Exosome biogenesis

This year, we decided to engineer human embryonic kidney cells (HEK293T) to produce engineered exosomes. we genetically modified HEK293T to increase the production of exosome.

Exosome construct

We also enabled the transfection and protein expression of extracellular mRNA in living organism’s neuron by using two mRNA modifications.

Exosome delivery

Exosome is a kind of cell-derived vesicles with diameters between 30 nm ~100 nm and is naturally capable of passing the BBB. Our project involves three exosome device modification: RNA packaging, target cell characterization and exosome delivery.