Team:NYMU-Taipei/Model
FRET Model
Objective
This model aims to find out how much FRET protein should be added into our screening system.
Method
Chemical equilibrium is used to determine the florescence level and the minimal FRET protein activity required to produce the florescence that can be detected. We assume the portion of active protein in all protein is constant.
Result
The optimal ratio of the amount of one FRET protein to that of the other is the following:
The figures in the table indicates the optimal ratio of the protein on the top over the protein on the left.
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mCherry Expression Model
Objective
We made a gene that connects DKK1 promoter to mCherry. The gene expression rate of DKK1 promoter is affected by testosterone activity and affects the expression level of mCherry. The expression level of mCherry should be greater than a threshold so that its florescence can be detected by devices. In order to achieve this threshold, sufficient amount of testosterone should be in our screening system; this is the sensitivity of our screening system. This model aims to find out the sensitivity of our screening system.
Method
We simulate the kinetics of transcription signal and then simulate the expression of DKK1. Testosterone efficiency is used in this model instead of testosterone activity. Florescence decay is not considered in this model because ……..
Result
[DKK1] = 5 + 0.006 [DHT]^2 where [DKK1] indicate activity in ng/ml and [DHT] indicates activity in nM. [mCherry] = c_1 + c_2 [Testosterone]^2 where square brackets indicate activity in M, and c_1 and c_2 are constants to be determined. Note: This model is accurate only when [DHT] is less than 50 nM.
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FRET Model
Objective
This model aims to find out how much FRET protein should be added into our screening system.
Method
Chemical equilibrium is used to determine the florescence level and the minimal FRET protein activity required to produce the florescence that can be detected. We assume the portion of active protein in all protein is constant.
Result
The optimal ratio of the amount of one FRET protein to that of the other is the following:
The figures in the table indicates the optimal ratio of the protein on the top over the protein on the left.
Click Here For More Info