Difference between revisions of "Team:TU-Eindhoven/Composite Part"

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                    <p>To realize our designed parts, we gratefully made use of the IDT Synthesis offer,
 
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                        allowing easy and fast access to the desired DNA constructs. All parts are codon
 
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                        optimized for expression in E. coli, the workhorse of our project. For all our composite parts,
 
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                        the comprising basic parts were registered separately on the Registry, to allow future iGEM teams to
 
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                        use our constructs in new assemblies. All parts we shipped to the registry are sequence confirmed.
 
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                        We nominated <a href="http://parts.igem.org/Part:BBa_K2812004">BBa_K2812004</a>, lysostaphin-thrombin linker-HlyA-His tag, as Best New Basic Part as we think
 
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                        that the realisation of secretable lysostaphin opens up a whole new area of projects and applications,
 
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                        since the issues and limitations associated with cell lysis are avoided. The T7-Lysostaphin-thrombin
 
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                        linker-HlyA-His part, <a href="http://parts.igem.org/Part:BBa_K2812005">BBa_K2812005</a>, has been nominated for Best New Composite Part, as it allows direct
 
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                        secretion of lysostaphin from the iGEM shipping backbone. This enables future teams to have a head start in
 
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                        their project.</p>
 
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                     <table class="parts-table">
 
                     <table class="parts-table">

Revision as of 03:05, 18 October 2018

Composite Parts

Composite Parts
Fave Part Name Type Description Designers Length
❤ BBa_K2812005 Composite Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 1496
BBa_K2812003 Composite Coding sequence for trunctated Lysostaphin regulated by T7-promoter Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls 776
BBa_K2812007 Composite Coding sequence for Pyosin S5 with HlyA and His6-tag regulated by AraC promoter Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 2288
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