Team:NYMU-Taipei/experimentsandresults

HAIR TO STAY

Protocols

E.coli Protocols

Two-day Efficient Cloning Cycle

We used an efficient two day cloning cycle split into a "Light" day and a "Heavy" day.

Light Day

The light day consists of Colony PCR and liquid culture of colonies transformed from a previous day.

The 3-in-1
First, count the number of colonies you want to check. Then, do the following 3 things sequentially:
- Liquid culture
- 2nd time plate
- Colony PCR
(Use the same tip to add the template to these three things)
Make The Gel For Electrophoresis
Run Gel Electrophoresis To Check The Colony PCR Product

Plasmid Extraction

ballalalla

Plasmid Extraction

ballalalla

Plasmid Extraction

ballalalla