"> | ||
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<h1>U-HEALTHCARE SERVICE SYSTEM</h1> | <h1>U-HEALTHCARE SERVICE SYSTEM</h1> | ||
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<p></p> | <p></p> | ||
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<img class="myimg" src="https://static.igem.org/mediawiki/2018/a/a1/T--Biomarvel--uhealth.jpg"> | <img class="myimg" src="https://static.igem.org/mediawiki/2018/a/a1/T--Biomarvel--uhealth.jpg"> | ||
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<img class="myimg" src="https://static.igem.org/mediawiki/2018/d/d8/T--Biomarvel--mainhome2.jpg"> | <img class="myimg" src="https://static.igem.org/mediawiki/2018/d/d8/T--Biomarvel--mainhome2.jpg"> | ||
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Revision as of 13:01, 30 September 2018
ABSTRACT
The goal of this project is to construct a novel fusion protein of gold binding polypeptides (GBP)-protein G (ProG) to develop an electrochemical biosensor for rapid and simple diagnosis and monitoring heart failure.
DH5-alpha E. coli strain was transformed by a genetically modified recombinant vector coding GBP and ProG. The GBP-ProG fusion protein was derived from the strain with IPTG-induced expression and purified using the TALON metal affinity resin. The resulting GBP-ProG was directly self-immobilized onto gold surfaces via the GBP portion, followed by the oriented binding of antibodies onto the ProG domain targeting the Fc region of antibodies.
U-HEALTHCARE SERVICE SYSTEM
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