Promoter (J23100, Anderson Strong)

Constitutive promoter family

Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. J23119 is the "consensus" promoter sequence and the strongest member of the family. These promoter parts can be used to tune the expression level of constitutively expressed parts.

RBS (B0034, Ribosome Binding Site)

RBS based on Elowitz repressilator.

Designed by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.

Terminator (B0010)

Transcriptional terminator consisting of a 64 bp stem-loop.

Designed by: Randy Rettberg

Terminator (B0012)

Transcription terminator for the E. coli RNA polymerase.

Designed by: Reshma Shetty

GBP (K2885001, Gold-Binding Polypeptide)

http://parts.igem.org/Part:BBa_K2885001

Gold-binding polypeptide (GBP), a genetically designed peptide, possesses unique and specific interactions with a gold surface, resulting in improved enzyme stability and activity. Antibodies can be immobilized on a electrochemical gold chip using GBP and protein that endows the orientation of antibody. The GBP composed of three repeats of the sequence MHGKTQATSGTIOS serves as the anchoring component on the basis of its strong binding properties with gold.

ProG (K2885000, Protein G)

http://parts.igem.org/Part:BBa_K2885000

Sequence of genes that encode Protein G (ProG). Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fab and Fc region. The DNA fragments encoding ProG were amplified by the genomic DNA of Staphylococcus aureus Mu50 as a template.

GBP-ProG (K2885002, Gold Binding Protein, Protein G Fusion Protein)

http://parts.igem.org/Part:BBa_K2885002

The GBP-ProG fusion protein was derived from genetically modified recombinant vector coding GBP and ProG. The resulting GBP-ProG was directly self-immobilized onto gold surfaces via the GBP portion, followed by the oriented binding of antibodies onto the ProG domain targeting the Fc region of antibodies.

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