Difference between revisions of "Team:CCU Taiwan/Our Plan"

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<p class="description">&emsp;&emsp;We analyzed the lignin-like polymer we produced to confirm the work is successful. In the first stage of analysis, we started by analyzing the content and structure of our lignin-like polymer by UV-visible spectroscopy. For the second stage, we used NMR spectroscopy and Mass Spectroscopy to analyze information about the molecular structure and molecular weight of our polymer. In the last stage, Thermogravimetric analysis is used to analyze the melting or decomposition point of our product. After the above experiments are completed, we have a deeper understanding of the lignin-like polymer we have made. These experimental data also help us in the application and improvement of the products.</p>
 
<p class="description">&emsp;&emsp;We analyzed the lignin-like polymer we produced to confirm the work is successful. In the first stage of analysis, we started by analyzing the content and structure of our lignin-like polymer by UV-visible spectroscopy. For the second stage, we used NMR spectroscopy and Mass Spectroscopy to analyze information about the molecular structure and molecular weight of our polymer. In the last stage, Thermogravimetric analysis is used to analyze the melting or decomposition point of our product. After the above experiments are completed, we have a deeper understanding of the lignin-like polymer we have made. These experimental data also help us in the application and improvement of the products.</p>
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<p class="first">Reaction experiment</p><br>
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                <div id="Analysis1" class="polaroid" style="display:inline-block">
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                  <img src="https://static.igem.org/mediawiki/2018/2/20/T--CCU_Taiwan--CCUUVvisible1.jpg" width="100%">
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                  <div class="container">
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                    <p>Figure1: UV-Visible spectrum of products</p>
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                  </div>
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                </div>
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                <div id="Analysis2" class="polaroid" style="display:inline-block">
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                  <img src="https://static.igem.org/mediawiki/2018/4/4d/T--CCU_Taiwan--CCUconuferylNMR.png" width="100%">
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                  <div class="container">
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                    <p>Figure2: NMR Spectrum of coniferyl alcohol</p>
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                  </div>
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                </div>
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                <div id="Analysis3" class="polaroid" style="display:inline-block">
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                  <img src="https://static.igem.org/mediawiki/2018/7/7d/T--CCU_Taiwan--CCUligninNMR.jpg" width="100%">
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                  <div class="container">
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                    <p>Figure3: NMR Spectrum of commercial dealkaline lignin</p>
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                  </div>
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                </div>
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                <div id="Analysis4" class="polaroid" style="display:inline-block">
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                  <img src="https://static.igem.org/mediawiki/2018/9/9c/T--CCU_Taiwan--CCUlaccaseNMR.jpg" width="100%">
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                  <div class="container">
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                    <p>Figure4: NMR Spectrum of Laccase-involved reaction product</p>
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                  </div>
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                </div>
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                <div id="Analysis5" class="polaroid" style="display:inline-block">
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                  <img src="https://static.igem.org/mediawiki/2018/8/8f/T--CCU_Taiwan--CCUlaccaseandperoxidaseNMR.jpg" width="100%">
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                  <div class="container">
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                    <p>Figure5: NMR Spectrum of Laccase & Peroxidase-involved reaction product</p>
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                  </div>
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                </div>
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Revision as of 12:06, 16 October 2018

ANALYSIS



  We analyzed the lignin-like polymer we produced to confirm the work is successful. In the first stage of analysis, we started by analyzing the content and structure of our lignin-like polymer by UV-visible spectroscopy. For the second stage, we used NMR spectroscopy and Mass Spectroscopy to analyze information about the molecular structure and molecular weight of our polymer. In the last stage, Thermogravimetric analysis is used to analyze the melting or decomposition point of our product. After the above experiments are completed, we have a deeper understanding of the lignin-like polymer we have made. These experimental data also help us in the application and improvement of the products.



Reaction experiment


Figure1: UV-Visible spectrum of products

Figure2: NMR Spectrum of coniferyl alcohol

Figure3: NMR Spectrum of commercial dealkaline lignin

Figure4: NMR Spectrum of Laccase-involved reaction product

Figure5: NMR Spectrum of Laccase & Peroxidase-involved reaction product