Project Description

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Exosome biogenesis

This year, we decided to engineer human embryonic kidney cells (HEK293T) to produce engineered exosomes. we genetically modified HEK293T to increase the production of exosome.

RNA Packaging

Since exosomes have randomicity in substances packaging, we modified exosomes to be capable of packaging our designed therapeutic mRNA in larger amount. To achieve this goal, a RNA-protein interaction strategy was utilized.

Exosome delivery and targeting

Exosome is a kind of cell-derived vesicles with diameters between 30 nm ~100 nm and is naturally capable of passing the BBB. Our project involves three exosome device modification: RNA packaging, target cell characterization and exosome delivery.

RNA stabilization

Since exosomes have randomicity in substances packaging, we modified exosomes to be capable of packaging our designed therapeutic mRNA in larger amount. To achieve this goal, a RNA-protein interaction strategy was utilized.