Difference between revisions of "Team:CCU Taiwan/Experiments"
| Line 156: | Line 156: | ||
<p class="first" id="ca1">Laboratory notebooks</p> | <p class="first" id="ca1">Laboratory notebooks</p> | ||
<p class="description">Our project aims to produce new material— LIGGREEN. In our experiment, we select <I>P. pastoris</I> as our strain to secrete enzymes which helps the polymerization of coniferyl alcohol. Besides, we divide into two groups to conduct experiment— gene sequence with or without Zeocin.<br><br> | <p class="description">Our project aims to produce new material— LIGGREEN. In our experiment, we select <I>P. pastoris</I> as our strain to secrete enzymes which helps the polymerization of coniferyl alcohol. Besides, we divide into two groups to conduct experiment— gene sequence with or without Zeocin.<br><br> | ||
| − | Here are the common steps of our experiment：<br>1. Binding our gene sequence by the transformation of E.coli <br>2. Extracting and purifying DNA purification from E.coli <br>3. Transforming the synthetic DNA into P. pastoris <br>4. Conducting western blot to detect our target enzyme <br>5. Purified target enzyme</p> | + | Here are the common steps of our experiment：<br>1. Binding our gene sequence by the transformation of E.coli <br>2. Extracting and purifying DNA purification from E.coli <br>3. Transforming the synthetic DNA into <I>P. pastoris</I> <br>4. Conducting western blot to detect our target enzyme <br>5. Purified target enzyme</p> |
<iframe src="https://static.igem.org/mediawiki/2018/5/5e/T--CCU_Taiwan--experimentv1.pdf" height="800" width="950" style="margin-left:20vw;"></iframe> | <iframe src="https://static.igem.org/mediawiki/2018/5/5e/T--CCU_Taiwan--experimentv1.pdf" height="800" width="950" style="margin-left:20vw;"></iframe> | ||
</div> | </div> | ||
Revision as of 00:09, 18 October 2018
EXPERIMENTS
Laboratory notebooks
Our project aims to produce new material— LIGGREEN. In our experiment, we select P. pastoris as our strain to secrete enzymes which helps the polymerization of coniferyl alcohol. Besides, we divide into two groups to conduct experiment— gene sequence with or without Zeocin.
Here are the common steps of our experiment：
1. Binding our gene sequence by the transformation of E.coli
2. Extracting and purifying DNA purification from E.coli
3. Transforming the synthetic DNA into P. pastoris
4. Conducting western blot to detect our target enzyme
5. Purified target enzyme