Team:NTHU Formosa/InterLab

IGEM 2018 Interlab Study

Overall To-Do List

Calibration 1：OD₆₀₀ Reference point - LUDOX Protocol

1.Record settings for standard measurements

2.Record measurements

3.Fill out corresponding Excel sheet

Calibration 2：Particle Standard Curve - Microsphere Protocol

1.Prepare Microsphere Stock Solution

2.Serial dilution

3.Measure Abs600 of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet

Calibration 3：Fluorescence standard curve - Fluorescein Protocol

1.Resuspend and dilute fluorescein

2.Serial dilutions

3.Measure fluorescence of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet

Cell Measurement protocol

Day 1:

Transform E.Coli DH5α with plasmids (all in pSB1C3)

Day 2:

pick 2 colonies per plate (5 mL LB medium + Chloramphenicol. Grow 16-18 hours @ 37 C and 220 rpm

Day 3:

Dilution cultures in LB+Chloramphenicol
Measure OD₆₀₀
Further dilute
Incubate
Abs600 and fluorescence measurement
Record measurements
Fill out corresponding Excel sheet

Protocol：Colony Forming Units per 0.1 OD₆₀₀ E.coli cultures