Difference between revisions of "Team:NYMU-Taipei/experimentsandresults"
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<h2>E.coli Protocols</h2> | <h2>E.coli Protocols</h2> | ||
<div class="protocols"> | <div class="protocols"> | ||
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<div id="1t" class="trigger" onclick="detail(event)">Two-day Efficient Cloning Cycle</div> | <div id="1t" class="trigger" onclick="detail(event)">Two-day Efficient Cloning Cycle</div> | ||
<div id="1d" class="detail"> | <div id="1d" class="detail"> | ||
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<div id="2t" class="trigger"onclick="detail(event)">Plasmid Extraction | <div id="2t" class="trigger"onclick="detail(event)">Plasmid Extraction | ||
<div id="2d" class="detail">ballalalla</div></div> | <div id="2d" class="detail">ballalalla</div></div> | ||
Revision as of 03:33, 1 October 2018
HAIR TO STAY
Protocols
E.coli Protocols
Two-day Efficient Cloning Cycle
We used an efficient two day cloning cycle split into a "Light" day and a "Heavy" day.
Light Day
The light day consists of Colony PCR and liquid culture of colonies transformed from a previous day.
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The 3-in-1
First, count the number of colonies you want to check. Then, do the following 3 things sequentially:
- Liquid culture
- 2nd time plate
- Colony PCR
(Use the same tip to add the template to these three things)
- Make The Gel For Electrophoresis
- Run Gel Electrophoresis To Check The Colony PCR Product
Plasmid Extraction
ballalalla
Plasmid Extraction
ballalalla
Plasmid Extraction
ballalalla