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| | <div class="paragraphs"> | | <div class="paragraphs"> |
| | <p>This year, we constructed plenty of parts that can be categorized into 3 groups:</p> | | <p>This year, we constructed plenty of parts that can be categorized into 3 groups:</p> |
| − | <p>1. The plasmids for FRET Model</p> | + | <h2>1. The plasmids for FRET Model</h2> |
| | <p>This group ranges from <a>part BBa_K275001</a> to <a>BBa_K275007</a>. The parts are the ones that we primarily constructed and utilized in the FRET system. <a>BBa_K275001</a> is a pET32a with its MCS replaced with sequences designed by us. The replacement sequence contains a specifically designed MCS that can be inserted with a FRET protein and a DKK1-binding protein and express the two proteins as a fusion protein under the control of lac operon of pET32a. Hence, <a>part BBa_K275003</a> to <a>part BBa_K275007</a> are all cloned using BBa_K275001.</p> | | <p>This group ranges from <a>part BBa_K275001</a> to <a>BBa_K275007</a>. The parts are the ones that we primarily constructed and utilized in the FRET system. <a>BBa_K275001</a> is a pET32a with its MCS replaced with sequences designed by us. The replacement sequence contains a specifically designed MCS that can be inserted with a FRET protein and a DKK1-binding protein and express the two proteins as a fusion protein under the control of lac operon of pET32a. Hence, <a>part BBa_K275003</a> to <a>part BBa_K275007</a> are all cloned using BBa_K275001.</p> |
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