Promoter (J23100, Anderson Strong)

Constitutive promoter family

Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. J23119 is the "consensus" promoter sequence and the strongest member of the family. These promoter parts can be used to tune the expression level of constitutively expressed parts.

RBS (B0034, Ribosome Binding Site)

RBS based on Elowitz repressilator.

Designed by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.

Terminator (B0010)

Transcriptional terminator consisting of a 64 bp stem-loop.

Designed by: Randy Rettberg

Terminator (B0012)

Transcription terminator for the E. coli RNA polymerase.

Designed by: Reshma Shetty

GBP (K2885001, Gold-Binding Polypeptide)

http://parts.igem.org/Part:BBa_K2885001

This part encodes gold binding polypeptide (GBP). GBP is consisted of 14 amino acids (MHGKTQATSGTIOS) triplication.

ProG (K2885000, Protein G)

http://parts.igem.org/Part:BBa_K2885000

This part encodes Protein G (ProG). The DNA sequences encoding ProG used in our experiment were derived from the genomic DNA of Staphylococcus aureus G418