Difference between revisions of "Team:XJTLU-CHINA"

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                 <h1 style="font-size: 40px; font-family:'Impact'; color: #337ab7;">Exosome construct</h1>
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                 <h1 style="font-size: 40px; font-family:'Impact'; color: #337ab7;">RNA Packaging</h1>
 
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                    We also enabled the transfection and protein expression of extracellular mRNA in living organism’s neuron by using two mRNA modifications.
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                  Since exosomes have randomicity in substances packaging, we modified exosomes to be capable of packaging our designed therapeutic mRNA in larger amount. To achieve this goal, a RNA-protein interaction strategy was utilized.  
 
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                 <h1 style="font-size: 40px; font-family:'Impact'; color: #337ab7;">Exosome delivery</h1>
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                 <h1 style="font-size: 40px; font-family:'Impact'; color: #337ab7;">Exosome delivery and targeting</h1>
 
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                     Exosome is a kind of cell-derived vesicles with diameters between 30 nm ~100 nm and is naturally capable of passing the BBB. Our project involves three exosome device modification: RNA packaging, target cell characterization and exosome delivery.
 
                     Exosome is a kind of cell-derived vesicles with diameters between 30 nm ~100 nm and is naturally capable of passing the BBB. Our project involves three exosome device modification: RNA packaging, target cell characterization and exosome delivery.

Revision as of 16:04, 14 October 2018

Exosome biogenesis

This year, we decided to engineer human embryonic kidney cells (HEK293T) to produce engineered exosomes. we genetically modified HEK293T to increase the production of exosome.

RNA Packaging

Since exosomes have randomicity in substances packaging, we modified exosomes to be capable of packaging our designed therapeutic mRNA in larger amount. To achieve this goal, a RNA-protein interaction strategy was utilized.

Exosome delivery and targeting

Exosome is a kind of cell-derived vesicles with diameters between 30 nm ~100 nm and is naturally capable of passing the BBB. Our project involves three exosome device modification: RNA packaging, target cell characterization and exosome delivery.

Collaborators and Supporters

Location

Rm 363, Science Building

Xi'an Jiaotong-Liverpool University

111 Ren'ai Road, Suzhou, China

215123

Social

FB

Get in touch

igem@xjtlu.edu.cn