Difference between revisions of "Team:CCU Taiwan/Demonstrate"

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<p class="first" id="ca2">His4 experiment</p>
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<p class="description"> &emsp;&emsp;In our experiments, we have successfully constructed pGAPZ A containing HIS4 vectors. As of yet, we have just successfully transformed pGAPZ A+HIS4+Px18 vector into P.pastoris. In the future, we will do some further researches on this experiment.</p>
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                <div id="Demonstrate2" class="polaroid">
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                  <img src="https://static.igem.org/mediawiki/2018/6/69/T--CCU_Taiwan--CCUDEMON2.png" style="width:100%">
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                  <div class="container">
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                    <p>Figure2: Plasmid digest (EcoRI, Agel) after miniprep</p>
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                  </div>
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                </div>
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                <div id="Demonstrate3" class="polaroid">
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                  <img src="https://static.igem.org/mediawiki/2018/4/45/T--CCU_Taiwan--133.png" style="width:100%">
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                  <div class="container">
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                    <p>Figure3: Plasmid digest(SalI) check</p>
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                  </div>
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                </div>
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                <div id="Demonstrate4" class="polaroid">
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                  <img src="https://static.igem.org/mediawiki/2018/8/80/T--CCU_Taiwan--134.png" style="width:100%">
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                  <div class="container">
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                    <p>Figure4: pGAPZ A+HIS4+Px18 selection with SD plate</p>
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                  </div>
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                </div>
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<p class="first" id="ca2">Properties analysis</p>
 
<p class="first" id="ca2">Properties analysis</p>
 
<p class="description"> &emsp;&emsp;For our production methods, we refer to the current material production and get some engineering conditions from the company. Through these engineering conditions, we have designed some modeling and production line. We hope that Liggreen will be more in line with business needs. We hope that Liggreen will have a place in the market.
 
<p class="description"> &emsp;&emsp;For our production methods, we refer to the current material production and get some engineering conditions from the company. Through these engineering conditions, we have designed some modeling and production line. We hope that Liggreen will be more in line with business needs. We hope that Liggreen will have a place in the market.
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<p class="description"> &emsp;&emsp;In our modeling, we constructed a theoretical calculation for our material. Based on published literature, we used Spartan to determine the spontaneous basis for our bond reaction, and then Flory-Stockmayer theory to provide a theoretical estimate of the polymerization. Through the establishment of the model, we can get theoretical data on Liggreen. In patent applications and product improvements, we can improve on this estimate through theoretical calculations and material measurements.</p>  
 
<p class="description"> &emsp;&emsp;In our modeling, we constructed a theoretical calculation for our material. Based on published literature, we used Spartan to determine the spontaneous basis for our bond reaction, and then Flory-Stockmayer theory to provide a theoretical estimate of the polymerization. Through the establishment of the model, we can get theoretical data on Liggreen. In patent applications and product improvements, we can improve on this estimate through theoretical calculations and material measurements.</p>  
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<div id="Demonstrate2" class="polaroid">
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<div id="Demonstrate5" class="polaroid">
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                   <img src="https://static.igem.org/mediawiki/2018/9/99/T--CCU_Taiwan--demonstrate-11.png" style="width:120%">
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                   <img src="https://static.igem.org/mediawiki/2018/2/28/T--CCU_Taiwan--CCUmodelbasicpsrt3.png" style="width:120%">
 
                   <div class="container">
 
                   <div class="container">
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                     <p>Figure2: Three Bonds based on Spartan Analysis</p>
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                     <p>Figure5: Three Bonds based on Spartan Analysis</p>
 
                   </div>
 
                   </div>
 
                 </div>
 
                 </div>
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<p class="description"> &emsp;&emsp;We have designed a production line to produce Liggreen. Using existing fermentation tank systems and reaction tank assemblies  on the market, we can construct our production line.</p>
 
<p class="description"> &emsp;&emsp;We have designed a production line to produce Liggreen. Using existing fermentation tank systems and reaction tank assemblies  on the market, we can construct our production line.</p>
 
<br>  <br>
 
<br>  <br>
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<p class="first" id="ca3">Market analysis</p>  <br>
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<div id="Demonstrate5" class="polaroid">
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<p class="description">&emsp;&emsp;We have already done a market survey of Liggreen. In response to the needs of the commodity market, we have discussed several practical business models and various analyses. Furthermore, we have visited several companies that produce laminated paper cups, as well as several companies that are concerned about new environmentally friendly materials. After these visits, our project has been further improved and more in line with social needs.</p>
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                   <img src="https://static.igem.org/mediawiki/2018/2/28/T--CCU_Taiwan--CCUmodelbasicpsrt3.png" style="width:120%">
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<div id="Demonstrate3" class="polaroid">
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                   <img src="https://static.igem.org/mediawiki/2018/b/b8/T--CCU_Taiwan--swot-2.png" style="width:100%">
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                   <div class="container">
 
                   <div class="container">
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                     <p>Figure3: SWOT Analysis</p>
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                     <p>Figure5: Three Bonds based on Spartan Analysis</p>
 
                   </div>
 
                   </div>
 
                 </div>
 
                 </div>
 +
 +
 +
<p class="first" id="ca3">Market analysis</p>  <br>
 +
<p class="description">&emsp;&emsp;We have already done a market survey of Liggreen. In response to the needs of the commodity market, we have discussed several practical business models and various analyses. Furthermore, we have visited several companies that produce laminated paper cups, as well as several companies that are concerned about new environmentally friendly materials. After these visits, our project has been further improved and more in line with social needs.</p>
 +
 
   <br>
 
   <br>
 
<p class="description">&emsp;&emsp;In the future, we will make Liggreen not only in the laboratory but also meet the production standards of industry. We will promote the products to the public and fulfill the concept of environmental protection.</p>
 
<p class="description">&emsp;&emsp;In the future, we will make Liggreen not only in the laboratory but also meet the production standards of industry. We will promote the products to the public and fulfill the concept of environmental protection.</p>

Revision as of 19:33, 17 October 2018

DEMONSTRATION

Yeasts & Enzymes

  In terms of gene design, our goal is mainly the production of plant enzymes. Therefore, we demonstrated the growth curve of the yeast and confirmed the enzyme production.

Figure1: ZeocinTM selection



His4 experiment

  In our experiments, we have successfully constructed pGAPZ A containing HIS4 vectors. As of yet, we have just successfully transformed pGAPZ A+HIS4+Px18 vector into P.pastoris. In the future, we will do some further researches on this experiment.

Figure2: Plasmid digest (EcoRI, Agel) after miniprep

Figure3: Plasmid digest(SalI) check

Figure4: pGAPZ A+HIS4+Px18 selection with SD plate

Properties analysis

  For our production methods, we refer to the current material production and get some engineering conditions from the company. Through these engineering conditions, we have designed some modeling and production line. We hope that Liggreen will be more in line with business needs. We hope that Liggreen will have a place in the market.
  In terms of engineering properties of Liggreen, we also completed two important parts.



PART 1.

Spontaneous reaction of monolignols & calculation of Flory-Stockmayer Theory of polymerization

  In our modeling, we constructed a theoretical calculation for our material. Based on published literature, we used Spartan to determine the spontaneous basis for our bond reaction, and then Flory-Stockmayer theory to provide a theoretical estimate of the polymerization. Through the establishment of the model, we can get theoretical data on Liggreen. In patent applications and product improvements, we can improve on this estimate through theoretical calculations and material measurements.

Figure5: Three Bonds based on Spartan Analysis



PART 2.

Production line

  We have designed a production line to produce Liggreen. Using existing fermentation tank systems and reaction tank assemblies on the market, we can construct our production line.



Figure5: Three Bonds based on Spartan Analysis

Market analysis


  We have already done a market survey of Liggreen. In response to the needs of the commodity market, we have discussed several practical business models and various analyses. Furthermore, we have visited several companies that produce laminated paper cups, as well as several companies that are concerned about new environmentally friendly materials. After these visits, our project has been further improved and more in line with social needs.


  In the future, we will make Liggreen not only in the laboratory but also meet the production standards of industry. We will promote the products to the public and fulfill the concept of environmental protection.