Team:NYMU-Taipei/Notebook
HAIR TO STAY
JUNE
week1
- Plasmid pET32a was extracted from competent cell BL21 DE3.
- We prepared competent cell solution buffer and made competent cell DH5-alpha.
JULY
week2
FRET MODEL
CELL MODEL
- Plasmid pUC19 was transformed into competent cell DH5-alpha.
- Plasmid pUC19 was extracted from competent cell DH5-alpha and checked by gel electrophoresis.
- Plasmid pUC19 was checked with restriction digestion(DpnI, EcoRI and BamHI).
week3
FRET MODEL
CELL MODEL
- Dkk1 promoter was amplified from HEK293 genomic DNA by KOD and DreamTaq polymerase PCR.
- DKK1 promoter PCR amplification checked by running an agarose gel(PCR failed).
- DH5-alpha pUC19 was stored in glycerol stock.
week4
FRET MODEL
week5
FRET MODEL
CELL MODEL
- KOD and DreamTaq polymerase PCR amplification of Dkk1 promoter from HEK293 genomic DNA.
- DKK1 promoter PCR amplification checked by running an agarose gel(Dream Taq success,KOD failed).
- troubleshooting of DKK1 promoter PCR amplification.
- KOD and DreamTaq polymerase PCR amplification of Dkk1 promoter from HEK293 genomic DNA.
- DKK1 promoter PCR amplification checked by running an agarose gel(Dream Taq success, KOD success).
AUGUST
week6
FRET MODEL
CELL MODEL
week7
FRET MODEL
CELL MODEL
week8
FRET MODEL
CELL MODEL
week9
FRET MODEL
CELL MODEL
SEPTEMBER
week10
FRET MODEL
CELL MODEL
week11
FRET MODEL
CELL MODEL
week12
FRET MODEL
CELL MODEL
week13
FRET MODEL
CELL MODEL
OCTOBER
week14
FRET MODEL
CELL MODEL
week15
FRET MODEL
CELL MODEL
week16
FRET MODEL
CELL MODEL