Difference between revisions of "Team:XMU-China/Composite Part"

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                     Composite Parts
 
                     Composite Parts
 
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                 <p class="indent">We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is <span><a class="click_here" href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a></span>, which links <span><a class="click_here" href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a></span> and <span><a class="click_here" href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a></span>, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatche.
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                 <p class="indent">We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is <a href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a>, which links <a href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> and <a href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a>, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatche.
 
                 </p>
 
                 </p>
 
                 <img src="https://static.igem.org/mediawiki/2018/1/14/T--XMU-China--Composite_Parts_table.png">
 
                 <img src="https://static.igem.org/mediawiki/2018/1/14/T--XMU-China--Composite_Parts_table.png">
 
                 <img src="https://static.igem.org/mediawiki/2018/9/96/T--XMU-China--Composite_Parts_table2.png">
 
                 <img src="https://static.igem.org/mediawiki/2018/9/96/T--XMU-China--Composite_Parts_table2.png">
 
                 <table class="table">
 
                 <table class="table">
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                     <tr>
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                     <tr><span><
 
                         <th>Favorite</th>
 
                         <th>Favorite</th>
 
                         <th>Register Name</th>
 
                         <th>Register Name</th>
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a></td>
 
                         <td>NG8-1</td>
 
                         <td>NG8-1</td>
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                         <td>A composite part linked by BBa_K2623022 and BBa_K2623025</td>
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                         <td>A composite part linked by <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> and <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a></td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>3410 bp</td>
 
                         <td>3410 bp</td>
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                         <td>Hongling Liu</td>
 
                         <td>Hongling Liu</td>
 
                     </tr>
 
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                     <tr>
 
                     <tr>
 
                         <td></td>
 
                         <td></td>
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623021">BBa_K2623021</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623021">BBa_K2623021</a></td>
 
                         <td>NG1</td>
 
                         <td>NG1</td>
−
                         <td>BBa_K2623022 with GFP at its N-termini.</td>
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                         <td><a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> with GFP at its N-termini.</td>
 
                         <td>Reporter</td>
 
                         <td>Reporter</td>
 
                         <td>1657 bp</td>
 
                         <td>1657 bp</td>
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a></td>
 
                         <td>NG4</td>
 
                         <td>NG4</td>
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                         <td>A non-GFP form of the BBa_K2623023</td>
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                         <td>A non-GFP form of the <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623023">BBa_K2623023</a></td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>898 bp</td>
 
                         <td>898 bp</td>
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                     <tr>
 
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                         <td></td>
 
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623029">BBa_K2623026</a></td>
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623029">BBa_K2623029</a></td>
 
                         <td>NG8-2</td>
 
                         <td>NG8-2</td>
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                         <td>A composite part linked by BBa_K2623022 and BBa_K2623026.</td>
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                         <td>A composite part linked by <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> and <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623026">BBa_K2623026</a>.</td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>2698 bp</td>
 
                         <td>2698 bp</td>
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623030">BBa_K2623030</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623030">BBa_K2623030</a></td>
 
                         <td>NG9-1</td>
 
                         <td>NG9-1</td>
−
                         <td>A composite part linked by BBa_K2623024 and BBa_K2623025.</td>
+
                         <td>A composite part linked by <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a> and <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a>.</td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>3410 bp</td>
 
                         <td>3410 bp</td>
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623031">BBa_K2623031</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623031">BBa_K2623031</a></td>
 
                         <td>NG9-2</td>
 
                         <td>NG9-2</td>
−
                         <td>A composite part linked by BBa_K2623024 and BBa_K2623026</td>
+
                         <td>A composite part linked by <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a> and <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623026">BBa_K2623026</a></td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>2698 bp</td>
 
                         <td>2698 bp</td>
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                         <td><a href="http://parts.igem.org/Part:BBa_K2623032">BBa_K2623032</a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2623032">BBa_K2623032</a></td>
 
                         <td>NG10</td>
 
                         <td>NG10</td>
−
                         <td>A composite part linked by BBa_K2623024, BBa_K2623026 and BBa_K2623027</td>
+
                         <td>A composite part linked by <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a>, BBa_K2623026 and <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623027">BBa_K2623027</a></td>
 
                         <td>Composite</td>
 
                         <td>Composite</td>
 
                         <td>4153 bp</td>
 
                         <td>4153 bp</td>

Revision as of 13:56, 12 October 2018

Team:XMU-China/Composite Parts - 2018.igem.org

Composite Parts
Composite Parts

We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is BBa_K2623028, which links BBa_K2623022 and BBa_K2623025, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatche.

<
Favorite Register Name Code used in Lab Description Types Lenth Designer
R BBa_K2623028 NG8-1 A composite part linked by BBa_K2623022 and BBa_K2623025 Composite 3410 bp Niangui Cai
BBa_K2623011 Kai 2 Phosphorylation pathsway builder of Kai Composite 4444 bp Gezhi Xiao
BBa_K2623013 Kai 1 3 Kai's phosphorylation core oscllator builder and SfYFP reportor for pKaiBC Composite 3978 bp Hongling Liu
BBa_K2623014 CAHS 1 Cytosolic-abundant heat soluble protein "CAHS " (promoter, RBS, RFP, lacI and double terminator) Composite 2891 bp Hongling Liu
BBa_K2623015 SAHS 1 Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, RFP and double terminator) Composite 1425 bp Jun Dai
BBa_K2623016 SAHS 2 Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, amilCP and double terminator) Composite 1431 bp Yuanjun Shang
BBa_K2623021 NG1 BBa_K2623022 with GFP at its N-termini. Reporter 1657 bp Niangui Cai
BBa_K2623022 NG2 Bacterial outer membrane protein A (OmpA) fused with SpyTag at its C-termini Composite 910 bp Qiupeng Wang
BBa_K2623023 NG3 Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini. Reporter 1645 bp Junhong Chen
BBa_K2623024 NG4 A non-GFP form of the BBa_K2623023 Composite 898 bp Jiyang Zheng
BBa_K2623025 NG5 Archaeal ribosomal protein protein L7Ae fused with SpyCatcher and mRFP1. Reporter 2613 bp Ruofan Yang
BBa_K2623026 NG6 Archaeal ribosomal protein protein L7Ae fused with SpyCatcher. Composite 2317 bp Yunyun Hu
BBa_K2623029 NG8-2 A composite part linked by BBa_K2623022 and BBa_K2623026. Composite 2698 bp Ye Qiang
BBa_K2623030 NG9-1 A composite part linked by BBa_K2623024 and BBa_K2623025. Composite 3410 bp Yang Liang
BBa_K2623031 NG9-2 A composite part linked by BBa_K2623024 and BBa_K2623026 Composite 2698 bp Junhong Chen
BBa_K2623032 NG10 A composite part linked by BBa_K2623024, BBa_K2623026 and BBa_K2623027 Composite 4153 bp Niangui Cai
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