Difference between revisions of "Team:XJTLU-CHINA/Composite Part"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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        .front,
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<h1>Composite Parts</h1>
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<p>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<body style="background-color: rgb(84,146,197);">
  
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<div class="highlight decoration_background">
 
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<h3>Note</h3>
 
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
 
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<div class="container-fluid" style="background-color: rgb(84,146,197); padding: 40px 30px; margin-top: 35px">
  
  
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<div class="column full_size">
 
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<h3>Best Composite Part Special Prize</h3>
 
  
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<p>To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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    <div style="margin-top: 20px; width: 100px; float: left"></div>
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    <img src="https://static.igem.org/mediawiki/2018/1/1a/T--XJTLU-CHINA--lo.png" style="width: 140px; margin-top: 20px; float: left">
 +
    <h1 style="font-family:'Impact'; color: white; font-size: 80px; margin-top: 20px; margin-bottom: 50px; float: left; margin-left: 20px">
 +
        Composite Parts
 +
    </h1>
  
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<br><br>
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    <div class="container-fluid" style="background-color: white; box-shadow: 0px 0px 4px rgba(0,0,0,0.56); border-radius: 5px; margin-top: 200px; padding-bottom: 50px">
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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</div>
 
  
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        <div class="row" style="margin-top: 50px">
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            <div class="col-xs-1"></div>
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            <div class="col-xs-10">
 +
                <table class="table" style="border-right: 1px solid rgba(0,0,0,0.1); border-left: 1px solid rgba(0,0,0,0.1); border-bottom: 1px solid rgba(0,0,0,0.1)">
 +
                    <tr>
 +
                        <th>Part Number</th>
 +
                        <th>Part Name</th>
 +
                        <th>Function</th>
 +
                        <th>Length</th>
 +
                        <th>Designer</th>
 +
                    </tr>
 +
                    <tr>
 +
                        <th style="font-weight: 400"><a href="http://parts.igem.org/Part:BBa_K2619100">BBa_K2619100</a></th>
 +
                        <th style="font-weight: 400">Exosome marker</th>
 +
                        <th style="font-weight: 400">CD63 is fused with nluc to the measure the quantity of exosome by luminescence signal shown by nluc.</th>
 +
                        <th style="font-weight: 400">1241bp</th>
 +
                        <th style="font-weight: 400">Haoping Cheng</th>
 +
                    </tr>
 +
                    <tr>
 +
                        <th style="font-weight: 400"><a href="http://parts.igem.org/Part:BBa_K2619101">BBa_K2619101</a></th>
 +
                        <th style="font-weight: 400">Packaging device (L7Ae)</th>
 +
                        <th style="font-weight: 400">L7Ae is linked to the C-terminus of CD63 to package mRNA with C/D box during the formation of exosomes.</th>
 +
                        <th style="font-weight: 400">1113bp</th>
 +
                        <th style="font-weight: 400">Haohui Fang</th>
 +
                    </tr>
 +
                    <tr>
 +
                        <th style="font-weight: 400"><a href="http://parts.igem.org/Part:BBa_K2619102">BBa_K2619102</a></th>
 +
                        <th style="font-weight: 400">Packaging device (MS2)</th>
 +
                        <th style="font-weight: 400">MS2 can be fused with exosome-associated proteins CD63 to package mRNA with 3 loops during the formation of exosome. </th>
 +
                        <th style="font-weight: 400"></th>
 +
                        <th style="font-weight: 400"></th>
 +
                    </tr>
 +
                    <tr>
 +
                        <th style="font-weight: 400"><a href="http://parts.igem.org/Part:BBa_K2619003">BBa_K2619103</a></th>
 +
                        <th style="font-weight: 400">Booster</th>
 +
                        <th style="font-weight: 400">The tricistronic STEAP3-SDC4-NadB construct at a 1:1:1 ratio by which exosome secretion is supposed to enhanced by 15-fold to 40-fold in yield </th>
 +
                        <th style="font-weight: 400">2682bp</th>
 +
                        <th style="font-weight: 400">Shiyu Liu</th>
 +
                    </tr>
 +
                    <tr>
 +
                        <th style="font-weight: 400"><a href="http://parts.igem.org/Part:BBa_K2619104">BBa_K2619104</a></th>
 +
                        <th style="font-weight: 400">mRNA modification (nluc-C/D box-VEEV) </th>
 +
                        <th style="font-weight: 400">C/D box-VEEV can be added to 3'-UTR region of actively loading RNA to bind to RNA binding domain of packaging device with L7Ae and stablize mRNA. Nluc was added as reporter gene.</th>
 +
                        <th style="font-weight: 400">1016bp</th>
 +
                        <th style="font-weight: 400">Shiyu Liu</th>
 +
                    </tr>
 +
                    <tr>
 +
                        <th style="font-weight: 400"><a href="http://parts.igem.org/Part:BBa_K2619105">BBa_K2619105</a></th>
 +
                        <th style="font-weight: 400">mRNA modification  (nluc-3 loop-VEEV)</th>
 +
                        <th style="font-weight: 400">C/D box contains the structure of k-loop RNA motif which can be recognized by L7Ae. By engineering it at the 3'-UTR of cargo mRNA, CD63-L7Ae can load that RNA into exosome.</th>
 +
                        <th style="font-weight: 400">Peng Chai</th>
 +
                        <th style="font-weight: 400">72bp</th>
 +
                    </tr>
 +
                    <tr>
 +
                        <th style="font-weight: 400"><a href="http://parts.igem.org/Part:BBa_K2619006">BBa_K2619006</a></th>
 +
                        <th style="font-weight: 400">3 loop</th>
 +
                        <th style="font-weight: 400">3 loop-VEEV can be added to 3'-UTR region to  actively load RNA  by binding domain of packaging device with MS2.Nluc was added as reporter gene and VEEV is utilized for stablization.</th>
 +
                        <th style="font-weight: 400">1033bp</th>
 +
                        <th style="font-weight: 400">Shiyu Liu</th>
 +
                    </tr>
 +
                    <tr>
 +
                        <th style="font-weight: 400"><a href="http://parts.igem.org/Part:BBa_K2619007">BBa_K2619108</a></th>
 +
                        <th style="font-weight: 400">Modified RVG-Lamp2b</th>
 +
                        <th style="font-weight: 400">GNSTM was added to 5' of RVG to increase the drug-delivery efficiency of this targeting device.</th>
 +
                        <th style="font-weight: 400">1429bp</th>
 +
                        <th style="font-weight: 400">Haohui Fang</th>
 +
                    </tr>
 +
                    <tr>
 +
                        <th style="font-weight: 400"><a href="http://parts.igem.org/Part:BBa_K2619008">BBa_K2619109</a></th>
 +
                        <th style="font-weight: 400">nluc-C/D box</th>
 +
                        <th style="font-weight: 400">This part is used for measure the packaging ability of CD63-L7Ae--exosomal membrane fusion protein by fusing C/D box with reporter gene.</th>
 +
                        <th style="font-weight: 400">590bp</th>
 +
                        <th style="font-weight: 400">Miaoyan Li</th>
 +
                    </tr>
 +
                    <tr>
 +
                        <th style="font-weight: 400"><a href="http://parts.igem.org/Part:BBa_K2619009">BBa_K2619014</a></th>
 +
                        <th style="font-weight: 400">nSMase with Kozak</th>
 +
                        <th style="font-weight: 400">Exosome production boosting device.</th>
 +
                        <th style="font-weight: 400">1987bp</th>
 +
                        <th style="font-weight: 400">Haohui Fang</th>
 +
                    </tr>
 +
                </table>
 +
            </div>
 +
            <div class="col-xs-1"></div>
 +
        </div>
 +
 +
 +
 +
 +
 +
 +
    </div>
 +
 +
    <div style="background-color: white; box-shadow: 0px 0px 4px rgba(0,0,0,0.56); border-radius: 5px; margin-top: 50px; background-color: white; padding-bottom: 40px">
 +
        <div>
 +
 +
        </div>
 +
        <div style="text-align: center; padding-top: 40px">
 +
            <h1 style="margin: 0px auto; font-family:'Impact'; color: #337ab7">Collaborators and Supporters</h1>
 +
        </div>
 +
        <div class="container-fluid" style="margin-top: 50px">
 +
            <div class="row" style="padding: 0px 100px">
 +
                <div class="col-md-4" style="padding-left:5%; padding-right:5%;">
 +
                <div style="height: 100%">
 +
                    <img style="margin-top: 50px; width: 100%" src="https://static.igem.org/mediawiki/2018/3/3a/T--XJTLU-CHINA--qide.png">
 +
                    <img style="margin-top: 70px; width: 100%" src="https://static.igem.org/mediawiki/2018/3/3d/T--XJTLU-CHINA--NEB_logo.png">
 +
                </div>
 +
            </div>
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            <div class="col-md-4" style="padding:0 5%;">
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                <div style="height: 100%">
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                    <img src="https://static.igem.org/mediawiki/2018/b/b9/T--XJTLU-CHINA--genscript.png" style="width: 100%">
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Revision as of 00:25, 18 October 2018

Composite Parts

Part Number Part Name Function Length Designer
BBa_K2619100 Exosome marker CD63 is fused with nluc to the measure the quantity of exosome by luminescence signal shown by nluc. 1241bp Haoping Cheng
BBa_K2619101 Packaging device (L7Ae) L7Ae is linked to the C-terminus of CD63 to package mRNA with C/D box during the formation of exosomes. 1113bp Haohui Fang
BBa_K2619102 Packaging device (MS2) MS2 can be fused with exosome-associated proteins CD63 to package mRNA with 3 loops during the formation of exosome.
BBa_K2619103 Booster The tricistronic STEAP3-SDC4-NadB construct at a 1:1:1 ratio by which exosome secretion is supposed to enhanced by 15-fold to 40-fold in yield 2682bp Shiyu Liu
BBa_K2619104 mRNA modification (nluc-C/D box-VEEV) C/D box-VEEV can be added to 3'-UTR region of actively loading RNA to bind to RNA binding domain of packaging device with L7Ae and stablize mRNA. Nluc was added as reporter gene. 1016bp Shiyu Liu
BBa_K2619105 mRNA modification (nluc-3 loop-VEEV) C/D box contains the structure of k-loop RNA motif which can be recognized by L7Ae. By engineering it at the 3'-UTR of cargo mRNA, CD63-L7Ae can load that RNA into exosome. Peng Chai 72bp
BBa_K2619006 3 loop 3 loop-VEEV can be added to 3'-UTR region to actively load RNA by binding domain of packaging device with MS2.Nluc was added as reporter gene and VEEV is utilized for stablization. 1033bp Shiyu Liu
BBa_K2619108 Modified RVG-Lamp2b GNSTM was added to 5' of RVG to increase the drug-delivery efficiency of this targeting device. 1429bp Haohui Fang
BBa_K2619109 nluc-C/D box This part is used for measure the packaging ability of CD63-L7Ae--exosomal membrane fusion protein by fusing C/D box with reporter gene. 590bp Miaoyan Li
BBa_K2619014 nSMase with Kozak Exosome production boosting device. 1987bp Haohui Fang

Collaborators and Supporters

Location

Rm 363, Science Building

Xi'an Jiaotong-Liverpool University

111 Ren'ai Road, Suzhou, China

215123

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Get in touch

igem@xjtlu.edu.cn