Difference between revisions of "Team:TU-Eindhoven/Parts"

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                         optimized for expression in E. coli, the workhorse of our project. For all our composite parts,  
 
                         optimized for expression in E. coli, the workhorse of our project. For all our composite parts,  
 
                         the comprising basic parts were registered separately on the Registry, to allow future iGEM teams to  
 
                         the comprising basic parts were registered separately on the Registry, to allow future iGEM teams to  
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                         use our constructs in new assemblies. All parts we shipped to the registry are sequence confirmed.
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                         use our constructs in new assemblies. All parts we shipped to the registry are sequence confirmed.</p>
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                        We nominated <a href="http://parts.igem.org/Part:BBa_K2812004">BBa_K2812004</a>, lysostaphin-thrombin linker-HlyA-His tag, as Best New Basic Part as we think
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                        that the realisation of secretable lysostaphin opens up a whole new area of projects and applications,
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                        since the issues and limitations associated with cell lysis are avoided. The T7-Lysostaphin-thrombin
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                        linker-HlyA-His part, <a href="http://parts.igem.org/Part:BBa_K2812005">BBa_K2812005</a>, has been nominated for Best New Composite Part, as it allows direct
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                        secretion of lysostaphin from the iGEM shipping backbone. This enables future teams to have a head start in
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                        their project.</p>
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<p> During the 2018 Giant Jamboree, <a href="http://parts.igem.org/Part:BBa_K2812004">BBa_K2812004</a> was nominated for Best New Basic Part by the jury in the Overgrad competition. <a href="http://parts.igem.org/Part:BBa_K2812005">BBa_K2812005</a> was nominated for Best New Composite Part in the Overgrad competition.</p>
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<p> During the 2018 Giant Jamboree, <a href="http://parts.igem.org/Part:BBa_K2812004">BBa_K2812004</a> was nominated by the jury for Best New Basic Part in the Overgrad competition. <a href="http://parts.igem.org/Part:BBa_K2812005">BBa_K2812005</a> was nominated by the jury for Best New Composite Part in the Overgrad competition.</p>
  
 
                     <table class="parts-table">
 
                     <table class="parts-table">

Latest revision as of 19:39, 6 December 2018

Parts overview

To realize our designed parts, we gratefully made use of the IDT Synthesis offer, allowing easy and fast access to the desired DNA constructs. All parts are codon optimized for expression in E. coli, the workhorse of our project. For all our composite parts, the comprising basic parts were registered separately on the Registry, to allow future iGEM teams to use our constructs in new assemblies. All parts we shipped to the registry are sequence confirmed.

During the 2018 Giant Jamboree, BBa_K2812004 was nominated by the jury for Best New Basic Part in the Overgrad competition. BBa_K2812005 was nominated by the jury for Best New Composite Part in the Overgrad competition.

All Parts
Fave Part Name Type Description Designers Length
❤ BBa_K2812000 Basic Carbohydrate-binding domain from MpIBP Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 576
BBa_K2812001 Basic Coding sequence for trunctated Lysostaphin Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 738
BBa_K2812002 Basic pBAD-ara promoter - Arabinose inducible regulatory promoter Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls 71
BBa_K2812003 Composite Coding sequence for trunctated Lysostaphin regulated by T7-promoter Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls 776
❤ BBa_K2812004 Basic Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 1458
❤ BBa_K2812005 Composite Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 1496
BBa_K2812006 Basic Coding sequence for Pyocin S5 with HlyA and His6-tag Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 2217
BBa_K2812007 Composite Coding sequence for Pyosin S5 with HlyA and His6-tag regulated by AraC promoter Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 2288